Biomedical subjects
T Matuhasi
Publications and source records attributed to T Matuhasi.
The in vivo antibody responses to polymerized flagellin (POL) in man.
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Specificity of an anti-murine B cell serum.
An antiserum (ABS) specific for murine B cells was prepared in rabbits by immunization with lymph node cells from nude mice as reported previously (1). To make the antiserum specific for antibody-forming cell precursors (AFCP) extensive absorption was required with mouse serum, erythrocytes and thymus cells. Even after ABS was no longer cytotoxic for thymus cells, further absorption with thymus cells was necessary to make it ineffective for plaque-forming cells (PFC). The correlation between cells bearing C3- or Fc-receptors and the cells bearing antigen(s) to which ABS reacts was studied. In the residual spleen cells treated with ABS and complement, there were some EAg and EAmCm rosette-forming cells. There were also some spleen cells reacting with ABS even after depletion of C3- and/or Fc-receptor-bearing cells. These results show that the antigen(s) to which ABS reacts could be a marker on B cells different from C3- or Fc-receptors. Spleen cells reacting with ABS were compared with cells bearing C3- or Fc-receptors various days after birth. The cells sensitive to ABS increased in spleens earlier after birth than those with C3- or Fc-receptors.
Antigen specific receptors on murine B cell lineage. 1. On antibody forming cells in various stages of immune response.
In this study it was examined whether or not antigen specific receptors are on direct and indirect plaque-forming cells (PFC). Spleen cells from mice primed both with horse red blood cells (HRBC) and sheep red blood cells (SRBC) were incubated with HRBC to make rosettes and fractionated on Ficoll-Hypaque density gradient. Both direct and indirect HRBC-PFC were depleted from the interface fraction. The depletion was antigen specific and inhibited by the preincubation of spleen cells with antiserum against kappa- or mu-chain for direct PFC and with antiserum against kappa- or gamma 1-chain for indirect PFC. The depletion was not due to the rosette formation mediated by Fc-receptors which might be on antibody forming cells, because PFC were not eliminated by the density gradient sedimentation after incubation of spleen cells with SRBC coated with anti-SRBC IgG antibody in our experimental conditions. Our results show that the antigen specific immunoglobulin receptors are on the cells producing IgG antibody after primary or secondary immunization as well as on the IgM antibody-forming cells even in late stage of immune response.
Antigen specific receptors on murine B cell lineage. 2. Different effect of rosette-forming cell depletion on IgM and IgG antibody forming cell precursors in primary and secondary response.
Spleen cells from unprimed mice or those primed with horse red blood cells (HRBC) were depleted of rosette-forming cells (RFC) with HRBC by the Ficoll-Hypaque density sedimentation, and the cells were examined in the adoptive transfer system whether they could raise IgM or IgG antibody-forming cells (AFC) after an immunization with HRBC. When spleen cells were pooled from unprimed mice, the response to HRBC of those depleted of RFC with HRBC (HRBC-RFC) was decreased to about a half in both IgM and IgG AFC. On the other hand, when spleen cells were from mice primed with HRBC, the response to HRBC of those depleted of HRBC-RFC was decreased dramatically to 1/20 of that of original cells in IgG AFC, but it was decreased to about a half in IgM AFC. In the time course of the response to HRBC of RFC-depleted spleen cells from mice primed with HRBC, an early IgG response was abolished but the late one was as high as that of untreated spleen cells. These results suggest that the depletion of RFC is most effective on the depletion of direct precursors of the secondary IgG AFC.
[Induction of Antigen Specific and IgE selective suppression of antibody response (author's transl)].
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Effect of lipopolysaccharide (LPS) on the thymocyte response to PHA: strain difference.
Thymocytes responded well to PHA in terms of [3H]-TdR incorporation if they were precultured in the presence of LPS, whereas fresh thymocytes or thymocytes pre-cultured in the absence of LPS responded poorly to PHA. The PHA response of thymocytes pre-cultured with LPS for several hours was similar to that of fresh spleen cells in the kinetics and dose-response profiles. The effect of LPS was found on thymocytes from BALB/c.Cr, AKR/Jms, and DDD/1 mice, but was not observed on those from C3H/HeJms and C57Bl/6J mice, indicating that there is a strain difference in the PHA responsiveness of LPS-pre-cultured thymocytes. In contrast to thymocytes, the response of spleen cells to PHA was enhanced in both C57Bl/6J and BALB/c.Cr mice by pre-culture with LPS.
Analyses of some mite antigens found in house dust and in food material.
Antigen analyses by micro-Ouchterlony, immunoelectrophoresis and passive hemagglutination tests were carried out on extracts from each of the following cultured mites: Dermatophogoides farinae, Dermatophogoides pteronyssinus, Tyrophagus putrescentiae, Aleuroglyphus ovatus and Carpoglyphus lactis; and on an extract from the culture medium for each mite. Antisera were raised against each extract or each medium in rabbits or in guinea pigs. Up to 5 precipitin lines by micro-Ouchterlony test and up to 8 precipitin arcs by immunoelectrophoresis were detected between an extract and its homologous (corresponding) antiserum. Some of the lines or arcs were seen consistently to be common to all species tested. The absorption of common antigens in an extract by heterologous (noncorresponding) antiserum was employed to find the antigens specific to D. farinae and D. pteronyssinus. By these tests, at least 3 species-specific antigens for D. farinae and 2 for D. pteronyssinus were found. In passive hemagglutination tests, higher titers were obtained mainly in homologous systems, although weak cross reaction was observed in every system. Culture media did not participate in any reaction combinations used here and did not interfere with the analyses.
Detection by proton nuclear magnetic resonance of elevated lactate concentration in serums from patients with malignant tumors.
