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T Nishida

Publications and source records attributed to T Nishida.

At least 505 records · Page 28Linked to original sources
PubMed1989

Amino-terminal region of human macrophage colony-stimulating factor (M-CSF) is sufficient for its in vitro biological activity: molecular cloning and expression of carboxyl-terminal deletion mutants of human M-CSF.

Human T lymphoblastoid cell line CEM-ON belongs to a helper/inducer subclass and secretes M-CSF into medium constitutively. We have isolated a full-length cDNA clone for this factor from a cDNA library of this cell line. The cDNA was 2.5 kb and coded for a 554 amino acid polypeptide precursor including signal sequence. The Northern blot analysis showed that the major transcript of M-CSF is about 4.2 kb. We have studied the expression of not only the wild type plasmid, but also the five C-terminal deletion mutants encoding N-terminal 154, 163, 170, 177, and 185 amino acid residues in monkey COS-1 cells. The results showed that at least C-terminal 377 amino acid residues of human M-CSF are not essential to manifest the in vitro biological activity on murine bone marrow cells.

Amino Acid Sequence↗
PubMed1989

Purification, microheterogeneity, and stability of human lipid transfer protein.

A method for the purification of lipid transfer protein (LTP) from human plasma was developed with the aid of succinylated low density lipoprotein-Sepharose affinity column chromatography. The purified LTP exhibited a single main band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. However, upon isoelectric focusing on polyacrylamide gel, the preparations consistently showed nine bands with isoelectric points ranging from 4.6 to 5.4. The treatment of LTP with Clostridium perfringens neuraminidase shifted these multiple bands toward higher pH regions due to the release of sialic acid. Extensive treatment with neuraminidase resulted in the appearance of a major band with the isoelectric point of 5.6. The purified LTP was rapidly inactivated upon incubation at 37 degrees C due to the denaturation at the "air"-water interface. Various factors promoting or preventing this interfacial denaturation were elucidated. When purified LTP was stored at 4 degrees C, plasma neuraminidase co-purified with LTP became activated, resulting in the gradual desialylation of LTP. It seemed that the LTP preparations of apparent homogeneity are associated with a trace amount of an inactive form of plasma neuraminidase. The inclusion of 4 mM 2-mercaptoethanol or 0.2% EDTA in the storage media completely prevented the activation of plasma neuraminidase. These agents, however, did not significantly inhibit the already activated neuraminidase. When LTP was stored at -20 degrees C in very low ionic strength media, such as 0.001% EDTA (pH 7.4) and at high protein concentrations, the loss of the activity was minimal even after prolonged storage.

Carrier Proteins↗
PubMed1989

Dexamethasone regulation of the expression of cytokine mRNAs induced by interleukin-1 in the astrocytoma cell line U373MG.

BSF-2/IL-6, GM-CSF and IL-1 beta mRNAs were induced by recombinant IL-1 in human astrocytoma cell line U373MG. The induction of BSF-2/IL-6 and IL-1 beta mRNAs did not require de novo protein synthesis while that of GM-CSF mRNA required a newly synthesized protein. Dexamethasone inhibited the induction of these cytokine mRNAs by IL-1. This process seems to require continued protein synthesis. These results suggest that the production of these cytokines are positively and negatively controlled by IL-1 and glucocorticoids, respectively, in astrocytes.

Astrocytoma↗
PubMed1989

Changes in lectin binding pattern of gonads of developing mice.

Changes in lectin binding of developing fetal mouse testes and ovaries were examined by light and electron microscopy, with much attention paid particularly to those in carbohydrates of germ cells. Characteristic binding patterns were observed with three lectins (BPA, GS-I, and GS-II) in the germ cells and the somatic cells during the process of testicular and ovarian development. GS-I and BPA, which showed similar binding patterns, preferentially bound to the plasma membrane and small dense bodies (SDB) of germ cells in both testes and ovaries during the 12th to 14th day post coitum (p.c.). In the fetal testes on day 16 p.c., the reaction with both GS-I and BPA completely disappeared. While, in the ovaries, a weak reaction with these lectins was retained as it was in germ cells until the 16th day p.c. The reaction with GS-II was restricted to Sertoli cells in the fetal testes during the 12th to 14th day p.c., and thereafter disappeared on day 16 p.c. The distribution of GS-II binding sites was in agreement with that of the glycogen granules. No positive staining with GS-II was seen in the ovaries throughout their development. These results indicate that certain glycoconjugates containing D-galactose and N-acetyl-D-galactosamine residues are expressed on the cell surface and in the SDB of germ cells during the period of the 12th to 14th day p.c., and that striking changes in function as well as in structure may take place in both germ cells and somatic cells during the 14th to 16th day p.c. in association with testicular and ovarian development.

