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Biomedical subjects

T Misaki

Publications and source records attributed to T Misaki.

At least 307 records · Page 17Linked to original sources
PubMed1983

Inhibition of thyrotropin-induced adenosine 3'5'-monophosphate increase by immunoglobulins from patients with primary myxedema.

Immunoglobulin G (IgG) fractions prepared from the serum of 18 patients with primary myxedema, 9 patients with goitrous Hashimoto's thyroiditis, and 14 normal controls were tested for their ability to alter TSH stimulation of cAMP production in cultured human thyroid cells and the binding of TSH to its receptor. When compared with the cAMP increase induced by 0.1 mU/ml bovine TSH in the presence of normal IgG, cAMP accumulation was significantly inhibited (P less than 0.005) by the addition of IgG from patients with primary myxedema. TSH-induced cAMP accumulation was not affected by IgG from patients with goitrous thyroiditis. IgG from patients with primary myxedema also inhibited the cAMP increase induced by thyroid-stimulating immunoglobulins, but not against the increase induced by prostaglandin E1. None of the IgG tested affected the basal level of cAMP. Two potent inhibitory IgG were strongly positive for TSH-binding inhibitor immunoglobulins. Excluding these, no significant correlation was found between the thyroid stimulation-blocking activity and the TSH-binding inhibitory activity. These data suggest the presence of at least two different types of antibodies in primary myxedema which block adenylate cyclase stimulation by TSH and might be responsible for thyroid dysfunction and atrophy.

Adenylyl Cyclase Inhibitors↗
PubMed1983

Partial purification and properties of the TSH receptors from human thyroid plasma membranes.

Human thyroid plasma membranes were solubilized with 0.5% Triton X-100 and TSH receptors were purified by using DEAE-Sephadex, Con A and TSH affinity chromatography. A TSH binding activity was bound to DEAE-Sephadex equilibrated with 0.05 M sodium acetate, pH 6.3, 0.2% Triton X-100 and was eluted by a linear gradient of 0.1 M to 1.0 M ammonium acetate, pH 6.3. Eighty-five percent of the activity was absorbed to Con A Sepharose and was eluted with 0.5 M alpha-methyl-D-mannoside, 0.05 M sodium acetate, pH 6.0. Seventy-five per cent of the TSH binding capacity could be absorbed to TSH-affinity column and was eluted with 0.1 M glycine-HCl, pH 3.0. By sequential application of the above procedures, more than 100-fold purification of the receptor activity was attainable. [125I]TSH binding of this fraction was inhibited by addition of unlabelled TSH in a dose-dependent manner. Scatchard analysis gave a curvilinear plot with a high affinity association constant of 0.72 X 10(9)M-1. By using Ultrogel AcA 34 gel filtration, the molecular size of the hormonereceptor complex was estimated to be 180 000.

Cell Membrane↗
PubMed1983

[Combination chemotherapy with adriamycin, carboquone and 5-Fu for bladder cancer].

We treated 25 bladder cancer patients with combined cytotoxic chemotherapy of adriamycin (10--20 mg/day administration on days 1, 2 and 3), carboquone (4 mg/day administration on day 1 of weeks 3, 4 and 5 and 5-Fu (200 mg/day for 5 weeks) or futraful suppositories (750 mg/day for 5 weeks) as one course. According to the Koyama - Saitoh criteria, CR + PR was observed in 4 (17.4%) of 23 patients excluding the 2 dropout patients. According to Karnofsky's criteria, an effect with chemotherapy was observed in 9 (39.1%) of the 23 patients. There was a relatively good response rate in a group of 12 patients with the superficial tumors as compared with a group of 11 patients with the deep tumors. There were no severe adverse reactions.

Aged↗
PubMed1983

[Renal tuberculosis with a contralateral renal adenocarcinoma: a case report].

A 62-year-old man, who complained of gross hematuria, was admitted because of a space occupying lesion of the right kidney and left non-visualizing kidney on IVP. The right pyelogram was compressed laterally, and the left pyelogram showed a moth-eaten appearance of the middle and lower calyces which was consistent with tuberculosis. Abdominal aortography revealed a hyperneovascularization over the middle and upper portions of the right kidney. CT-scan revealed a low density area of the right kidney and left atrophic kidney. No distant metastases were disclosed by bone scanning, liver scanning, lymphangiographic and chest X-ray studies. Bilateral nephrectomy was performed because the right renal tumor occupied the main renal artery. Hemodialysis was started on the next day. Postoperative course was uneventful. Histological examinations of the surgical specimen revealed a right renal adenocarcinoma and left renal tuberculosis. We discuss the pathogenesis and treatment of the combination of renal tuberculosis and malignant tumor.

Adenocarcinoma↗