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Biomedical subjects

T Matuhasi

Publications and source records attributed to T Matuhasi.

At least 37 records · Page 2Linked to original sources
PubMed1986

Immunopotentiative effect of polysaccharide from kefir grain, KGF-C, administered orally in mice.

Since a water-soluble polysaccharide (KGF-C) from the kefir grains was shown to have the property of retarding tumor growth in vivo when administered orally, the effect of KGF-C was examined on antibody responses to thymus-dependent antigen, sheep red blood cells (SRBC), and thymus-independent antigen, dinitrophenyl-Ficoll and trinitrophenyl-lipopolysaccharide. Antibody response in mice intubated with KGF-C was enhanced to low doses of SRBC, but not to optimal or high doses. The optimal dose of KGF-C required for the enhancement was 100 mg/kg body weight. The time-course studies on KGF-C administration implied that KGF-C exerted its effect on the early events of anti-SRBC response. The enhancement was not due to the alteration of kinetics of anti-SRBC responses. Furthermore, the enhancing effect on antibody responses to thymus-independent antigens, such as dinitrophenyl-Ficoll and trinitrophenyl-lipopolysaccharide, was observed neither in nu/nu nor in nu/+ mice, and the effect on delayed-type hypersensitivity response to a low dose of SRBC in normal mice was also found. These findings suggest that the oral immune enhancement by KGF-C is elucidated probably through T-cell but not through B-cell participation.

Adjuvants, Immunologic↗
PubMed1985

IgE antibody against hepatitis B surface antigen in mice.

Hepatitis B surface antigen (HBs antigen) was examined for elicitation of IgE production by injection into mice. The Prausnitz-Küstner (PK)-type skin test in the rat was employed for detection of IgE antibody to HBs antigen, because no sufficient purified HBs antigen was available as the challenging antigen for the passive cutaneous anaphylaxis (PCA) test in rats. The positive PK test was considered to be due to IgE antibody, since the active principle was inactivated by heating the sera at 56 C for 30 min, did not bind to protein A and was eliminated by anti-mouse IgE antisera. These data indicate that the PK-type test in rats can be used for detection of mouse IgE antibody when the amount of a test sample is not sufficient for the PCA test in rats.

Adjuvants, Immunologic↗
PubMed1983

Microcapsule agglutination test for Treponema pallidum antibodies. A new serodiagnostic test for syphilis.

For the serodiagnosis of syphilis a quantitative passive agglutination (MCA-TP) test for antibodies to Treponema pallidum was performed with chemically stable microcapsules with no antigenic activity instead of with conventional sheep erythrocytes. The microcapsules were easily sensitised with the antigen of sonicated Treponema pallidum by treatment with glutaraldehyde. Compared with the Treponema pallidum haemagglutination test (TPHA) the MCA-TP test was superior for detecting cases of primary syphilis. Furthermore, the decrease in antibody titre during treatment was more evident in this test than in the FTA-ABS or the TPHA tests. The MCA-TP test performed on IgM and IgG gel-filtered fractions of sera from patients with syphilis proved that the sensitised microcapsule antigen reacted sharply with the IgM antibodies specific to syphilis.

Agglutination Tests↗
PubMed1983

Detection and characterization of polymers in cephalothin by passive cutaneous anaphylaxis in mice.

Immunization of BALB/c mice by repeated injections of cephalothin (CET) - Ascaris suum extract conjugate resulted in formation of IgE antibodies, which were able to sensitize syngeneic animals for passive cutaneous anaphylaxis (PCA). By means of high-pressure liquid chromatography, a fraction with extremely high PCA-eliciting activity, but without appreciable antimicrobial activity, was isolated from the CET preparation. Physicochemical analyses of the fraction identified the major component of polymer impurities as being a proteinaceous complex with a molecular weight of 6,580. Very little cross-reactivity of CET and potassium benzyl penicillin (PcG) was noted when these antibiotics were used as the challenge antigens for PCA induced by corresponding murine antisera. The results of the inhibition studies indicated, however, that at least two antigens were involved in the PCA induced by anti-CET antibodies, one strictly specific for CET and another shared by PcG. Evidence was also presented that the nucleus structure and acyl side-chain structure of CET play the major role in the PCA elicited by the challenge with CET and its polymer, respectively.