Animals↗
PubMed1989

Molecular cloning and expression of rat interleukin-1 alpha cDNA.

A cDNA sequence coding for rat interleukin-1 alpha (IL-1 alpha) has been isolated from a cDNA library that was prepared with mRNA derived from LPS-stimulated rat peritoneal macrophages by using human IL-1 alpha cDNA as a probe. The rat cDNA encodes a 270 amino acid residue protein which is homologous (65%) to human IL-1 alpha. The rat cDNA sequence under SV40 early promoter directed the synthesis of biologically active IL-1 in monkey COS-1 cells. Rat IL-1 alpha mRNA is not expressed in spleen, lung, liver or brain, and is also not expressed in these organs of LPS-treated rat except spleen. This suggests that IL-1 alpha is not produced constitutively in various tissues and LPS is not sufficient to induce IL-1 alpha in most tissues. Our data indicate that the IL-1 activities which have been reported to be produced in the brain are not of alpha type. We have constructed a plasmid expressing the carboxy terminal 156 amino acids in Escherichia coli. Recombinant rat IL-1 alpha produced in COS cells or E. coli has cytotoxic activity against the human melanoma cell line A375S1 (GIF activity), which has been reported to be sensitive to human IL-1 alpha and IL-1 beta. This suggests that GIF activity is common to IL-1s derived from various sources.

Amino Acid Sequence↗
PubMed1989

Involvement of Ca2+ release and activation of phospholipase A2 in mitochondrial dysfunction during anoxia.

During anoxic incubation, depletion of mitochondrial ATP was followed by release of Ca2+ with concomitant increase in the rate of state 4 respiration due to disruption of the diffusion barrier against protons. The external addition of ATP and its non-metabolizable analog, beta,gamma-methylene adenosine 5'-triphosphate, prevented both the release of Ca2+ and increase in the rate of state 4 respiration. Addition of EGTA, which did not prevent release of the ion, resulted in little increase in the respiration rate. Addition of an inhibitor of mitochondrial phospholipase A2, such as quinacrine, dibucaine, or chlorpromazine, also prevented increase in the respiration rate without affecting Ca2+ release from mitochondria during anoxic incubation. Non-esterified polyunsaturated fatty acids were also found to be liberated from anoxic mitochondria. External addition of the ATP-analog, EGTA, and inhibitors of phospholipase A2 suppressed the liberation of non-esterified polyunsaturated fatty acids. Melittin and Ca2+, which activate phospholipase A2, increased the rate of state 4 respiration and the liberation of fatty acids. These findings support the hypothesis proposed previously that the following sequence changes occurs in mitochondria during anoxia; depletion of ATP, liberation of free calcium from mitochondria, and disruption of the diffusion barrier against H+ of the inner membrane. The results also indicate another event; activation of phospholipase A2 by release Ca2+ which results in H+ leakiness of the inner membrane.

Animals↗
PubMed1989

Cloning and sequencing of an Escherichia coli gene, nlp, highly homologous to the ner genes of bacteriophages Mu and D108.

An nlp (Ner-like protein) gene was isolated from Escherichia coli. The nucleotide sequence of a 1,342-base-pair chromosomal DNA fragment containing the nlp gene was analyzed. It contained two open reading frames; one encoded 91 amino acid residues with an Mr of 10,361, and the other (ORFX) encoded 131 amino acid residues of the carboxyl-terminal region of a truncated polypeptide. The amino acid sequence deduced from the DNA sequence of nlp was highly homologous (62 to 63%) to the Ner proteins of bacteriophages Mu and D108. The amino-terminal region of Nlp deduced from the complete open reading frame contained a presumed DNA-binding region. The nlp gene was located at 69.3 min on the E. coli genetic map.

