Search PubMed⌕ Search

Biomedical subjects

T Kasai

Publications and source records attributed to T Kasai.

At least 289 records · Page 16Linked to original sources
PubMed1986

[Experimental studies on photoradiation therapy (PRT)--effects of PRT on cultured human cancer cell lines].

Hematoporphyrin derivative (HpD) is known to have an affinity for tumor cells. Photoradiation therapy (PRT), in which HpD is activated by an argon dye laser, is not always effective in treating all gynecological malignancies. Fundamental studies were performed to elucidate the mechanism acting on two cell lines and to evaluate efficiency when applied to them. Two cell lines established from endometrial adenocarcinoma and uterine choriocarcinoma were used in this study. There were apparent differences in 3H-thymidine incorporation on two cell lines treated by single HpD. In both cell lines, single HpD has some effects on neutral red uptake depending on the HpD concentration and length of treatment time. In PRT, a proportional increase in effects is obtained by extending the irradiation time. More than 90% effectiveness is obtained by pretreatment with HpD 40 micrograms/ml and irradiation for 3 minutes or more. On the DNA patterns of the cells, a shift in the histogram peak to diploid and a remarkable decrease in the number of high ploidy cells above tetraploidy occurred but were not so noteworthy in these two cell lines as in SKG-1 cells.

Adenocarcinoma↗
PubMed1985

In-vitro and in-vivo activities of a novel cephamycin MT-141 against the Bacteroides fragilis group in comparison with six cephem antibiotics.

MT-141 inhibited 90% of the clinical isolates of Bacteroides fragilis at 3.13 mg/l, whereas six reference cephem antibiotics inhibited them at 12.5- greater than 100 mg/l. MT-141 was stable to the Bacteroides beta-lactamases, but the stability alone could not explain the potent activity of MT-141 against the other Bacteroides spp., which produced only small amounts of beta-lactamase, but were resistant to other cephem antibiotics. MT-141 exhibited a higher killing rate and more potent in-vivo efficacy than the reference cephem antibiotics against Bact. fragilis No. 36 combined with Escherichia coli No. 29. In conclusion, the in-vitro and in-vivo anti-bacteroides activities of MT-141 ranked first among those of the cephem antibiotics compared.

Animals↗
PubMed1984

Influence of arm positions on EMG-reaction time of the biceps brachii for elbow flexion and forearm supination.

The influence of starting positions of the arm on EMG-RTs of the biceps brachii muscle for elbow flexion and forearm supination was examined using 16 normal subjects. Two angles of the elbow joint, 45 degrees and 110 degrees flexion, and two positions of the forearm, 45 degrees supination and 90 degrees pronation, were used as the factorial combinations of all four. The EMG-RT for elbow flexion decreased in the order of 110 degrees Pronation greater than 45 degrees Pronation = 110 degrees Supination greater than 45 degrees Supination, and that for forearm supination decreased in the order of 45 degrees Supination greater than 45 degrees Pronation = 110 degrees Supination greater than 110 degrees Pronation. These results were kinesiologically interpreted that variations of EMG-RTs were based on the change in the number of synergic muscles participating in an intended movement and the muscle length of the prime mover at the start of the movement.

Adolescent↗
PubMed1984

[Antibacterial activity of MT-141 against anaerobic bacteria].

In vitro and in vivo antibacterial activities of MT-141, a new cephamycin, against anaerobic bacteria were compared with those of cefmetazole (CMZ), cefoxitin, cefotaxime, ceftizoxime, latamoxef and cefazolin to obtain the following results. MT-141 showed high activity against a wide variety of anaerobic bacteria including B. fragilis and C. difficile except for Eubacterium lentum, E. aerofaciens and B. furcosus. Antibacterial activity of MT-141 against anaerobic bacteria was almost independent of inoculum size, medium, pH and serum addition. In vitro development of resistance of MT-141 against P. variabilis and B. fragilis was comparable to that of CMZ. Successive parenteral administration of MT-141 into mice did not cause abnormal proliferation of C. difficile. MT-141 showed excellent therapeutic effect against experimental subcutaneous abscess in mice caused by B. fragilis.

Animals↗
PubMed1984

[Experimental studies on photoradiation therapy by hematoporphyrin derivative (HpD) and argon dye laser].

A light sensitive substance, Hematoporphyrin Derivatives (HpD) is known to have an affinity with tumor cells, which are degenerated to necrosis by photoradiation therapy (PRT). Fundamental studies were performed to elucidate the mechanism acting on tumor cells and to evaluate efficiency when applied to gynecological malignancies. The SKG-1 cell line established from uterine cervical cancer was used in this study. No effects on cell growth inhibition (CGI) can be expected by single irradiation. Single HpD has some effects on CGI depending on the HpD concentration and length of treatment time. In PRT, a proportional increase in effects is obtained by extending the irradiation time. More than 90% effectiveness is obtained by irradiation for 5 minutes or more. In a cytomorphological study of the time course changes in cultivated cells subjected to PRT, prominent cytoplasmic changes are observed even immediately after PRT. Advanced changes in nuclei are seen within a few hours after PRT. Electron microscopic findings are mitochondrial swelling and destruction, ER distension, destruction of plasmamembrane and decreased density of nuclei as chromatin disappears. The DNA pattern of the cells 24 hours after PRT showed a shift in the histogram peak to diploid and a remarkable decrease in the number of high ploidy cells above tetraploidy. This indicates early degeneration of DNA after PRT.

Carcinoma, Squamous Cell↗
PubMed1984

[Analytical diagnosis in colposcopy by subdivision of abnormal findings].