Proton nuclear magnetic resonance (1H-NMR) spectra of the serum specimens from patients with malignant tumors were compared with those from presumably healthy persons. We found that 87% of serum specimens from the patients yielded a common proton signal, which was ascribed to the methyl protons of lactic acid; whereas only 9% of serum specimens from the healthy persons tested gave this signal. On the basis of these results we concluded that the lactate level in the serum can be used as a criterion for the diagnosis of cancer in humans and that the determination of lactate concentration in the serum is easily performed by means of 1H-NMR.
[Comparative evaluation study on micro-TPHA reagents (author's transl)].
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IgE-selective and antigen-specific unresponsiveness in mice. I. Induction of the unresponsiveness by administration of ovalbumin-pullulan conjugate.
An experimental model was demonstrated in mice for the induction of IgE-selective unresponsiveness to ovalbumin, a protein antigen. An administration of ovalbumin, conjugated with pullulan, a linear polymer of glucose, (OA-pullulan) into mice resulted in the induction of a long lasting, IgE-selective unresponsiveness to the subsequent immunization with native OA in the form optimal to elicit IgE antibody response. The IgE-selective unresponsiveness is antigen specific and is infectious to normal mice by transferring the spleen cells from mice receiving OA-pullulan conjugate at least 2 weeks before. In contrast to other modified antigens, OA-pullulan was found to elicit good IgM and IgG antibody responses, but not an IgE response, without the aid of an adjuvant.
IgE antibody production to exoenzymes and common antigen (OEP) of Pseudomonas aeruginosa in mice.
IgE antibody production to exoenzymes and common antigen (OEP) of P. aeruginosa was studied in mice that were injected with the antigens incorporated into water-in-oil-in-water (w/o/w) emulsion or Al(OH)3 gel. OEP and protease toxoid (PT) elicited IgE antibody response but elastase toxoid (ET) did not. Capacity of the OEP to produce IgE antibody was reduced remarkably by it's protease treatment, which suggested that the capacity lies in the protein portion of OEP. The w/o/w emulsion was less effective than Al(OH)3 gel in adjuvanticity to elicit IgE antibody response, but the emulsion enhanced the IgM and/or IgG antibody response to PT and maintained a constant level for a long period. These findings may suggest that IgE antibody response to some components of P. aeruginosa could be induced in man. High serum level of IgE was observed in some cases of cystic fibrosis caused by P. aeruginosa infection, although the IgE antibody activity has not yet been determined.
Studies on the adjuvant effect of water-in-oil-in-water (w/o/w) emulsion of sesame oil. 2. Mode of action of the w/o/w emulsion.
The water-in-oil-in-water (w/o/w) emulsion showed potent adjuvant effect on the antibody formation to thymus-dependent antigens, but not to thymus-independent antigens. In hapten-carrier system the priming with carrier in the w/o/w emulsion enhanced more effectively the carrier specific helper function than did the priming with carrier in free solution. The cells responsible for the helper function were radioresistant. In the adoptive cell transfer system, the w/o/w emulsion was shown to enhance helper cell function. It is discussed that our w/o/w emulsion exerts the adjuvant effect by enhancing helper T cell activity.
Studies on the adjuvant effect of water-in-oil-in-water (w/o/w) emulsion of sesame oil. 1. Enhanced and persistent antibody formation by antigen incorporated into the water-in-oil-in-water emulsion.
Water-in-oil-in-water (w/o/w) emulsion developed in our laboratory is as effective as water-in-oil (w/o) emulsion of Freund's incomplete adjuvant (FIA) in the stimulation of antibody formation. The emulsion is prepared by redispersion of water-in-sesame oil emulsion of an antigen solution in phosphate buffered saline with emulsifier, Tween 80. The emulsion can be stored at 4 degrees C for at least 3 months without any evidence of change in the adjuvanticity and in the w/o/w state. Even a single injection of bovine serum albumin (BSA) in the w/o/w emulsion elicited a high antibody response in mice over the period of almost whole lifespan. 10 microgram BSA in w/o/w could stimulate antibody formation up to 2(12) in hemagglutination titer, while the same dose in free solution did not elicit any detectable antibody. The tissue reactions caused by the w/o/w emulsion at the injected site and in the regional lymph nodes were much less prominent than those by FIA.
Demonstration of two types of helper T cells for different IgG subclass responses to dinitrophenylated flagellin polymer.
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Immunological studies on CS-1170, a new semisynthetic cephamycin antibiotic.
The antigencity of CS-1170, a newly developed semi-synthetic cephamycin, and it's cross-reactivity with beta-lactam antibiotics were studied. The antigencity was confirmed by the following tests: 1)the passive hemagglutination test with anti-CS-1170 antisera of guinea pigs immunized with CS-1170 plus Freund complete adjuvant, 2)lymphocyte proliferation response in vitro stimulated with this drug in the guinea pigs, 3) the PCA test using mice immunized with CS-1170-protein conjugates. The antibody to CS-1170 appears to be directed to the acyl side chain because cyanomethylthioacetylglycine, an univalent acyl side chain of the CS-1170, can significantly cross-react with the antibody. A methoxy group on the C-7 alpha-position of the antibiotic plays no important part for the antigenic specificity of the molecule. The cross-reactivity of CS-1170 with cefazolin, cephalothin, benzylpenicillin and ampicillin was only to a minimal extent in the passive hemagglutination test. The cross-reactivity of CS-1170 and the related antibiotics was not observed among them in the PCA system with IgE anti-CS-1170. These findings support a conclusion that CS-1170 is one of beta-lactam antibiotic with an immunologically minimal cross-reactivity to the related antibiotics.
The modification of immunological development of mice by maternal alloantibody.
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Nonspecific binding of irrelevant antibodies to the complexes of proteinic antigen and its antibody.
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