Amino Acid Sequence↗
PubMed1989

Formation of male and female sex cords in gonadal development of C57BL/6 mouse.

The sex cords of male and female fetal C57BL/6 mice were studied by light and transmission electron microscopy to elucidate the origins of Sertoli cells (male) and follicle cells (female) in detail. In the testes of fetal mice from day 12 to day 14 post coitum (p.c.), PAS-positive substances were detected exclusively throughout the cytoplasm of Sertoli cells. On day 12 p.c., pre-Sertoli cells, identified by PAS-reaction, formed irregular and cord-like arrangements around germ cells. The arrangements were not associated with the coelomic epithelium. Thus, it is suggested that Sertoli cells are originated from the mesonephric tissues. In the ovaries, germ cell cords were still not observed at a stage immediately following the gonadal sex differentiation in male. On about day 15 p.c., connective tissues including many capillaries penetrated into the ovaries, resulting in obvious formation of germ cell cord-like arrangements. At the same time, the coelomic epithelium-derived cells (CEd cells) as well as the mesonephros-derived cells (Md cells) invaded the adjacent arrangements. It is concluded that the follicle cells are originated most from Md cells and some from CEd cells.

Animals↗
PubMed1989

Fine structure of the mandibular gland in volcano rabbit.

The mandibular glands of 6 male and 6 female volcano rabbits were examined by means of light and transmission electron microscopy. The acinar cells of the glands were seromucous in nature, and contained faintly basophilic granules. The cells were classified into the light cells containing granules of low or moderate densities and the clear cells having polygonal granules of low density. The preacinar cells were occasionally observed at the site between acinus and intercalated duct. These cells had many weakly basophilic granules which contained fine granular materials of moderate density. The intercalated ducts were composed of light cells containing cored granules. The striated duct cells consisted of light cells and dark cells. Both of them contained a few vacuoles and vesicles, but no secretory granules. No sex-and age-related differences were observed in the mandibular gland of the volcano rabbit. The mandibular gland of the volcano rabbit was similar to the rabbit mandibular gland rather than the pika mandibular gland morphologically.

Animals↗
PubMed1989

Vascular morphology of the golden hamster spermatic cord.

The morphology of the convoluted testicular artery and the pampiniform plexus of the golden hamster was studied by light microscopy and corrosion cast techniques combined with scanning electron microscopy. The artery was found to be totally enclosed by the pampiniform plexus, except for minor superficial areas where the artery was exposed. Although no direct connection between the artery and the vein was found in the area of apposition, the arterial and venous walls reduced their thickness by sharing a single tunica adventitia, which seemed well suited to the transfer of substances by diffusion. Many band-like structures of the venous walls were found in the deep part of the spermatic cord, suggesting that these may act as barriers to slow down the venous blood velocity. The venous wall here and there showed a stick-like endothelial bridge, suggesting that it may prevent the veins from over distension. In addition to the close relation between the artery and the vein, lymphatic vessels and mast cells were distributed widely within the connective tissue of the arterio-venous walls and venous walls. Mast cells were situated mainly in the area of apposition, especially at the base of the protruding venous wall. These morphological findings suggest that mast cells may be involved in the counter-current transfer mechanism in the spermatic cord of the golden hamster.

Animals↗
PubMed1989

Ultrastructural identification of axons supplying the M. retractor phalli cranialis in chickens.

The chicken m. retractor phalli cranialis classified as a smooth muscle was examined by electron microscopy to study the innervation of this muscle using the dichromate/chromate fixation method for demonstration of biogenic amines (Tranzer and Richards, 1976). Approximately two axon complexes per 100 muscle cells were found in both sexes. The axon varicosities were divided into the following four types; varicosities with, 1) small clear vesicle and chromaffin-negative large granular vesicle (cholinergic), 2) chromaffin-positive, 6-hydroxydopamine (6-OHDA) susceptible small and large granular vesicle (adrenergic), 3) chromaffin-positive, 6-OHDA resistant pleomorphic and large granular vesicle (aminergic?), or 4) chromaffin-negative large opaque vesicle (peptidergic?). These results suggest that non-adrenergic, non-cholinergic nerve may also play an important role in the innervation of m. retractor phalli cranialis as well as of the smooth muscle in mammalian intestine.