Abnormal findings of Colposcopy (Colp) were classified in contrast with histology. Varieties of colposcopic abnormal findings appear in more advanced changes in cervical diseases; single in squamous metaplasia, dysplasia and some Ca in situ, double in Ca in situ and microinvasive Ca, triple microinvasive and advanced carcinoma. Atypical vessels are frequently encountered in invasive carcinoma. Circular extension of abnormal figures around external os indicated the following correlation: Within 1/4-2/4 in dysplasia, 2/4-3/4 in Ca in situ and microinvasive Ca, 3/4-4/4 in microinvasive and invasive Ca. In squamous metaplasia and dysplasia, the white epithelia were flat on the surface, comparatively clear in the white part and less elevated. On the other hand, in carcinoma in situ, corresponding to the pathological area, they were rough on the surface, unclear in the white part and highly elevated. Compared with squamous metaplasia and dysplasia, carcinoma in situ in punctation revealed more surface elevation and unclear white part. Characteristic histological figures are dot types in the metaplasia and dysplasia, nest-like types in the carcinoma in situ and atypical vessel transition types in the microinvasive carcinoma. Complete types of mosaic were observed more in metaplasia, slight dysplasia and carcinoma in situ, while the incomplete types were common in moderate and severe dysplasia. Microinvasive carcinoma mosaic revealed more incomplete types and atypical vessel transition types. As for surface elevation and unclear white part, the same tendency was noticed as in punctation and white epithelium.(ABSTRACT TRUNCATED AT 250 WORDS)

Carcinoma, Squamous Cell↗
PubMed1984

[Studies on the early diagnosis of endometrial carcinoma. Analysis of risk factors and cytological approach].

Clinical, cytological and hormonal studies were performed on 86 cases of endometrial carcinoma and 34 cases of endometrial hyperplasia for their early diagnosis. 68.6% of the endometrial carcinomas were in patients aged 50 to 64 and 3.5% in patients under 45 ages. The figures 17.4% infertility, 20.9% no delivery and 19.8% only one delivery indicate that endometrial carcinoma is associated with no birth or the birth of few child. Almost all cases of endometrial carcinoma had existed for more than ten years since the last pregnancy, when the lesions were detected. 20.9% of endometrial carcinomas belonged to premenopause and 24.4% to within the first 5 years following menopause. In total, 45.3% of them range around menopause and hormonal imbalance in climacteric periods. Clinical stages of endometrial carcinoma revealed no relation to enlargement of the uterus. 61.4% of endometrial carcinoma were found in obese patients. 63.1% of them showed abnormal glucose tolerance titers and preclinical lesions. Hypertension was found in 28.0% of them, but we did not consider it very significant, considering their advanced age. The serum steroid level indicated no hyperestrogenism in endometrial carcinoma. Diagnostic data showing positive and suspecious smears in endometrial carcinoma were 36.5% in vaginal, 67.5% in cervical and 84.3% in endometrial cytology. This means that direct sampling of cells from the uterine cavity is essential in the detection of endometrial carcinoma. The cytological features of endometrial carcinoma were nuclear enlargement, anisokaryosis, irregular distribution of chromatin and prominent nucleoli. Undifferentiated types of endometrial carcinoma were more characterized by these factors than differentiated types.

Adult↗
PubMed1983

Lipid storage myopathy associated with scoliosis and multiple joint contractures.

Two patients with cleft palate, clubfoot, congenital scoliosis, and arthrogryposis multiplex congenita showed increased numbers of lipid droplets in biopsied muscle fibers. There was little abnormality in the mitochondrial morphology of the muscle and the results of blood chemistry including carnitine concentrations. Accumulation of large lipid droplets, increased amount of glycogen and a decreased number of mitochondria were observed in chondrocytes from the biopsied iliac crest cartilage of these patients. It was suggested that the present cases might belong to a previously unknown clinico-pathological entity of myopathies.

Arthrogryposis↗
PubMed1983

[Treatment of recurrent cervical cancer].

The questionnaire about recurrent cervical cancer of uterus were taken in 57 hospitals and treatments to 370 recurrent cases in National Institute of Radiological Science were studied. Our results were as follows. 1) About 50% of recurrent cases were detected within one year after primary treatments. Local recurrents were superior in cases of surgery as initial treatment, distant metastasis in cases of radiation. 2) Five Y.S.R. of 370 cases was 17.3% that of 105 operation cases as initial treatment was 26.7%. 3) Five Y.S.R. according to the sites of recurrence were 26.5% in vaginal stump, 6.7% in intrapelvis, 2.2% in lung metastasis, 5.5% in bone metastasis. 4) Five Y.S.R. according to the secondary treatments were 30% in operation, 16.4% in radiation, 6.9% in chemotherapy. 5) The principals of combined therapy to recurrent cervical cancer were presented.

Brachytherapy↗
PubMed1982

Effect of malotilate (diisopropyl 1,3-dithiol-2-ylidenemalonate) on the protein synthesis in rat liver.

The effect of malotilate (diisopropyl 1,3-dithiol-2-ylidenemalonate) on the protein synthesis in rat liver was studied in vivo and in vitro. Oral administration of malotilate to rats caused an increase in the protein and RNA contents of the liver and lef to an acceleration of 14C-leucine incorporation into microsomal and cytosol proteins. In a cell-free system, the protein synthesis was enhanced by treatment with malotilate, and an unknown factor(s) which participates in the protein synthesis was found in the cytosol fraction prepared from malotilate treated livers. These results suggest that malotilate is a new type of inducer for protein synthesis. On the basis of the observations obtained in the present study, a hypothesis can be formulated that malotilate enhances liver protein synthesis by accelerating RNA synthesis and/or increasing the transport of RNA from nuclei to cytosol in rat liver.

Animals↗