Animals↗
PubMed1982

Immunoglobulin E-suppressing and immunoglobulin G-enhancing tetanus toxoid prepared by conjugation with pullulan.

Immunoglobulin E (IgE) antibody response was found to be suppressed selectively and antigen specifically in mice given an antigen conjugated with pullulan, a linear copolymer of maltotriose, whereas IgM and IgG antibody responses were enhanced. On the basis of this finding, tetanus toxin was conjugated with pullulan by cyanuric chloride in the hope that the toxin would be detoxified by the conjugation procedure and could be used as an IgE-suppressing and IgG-enhancing toxoid without the aid of an aluminum adjuvant. This procedure of tetanus toxoid-pullulan conjugation apparently detoxified the toxin. Administration of the resulting tetanus toxoid, tetanus toxin-pullulan conjugate, to mice induced strong suppression of IgE antibody response with fairly good IgG response, whereas the alum-precipitated toxoid or plain toxoid, customarily used for vaccination, elicited high IgE antibody formation. The IgE antibody response was minimal, but the IgG antibody response was maximal in the conjugate-primed mice even after a booster injection with an IgE antibody-inducing dose of the alum-precipitated toxoid.

Animals↗
PubMed1982

Development of a simple serological method for diagnosing leptospirosis: a microcapsule agglutination test.

A passive microcapsule agglutination test for the diagnosis of leptospirosis was developed by utilizing chemically stable microcapsules instead of sheep erythrocytes. In the test, sonically disrupted antigens of leptospira were sensitized to microcapsules treated with glutaraldehyde. Compared with the microscopic agglutination test, the passive microcapsule agglutination test showed a relatively genus-specific tendency and a 4- to 32-fold-higher sensitivity. The sensitized microcapsule antigens were stable for at least 1 year. The microcapsules coupled with mixed antigens can be used as a serodiagnostic screening test for diseases caused by various types of leptospira. The test, which is very simple and reproducible and requiring no specific training, can be employed easily as a routine test in diagnostic laboratories.

Agglutination Tests↗
PubMed1982

Antigenicity of beta-lactam antibiotic preparations: production of IgE antibodies to beta-lactam antibiotic and their cross-reaction within the antibiotic group.

BALB/C mice were immunized with conjugate of benzylpenicillin or ampicillin with Ascaris suum extract. The mice developed IgE antibodies to penicillin, which were found to react with commercially available penicillin preparations in the PCA system. Elimination of polymerized penicillin by Sephadex chromatography from tested preparations could not diminish their activities to elicit PCA. High pressure liquid chromatographic (HPLC) separation of PcG preparation yielded fractions unable to elicit PCA. On the other hand, all fractions from ABPC retained the activity even after the HPLC purification. A comparative estimation of cross-reactivity of IgE and IgE antipenicillin antibodies showed that IgE antibodies cross-reacted with a variety of beta-lactam antibiotics in a high degree.

Ampicillin↗
PubMed1982

Different effect of LPS-induced macrophage factor on antibody responses to TI-1 and TI-2 antigens.

Effect of macrophage culture fluid (MF) on thymic independent (TI) antibody responses was examined. MF potentiated antibody responses of spleen cells to dinitrophenyl (DNP)-Ficoll and DNP-liposome, TI-2 antigens, but not to trinitrophenyl (TNP)-BA and TNP-LPS, TI-1 antigens. The enhancing effect of MF on the anti-DNP-Ficoll response was dose-dependent. Neither T cells nor macrophages were required for MF to exert the effect, suggesting that MF works on B cells directly. B cells modulated by MF in their antibody responses were indicated to be in mature B-cell subset for the following reasons: (i) the cells were in (CBA/N x BALB/c) F1 female but not in F1 male mice; (ii) the cells bore the receptors for C3 on their surface. MF was indicated to exert the enhancing effect on the antibody response by modulating the proliferation and/or early events in differentiation of B cells and not by promoting antibody secretion. The active component of the MF was indicated to be Interleukin 1.

Animals↗