Search PubMed⌕ Search

Biomedical subjects

T Enomoto

Publications and source records attributed to T Enomoto.

At least 361 records · Page 20Linked to original sources
PubMed1978

Exposure of DNA bases induced by the interaction of DNA and calf thymus DNA helix-destabilizing protein.

The reaction of chloroacetaldehyde with adenine bases in DNA to give a fluorescent product was used to study the availability to intermolecular reaction of positions 1 and 6 of adenine in DNA complexes with calf thymus DNA helix-destabilizing protein. No inhibition of this reaction was observed when heat-denatured DNA was complexed with the protein at a protein/DNA weight ratio of 10:1, compared to free DNA. On the contrary, the same reaction was inhibited markedly for denatured DNA in the presence of calf thymus histone HI at protein/DNA weight ratio of 2:1. Furthermore, the exchange rate for hydrogens of amino and imide groups of DNA bases in DNA strands with deuterium in the solvent was totally unaffected upon complexing of DNA with the DNA helix-destabilizing protein as examined by stopped-flow ultraviolet spectroscopy. These results indicate that the DNA helix-destabilizing protein forms a complex with single-stranded DNA, leaving DNA bases uncovered by the protein. The fluorescence intensity of DNA pretreated with chloroacetaldehyde was amplified by nearly 3-fold upon addition of the DNA helix-destabilizing protein. The possibility of "unstacking" of DNA bases induced by the protein is discussed.

Acetaldehyde↗
PubMed1977

Further evidence of the presence of progesterone-binding proteins in female rat hypothalamus.

Utilizing techniques of Sephadex G-200 column chromatography, sucrose density gradient ultracentrifugation and dextran-coated charcoal adsorption, the characterization of progesterone-binding proteins was attempted in cytosols obtained from the medial basal hypothalamus including the median eminence, the remaining hypothalamus and the cerebral cortex of ovariectomized estrogen-primed rats. Ater incubation of the medial basal hypothalamic cytosol with 3H-progesterone in the absence or in the presence of various unlabeled steroids, a specific progesterone-binding protein was found in the 7 S region with its binding capacity of approximately 2 X 10(14) moles per mg protein and dissociation constant of approximately 6 X 10(-9) M. No specific binding was, however, found in the remaining hypothalamus and the cerebral cortex.

Animals↗