Animals↗
PubMed1989

Therapeutic and staging operations for endometrial carcinomas.

A staging error can lead to a treatment failure in the management of patients with malignant diseases. The actual progression of endometrial carcinoma was postoperatively examined in twenty patients with clinical stage II disease classified by FIGO (International Federation of Gynecology and Obstetrics) criteria. In seven of 20 patients (35%), extrauterine lesions were revealed at surgery. These seven stage-up tumors included three grade 3 endometrioid cancers, three uterine papillary serous carcinomas (UPSC) and one grade 2 endometrioid tumor with deep myometrial invasion. The sites of the extrauterine lesions were determined. The grade 2 cancer was associated with parametrial invasions and positive pelvic lymph nodes. One grade 3 endometrioid tumor and two UPSCs had positive periaortic lymph nodes. Omental involvements were revealed in one grade 3 cancer and two UPSCs, indicating that the pattern of spreading of endometrial cancer with a malignant histology is similar to the spreading of ovarian cancer. Since the both endometrium and ovarian surface epithelium have a common histologic origin in the early embryonic stage, a similar biological characteristics of these tumors are suggested. From the results, it is recommended that a radical hysterectomy with periaortic lymph node dissection, omentectomy and peritoneal washing cytology be performed for endometrial cancer with a malignant histology or deep myometrial invasion to obtain the actual staging which is necessary for maximal curative potential.

Adult↗
PubMed1989

31P-NMR-spectroscopy measurements of energy metabolism of in vivo growing ascites tumours following addition of glucose.

The cellular ATP content and the phosphorylation potential, defined as the ATP, ADP and inorganic phosphate (Pi) ratios, of exponentially growing Ehrlich ascites tumour cells were compared with cells at the plateau phase of growth. These phosphorus compounds were measured using 31P-NMR-spectroscopy immediately after removal of the cell material from the host and in their ascites fluid reflecting in vivo growth conditions. Reaching the plateau phase of growth, the ATP content and the phosphorylation potential decreased. Upon addition of glucose, the phosphorylation potential immediately increased. We concluded that the reduced phosphorylation potential was due to a limited availability of glucose in spite of the nearly normal blood glucose concentration found. An increasing diffusion distance from the host to all parts of the tumor is a possible reason for that.

Adenosine Triphosphate↗
PubMed1989

Ifosfamide, adriamycin and cisplatin (IAP) plus bleomycin (B) combination chemotherapy in patients with recurrent cancer of the uterine cervix.

A combination chemotherapy of ifosfamide, adriamycin and cisplatin (IAP) plus bleomycin (IAP plus B) was used to treat nine patients with recurrent cervical cancer of the uterus. All patients had received conventional radiation therapy with or without hydroxyurea as a potential radiation sensitizer, and seven of them had measurable disease in a previous irradiated field (4 cases) or distant area (3 cases). The tumors included 5 squamous cell carcinomas (non keratinizing: 2, keratinizing: 3), two adenosquamous (including one glassy cell carcinoma), one adenocarcinoma (endometrioid type) and one argyrophil cell carcinoma. All histologic type cancers except argyrophil cell carcinoma responded to IAP plus B treatment. In seven evaluable cases, three complete responses (CR) and 3 partial responses (PR) were recorded (response rate was 85.7%), including 3 responded tumors within previously irradiated pelvic tissue. The median duration of CR was over 7 months and that of PR was 3.5 months. Side effects of IAP plus B were severe in hematologic toxicity (100% of grade 4 leucopenia) but acceptable, permitting five courses of treatments at four week intervals. The results obtained were encouraging, although therapeutic benefits of IAP plus B were not evident in a patient with argyrophil cell carcinoma and the intractable nature of this histologic type cancer in the uterine cervix was again emphasized.

Adenocarcinoma↗
PubMed1989

[Acute myelomonocytic leukemia (M4) with CD19 antigen expression, eosinophilia and basophilia in bone marrow].

