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Biomedical subjects

T Miyake

Publications and source records attributed to T Miyake.

At least 343 records · Page 19Linked to original sources
PubMed1986

Neuraminidase and hematopoietic factors from human urine.

Human urinary neuraminidase, an enzyme that releases sialic acid from hematopoietic factors found in urinary preparations, was partially characterized, and a method was developed to derive these hematopoietic factors free of enzyme activity. Neuraminidase in urinary preparations from healthy humans and aplastic anemic (AA) patients had optimal activity at pH 5.3 and hydrolyzed both alpha 2----3 and alpha 2----6 type ketosidic linkages of N-acetyl-neuramin lactose and alpha 1-acid glycoprotein. When subjected to Sephacryl S-300 gel filtration, urinary neuraminidase showed a single peak of activity with an apparent molecular weight of 380,000 daltons, even under denaturing conditions (6 M guanidine hydrochloride). Furthermore, among a variety of compounds tested, no potent inhibitor of the enzyme was found. Heat treatment of AA urinary preparations eliminated about 80% of neuraminidase activity, while successive two-step ethanol precipitation eliminated residual enzyme. Erythropoietin, megakaryocyte colony-stimulating factor (CSF) and granulocyte/macrophage phage CSF activities were retained after these treatments.

Anemia, Aplastic↗
PubMed1986

Biochemical properties of human urinary megakaryocyte colony-stimulating factor and erythropoietin: the role of sulfhydryl groups and disulfide bonds.

The labeling of cystine residues with [1-14C]iodoacetic acid showed that urinary preparations from patients with aplastic anemia contained 3.06 X 10(-9) mol of sulfhydryl groups and 2.90 X 10(-7) mol of half-cystine as disulfide bonds in the native state, and 6.36 X 10(-7) mol in the denatured state per absorbance unit of protein, respectively. Sulfhydryl reagent-treated proteins retained full activity of megakaryocyte colony-stimulating factor (Meg-CSF) and erythropoietin (Epo), except with DTNB-treated protein. Reduction-carboxymethylation and reduction-mercuration resulted in complete loss of Meg-CSF and Epo activities, suggesting that one of the essential chemical groups of Meg-CSF and Epo is a disulfide bond. Reduction of disulfide bonds at neutral pH revealed that Meg-CSF is less susceptible to reduction than Epo. Reactivation occurred by spontaneous reoxidation in most of the reduced Meg-CSF (92.6%) and part of the reduced Epo (22.1%). These molecular behaviors may reflect differences in the spatial configurations of Meg-CSF and Epo.

Colony-Stimulating Factors↗
PubMed1986

Agammaglobulinemia in a pregnant woman.

A case of common variable immunodeficiency in a pregnant woman is presented. Lobar pneumonia developed in the sixth month of the pregnancy and her serum immunoglobulin levels were found to be extremely low. She was treated successfully with immune human serum globulin and antibiotics. She delivered a full-term baby without any troubles. Various immunologic studies were done in the peripheral blood of the patient and the neonate. Consequently, functional abnormalities of helper T cells were considered to be responsible for the hypogammaglobulinemia.

Adult↗
PubMed1986

"Congestion index" of the portal vein.

The "congestion index" is used to mean the ratio between the cross-sectional area (cm2) and the blood flow velocity (cm/sec) of the portal vein, as determined by a duplex Doppler system. The indices as determined in normal subjects and patients with liver disease were as follows: normal subjects (n = 85), 0.070 +/- 0.029 cm X sec; acute hepatitis (n = 11), 0.071 +/- 0.014 cm X sec; chronic active hepatitis (n = 42) 0.119 +/- 0.084 cm X sec; cirrhosis (n = 72), 0.171 +/- 0.075 cm X sec; and idiopathic portal hypertension (n = 11), 0.180 +/- 0.107 cm X sec. There was a statistically significant difference between the congestion indices from the normal subject group and indices obtained from patients with chronic hepatitis, cirrhosis, and idiopathic portal hypertension. A weak positive correlation was obtained between the congestion index and the portal venous pressure, measured simultaneously through a percutaneously placed catheter (n = 64, r = 0.45, p less than 0.01). It is suggested that the congestion index reflects the pathophysiological hemodynamics of the portal venous system in portal hypertension.

Blood Flow Velocity↗
PubMed1986

[Adult T-cell leukemia and non-Hodgkin's lymphoma in brothers].

Adult T-cell leukemia/lymphoma (ATL) is an endemic disease in the Southwestern districts of Japan. Intrafamilial cluster of ATL and other T-malignancy frequently reported in these area. We reported cases of ATL and non-Hodgkin's lymphoma in brothers, they were born in Hokkaido where is non-endemic area of ATL. The first case (ATL) is 53 y. male and the second case (non-HD), younger brother of the first case, is 55 y male. Anti-ATLA (ATL-virus associated antigen) antibody were positive in both of them, their elder sister, wife and child of the first cases. Examination of anti-ATLA antibody and follow up study is necessary in the family members of ATL, because they may be in a high risk group of ATL outbreak.

Antibodies, Viral↗
PubMed1986

Hemopoietic colony formation by mouse spleen cells in serum-free culture supported by purified erythropoietin and/or interleukin-3.

We examined colony formation by mouse spleen cells in a serum-free culture system containing purified erythropoietin (Ep), interleukin-3 (IL-3) or a combination of the two factors. Purified Ep supported erythroid colony and burst formation but did not support formation of other types of colonies. IL-3 alone did not support erythroid burst formation in culture. However, IL-3 significantly increased the number and size of erythroid bursts. IL-3 but not Ep supported megakaryocyte colony formation. Ep did not enhance megakaryocyte colony formation or megakaryocyte populations in multilineage colonies supported by IL-3.

Animals↗