Search PubMed⌕ Search

Biomedical subjects

T Nishida

Publications and source records attributed to T Nishida.

At least 649 records · Page 36Linked to original sources
PubMed1984

[Evaluation of cancer chemotherapy in primary ovarian cancer in rats--observation of histological effect by light and electron microscopy].

In order to evaluate the effects of ifosfamide as an anticancer agent, ifosfamide was administered to rats with ovarian cancer induced by DMBA (7,12-dimethylbenz (a) anthracene). Four to five weeks after the chemotherapeutic treatment 16 rats were sacrificed and then tumor sizes were compared before and after the treatment, and in addition morphologic changes were investigated by light and transmission electron microscopy. Of 16 rats, the reductions in tumor size were recognized in six cases. A histologic effect was observed in both the tumor decreased group and the increased group. One of the latter group showed excellent histological chemotherapeutic effect. On light microscopic observation, the histologic effects seen were reduction in the amount of neoplastic tissues and proliferation of connective tissues. On transmission electron microscopic observation, destruction of tumor cells and a reduction in the amount of ribosome and chromatin were recognized as the effects of the anticancer drug. Multinuclear giant cells, presumably derived from the mesenchym, were found among the connective tissues in one case showing a remarkable histologic effect.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1984

[Histological evaluation of cancer chemotherapy on rat ovarian cancer].

To evaluate the effect of cis-diamminedichloroplatinum (DDP), the drug-induced changes of tumor tissue were observed morphologically on a primary ovarian cancer in rat. In order to avoid artificial damages of the tissue, trans-aortic perfused fixation was demanded for light microscopy. The results obtained were as follows; 1) various degrees of cancer cell damages, presumably caused from different sensitivities of the each cell and vascularization, were observed 2) vascular networks were affected to decrease by the cytotoxic agent 3) the tumor size was occasionally decided by a proliferation of connective tissues in which survived cancer cells were found 4) an increase of histiocytes played a notable role to sweep out the damaged cells. A histologic examination at second look surgery in a clinical case reflected these results.

Animals↗
PubMed1984

[Cytodiagnosis of ovarian cancer--usefulness of peritoneal washings in histological diagnosis].

To determine the clinical significance of intraperitoneal cancer cells, comparative cytologic studies on ovarian cancer cells in peritoneal washings and ascites were performed. The materials obtained from 6 patients with ovarian cancer were examined by light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and the results achieved were as follows: Ovarian cancer cells were recognized in both peritoneal washings and peritoneal effusion. It was easier to get a diagnosis from the cytologic features of the peritoneal washings than those of the effusion, because of the fewer contaminants such as fibrin, red blood cells and other degenerated tissues. Despite the presence of clustered cells in the washings, the nuclei and cytoplasmas were clearly seen. On SEM observation of the clustered cells from the effusion, red blood cells and fibrin were seen to adhere to the surface of cancer cells. Ultrastructural observation of cancer cells by TEM confirmed the fact that the activity of the cells was maintained even in ascites.

Adult↗
PubMed1984

[Spindle cell carcinoma of the penis--a case report].

Over a 2-month period, a 34-year old man with phimosis developed a pedunculated tumor, measuring 4 X 2.5 X 2 cm in his glans penis. Microscopically the bulk of the tumor was composed of spindle-shaped cells, however, at some parts of the tumor, squamous cell cysts or nests, consistent with well-differentiated squamous cell carcinoma, were noted. Atypical epithelial cells merged imperceptibly with the underlying spindle-shaped cells. The histological diagnosis of spindle cell carcinoma, an uncommon variant of squamous cell carcinoma, was made. Spindle cell carcinoma arising from the penis is very rare and only two reports are available at present.

Adult↗
PubMed1984

[Approach to the diagnostic colposcopy--a scoring method of colposcopic pattern].

In order to arrive at a final diagnosis of early cervical cancer by colposcopy alone, a colposcopic scoring system was applied to 172 patients with the disease. Each colposcopic pattern was scored from one point (for white epithelium) to 9 (for invasive cancer). The results obtained were as follows; 1) With these scoring criteria, stage 0 cancers were restricted to below 10 points and were characterized colposcopically by white epithelia with gland openings 2) In stage I-a lesion, atypical vessels, punctuation by irregular arrangement of the dots and concentration of abnormal gland openings were usually observed, and these findings were combined with each other to show a more complicated colposcopic pattern reflected in 11 to 18 points of the score 3) Frank invasions were indicated by 19 points or more, and punctuations or mosaics were seldom found in this stage, although the early "I-b" cancer was difficult to distinguish from I-a with this method 4) The diagnoses from this scoring system were identical with the final diagnoses confirmed by surgical methods in 66.7% of stage 0, 59.4% of I-a and 76.5% of I-b.