A 12-year old boy was admitted to Saitama Children's Medical Center because of fever and epistaxis. He had leukocytosis (WBC 40,800/microliters, blast 75%), anemia, thrombocytopenia and high levels of serum LDH, lysozyme, Vitamin B12, and plasma histamine. Bone marrow aspiration revealed hypercellular marrow with 31.2% blasts, 15.2% eosinophils, and 14.2% basophils. Blasts had Auer rods and were positive for peroxidase and negative for alpha-naphthyl butyrate esterase and PAS stainings. Ia, CD13 (My7), and CD19 (B4) antigens were expressed on his leukemic cells. Chromosomal study showed 46, XY, t(7;8) (q35;q22), del(9) (q13q22). Southern blot analysis using immunoglobulin constant region (C) probes revealed germline patterns of C mu, C kappa, C lambda, and breakpoint cluster region. A diagnosis of acute myelomonocytic leukemia (AMMoL, M4) was made. He attained a complete remission with daunorubicin and cytarabine, and 6 months later he received bone marrow transplantation from HLA-identical sister. This case had the common breakpoint 8q22 with ANLL with t(8;21) (q22;q22), and was unique AMMoL with proliferation of eosinophils and basophils in bone marrow.

Antigens, CD19↗
PubMed1989

[Clinicopathological study of pseudomyxoma peritonei].

Twelve cases of pseudomyxoma peritonei in Kurume University Hospital and its affiliated hospitals were clinicopathologically analysed. The patients were 42 to 81 years old, and the averaged age was 65.1 years. One case was before menopause. As to past history, one had undergone simple hysterectomy for myoma uteri previously, another patient (No. 8) was diagnosed as having mucinous adenocarcinoma of the appendix and two others (cases No. 2 and 7) had had treatment for pleurisy and peritonitis. Histologically, 3 cases were diagnosed as benign, 4 were borderline malignancy, and 5 were frankly malignant. Six cases were cytologically examined. Four of 6 were recognized as malignant cases. It was thought the diagnosis should be done by both cytological and radiological examination. It was suggested by the past history and histological infiltration of inflammatory cells in cases No. 2 and 7 that the histogenesis of pseudomyxoma peritonei was related to the inflammation with mucinous tumors. The relationship between histological findings and prognosis was unclear. Three cases were treated by surgery only, 6 cases by surgery and chemotherapy, and the other 3 cases by surgery, chemotherapy and washing intraperitoneally with dextran. The five year survival rate for cases treated by surgery and chemotherapy was 40.0%, including 2 patients still living more than 10 years later. All three cases treated by washing intraperitoneally with dextran survived, but for less than 5 years. The result of treatment by washing with dextran was thought to be an effective therapy for pseudomyxoma peritonei.

Adenocarcinoma, Mucinous↗
PubMed1989

[Effects of anticancer agents on 7, 12-dimethylbenz (a) anthracene induced rat ovarian cancer cell line (DMBA-OC-1) and human ovarian serous adenocarcinoma cell line (KOC-1S)].

The antitumor activities of five anticancer drugs, at IC50 dosages cisplatin (CDDP), adriamycin (ADM), etoposide (VP-16), mitomycin C (MMC) and carboplatin (CBDCA) were studies an 7,12-dimethylbenz (a) anthracene induced rat ovarian cancer cell line (DMBA-OC-1) and a human ovarian serous adenocarcinoma cell line (KOC-1S). The IC50 dosage of anticancer drugs for DMBA-OC-1 was: CDDP 0.2 microgram/ml. ADM 0.04 microgram/ml, VP-16 3.0 microgram/ml, MMC 0.1 microgram/ml and CBDCA 10.0 micrograms/ml. The results of our study except those for MMC were parallel with those published on in vivo studies. The IC50 dosages of DMBA-OC-1 did not have enough antitumor activity for KOC-1S, whereas the original KOC-1S tumor strongly resisted the combination chemotherapy (CDDP, ADM and cyclophosphamide). Therefore, our findings suggested the possibility of the separation of multiple drug resistant clones from KOC-1S. These two cell lines had quite different antitumor activity characteristics.

9,10-Dimethyl-1,2-benzanthracene↗