Cervix Uteri↗
PubMed1983

Fibronectin. A new therapy for corneal trophic ulcer.

Fibronectin, a glycoprotein, is present in plasma and extracellular matrix and is responsible for cellular adhesion. With the use of affinity chromatography, we purified plasma fibronectin from two patients with trophic corneal ulcer and persistent epithelial defect after conventional therapy was ineffective. Reepithelialization began three days after initiation of treatment with autologous purified fibronectin eyedrops, and the epithelial defects disappeared completely within three weeks. No recurrence was observed after treatment ended. By modifying the original chromatographic procedure, we were able to obtain purified fibronectin in two hours, which allowed us to treat outpatients as well as inpatients.

Adult↗
PubMed1983

A new avian erythroblastosis virus, AEV-H, carries erbB gene responsible for the induction of both erythroblastosis and sarcomas.

The genome structure of a newly isolated avian erythroblastosis virus, AEV-H, was analyzed. Using DNA probes specific for the LTR sequence of SR-RSV-A, and for the erbA gene and the erbB gene of the ES4 strain of AEV, we have shown that the genome of AEV-H is 35S in size and carries the erbB gene but not the erbA gene. Comparison of the restriction sites of molecularly cloned AEV-H DNA with that of cloned DNA of the associated virus revealed that the env gene of the associated virus was replaced with the erbB gene to generate AEV-H. The genome structure of AEV-H is, therefore, determined to be 5'-gag-pol-erbB-3'. Moreover, we have isolated a mutant of AEV-H, td-130, that can induce sarcomas but not erythroblastosis in chickens. The restriction analysis of proviral DNA of the td-130 showed that it carries a deletion of about 150 to 200 nucleotides in the erbB gene. These data indicate that the erbB protein is responsible for both erythroblastosis and sarcomas.

Alpharetrovirus↗
PubMed1983

The erbB gene of avian erythroblastosis virus is a member of the src gene family.

The erbB gene of an avian erythroblastosis virus, AEV-H, was determined to be 1812 nucleotides long and was predicted to code for a protein of 67,638 daltons. Unexpectedly, a sequence of 285 amino acids in the middle of the protein showed a significant homology (38%) with the sequence in the carboxy terminus of p60src. The nucleotide sequence of a mutant of AEV-H, td-130, which induces sarcomas but not erythroblastosis in chicken, was also analyzed. A deletion of 169 nucleotides was identified in the 3' half of the erbB gene, indicating that the gene codes for a truncated protein with the predicted molecular weight of 46,667. These findings suggest that the homologous domain of erbB protein with its N-terminal portion is sufficient for the transformation of fibroblasts and that one-third of the carboxy-terminal domain has a key role for the transformation of erythroid cells.

Alpharetrovirus↗
PubMed1983

Hanganutziu-Deicher heterophile antigen in human retinoblastoma cells.

We investigated the possible existence of Hanganutziu-Deicher antigen (HD antigen) on retinoblastoma cells by means of indirect membrane immunofluorescence and a complement-dependent antibody-mediated cytotoxicity test. We prepared antiserum in the chicken by immunization with purified equine hematoside highly specific to HD antigen. Three kinds of retinoblastoma cell lines, Y-79, WERI-Rb 1, and TOTL-1, showed positive membrane staining. Y-79 and TOTL-1 cells were also confirmed by the cytotoxicity test to possess HD antigen. Retinoblastoma cells freshly isolated from two patients had a positive membrane immunofluorescence. These results indicated that HD antigen was expressed in human retinoblastoma cells in vitro as well as in vivo.

Antigens, Heterophile↗
PubMed1983

Fibronectin promotes epithelial migration of cultured rabbit cornea in situ.

We investigated the effect of fibronectin on epithelial migration onto the stroma in cultured rabbit cornea. Rabbit plasma fibronectin was purified by affinity chromatography using gelatin-Sepharose 4B, and its purity was confirmed by SDS polyacrylamide slab gel electrophoresis. Antibody against rabbit plasma fibronectin raised in guinea pigs formed a single precipitin line against rabbit plasma and purified rabbit plasma fibronectin by Ouchterlony double diffusion test. When rabbit cornea was cut into small blocks and cultured in TCM-199 medium alone, corneal epithelial cells began to migrate on the cut edge of the corneal stroma. The addition of purified rabbit plasma fibronectin to the culture medium significantly enhanced epithelial migration. The degree of enhancement depended on the amount of fibronectin added. When guinea pig IgG anti-rabbit plasma fibronectin was added, epithelial migration was significantly inhibited when compared with that in control cultured corneal blocks. The results demonstrate that fibronectin promotes epithelial migration in the cornea and thus plays an important role in corneal wound healing.

Animals↗