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Biomedical subjects

S Ueda

Publications and source records attributed to S Ueda.

At least 1,081 records · Page 60Linked to original sources
PubMed1981

Temperature-sensitive alteration in fusion activity of subacute sclerosing panencephalitis virus during serial passages in vitro and expression of hemagglutinin on the infected cells.

The Biken strain of subacute sclerosing panencephalitis (SSPE) virus, a maturation-defective variant of measles virus, was serially passed in human embryonic lung (HEL) cells at 37 C. The strain formed syncytial giant cells (GC) at both 37 C and 39 C, but the surface of infected cells did not show hemadsorption at early passages of the strain. However, GC-forming activity of the strain diminished at 39 C after 25 passages or more and hemadsorption on the infected cells became positive at both 37 C and 39 C after 40 passages or more of the strain. Hardly any infectious cell-free virus was detected in the culture fluid even after hemadsorption became positive. Possible mechanisms for the defect of SSPE virus were discussed.

Cell Fusion↗
PubMed1981

Changes in vascular reactivity in rats with experimental renal insufficiency.

In ureter-ligated rats, the pressor effects of both norepinephrine and angiotensin II i.v. on systemic blood pressure and serum sodium concentration were significantly decreased when compared with those in control rats, while serum potassium concentration, blood urea nitrogen, mean systemic blood pressure and plasma renin activity were significantly increased. The microscopic examination of aortic smooth muscles did not show significant histological lesions in the tissues obtained from ureter-ligated rat. The responsiveness of aortic strips isolated from ureter-ligated rats to norepinephrine and angiotensin II was significantly decreased in comparison with that from control rats. The results suggest that a decreased pressor response to norepinephrine and angiotensin II under ureter-ligated conditions may be attributed to a decrease in vascular reactivity to drugs.

Angiotensin II↗
PubMed1981

An extremely attenuated, live measles virus vaccine (CAM-EX). Persistence of low titer-antibody in institutionalized children with psychomotor retardation after vaccination.

Fifty children with psychomotor retardation, who had no detectable measles hemagglutination-inhibition (HI) antibody, were vaccinated with an extremely attenuated, live measles virus vaccine (CAM-EX). Only 10% of the children had fever after vaccination. No other symptoms were observed. Forty of 44 vaccinated children showed increase in HI antibody titers of 2(3) or more, with a geometric mean titer of 2(4.2). A five-year follow-up study revealed that the initially low-titer antibodies persisted well under conditions without a booster response due to natural measles.

Adolescent↗
PubMed1981

Application of live attenuated measles and mumps vaccines in children with acute leukemia.

Eight children with acute leukemia in remission were immunized with live attenuated measles vaccine and 4 with mumps vaccine. Immunological examinations before vaccination showed that the numbers of white blood cells, percentages of lymphocytes, levels of immunoglobulins and responses to skin tests with DNCB, PPD, PHA and varicella antigen were within normal ranges in most of these children. Chemotherapy against leukemia was stopped for 2 weeks, from one week before to one week after vaccination. One child had a transient fever 10 days after measles vaccination, but no side reactions were observed in the others. Seroconversion was observed in all but one child who received measles vaccine, and neutralizing antibodies have persisted for at least 4 weeks and at most 3 years, when this study was terminated.

Acute Disease↗
PubMed1981

Prevention of syngeneic tumor growth in vaccinia virus-primed mice by immunization with vaccinia virus-modulated tumor cells.

Immunization with vaccinia virus-infected and then X-ray-irradiated murine hepatoma MH134 cells provided C3H/He mice with strong resistance to challenge with viable MH134 cells. Male C3H/He mice of 5 to 6 weeks old were primed intraperitoneally (IP) with 1 x 10(7) PFU of live vaccinia virus (Ikeda strain) after irradiation with 250 R of X-ray. Three weeks after priming, the mice were immunized IP 3 times at weekly intervals with 1 x 10(7) X-ray-irradiated MH134 cells that had been infected with vaccinia virus 8 h before irradiation. Over 60% of these cells showed vaccinia virus-induced antigen on their surface (membrane antigen). Challenge with viable MH134 cells was done by inoculating 1 x 10(5) cells IP one week after immunization. During a 4-week observation period, all the untreated control mice died with ascites. On the contrary, all the mice that were X-ray-irradiated, primed and immunized survived challenge with the tumor cells for at least 4 weeks. The mortalities of mice in other groups that were not irradiated, or not primed, or immunized with only X-ray-irradiated tumor cells, were at lowest 50%.

Animals↗
PubMed1980

Endoscopic evaluation of the effect of sucralfate therapy and other clinical parameters on the recurrence rate of gastric ulcers.

A randomized controlled clinical study was conducted to determine (a) whether basic aluminum sucrose sulfate (sucralfate) is effective in preventing or reducing gastric ulcer recurrence, and (b) the clinical parameters contributing to ulcer recurrence after endoscopically proved healing has been effected. A total of 167 patients were assigned on a random basis to either of two treatment groups, control (aluminum hydroxide and magnesium oxide) or sucralfate, and given these medications for 6 months. At the end of this period all medication was withdrawn from both groups and the patients were followed for an additional 12 months. Each patient was examined endoscopically at bimonthly intervals throughout the 18-month observation period. Treatment (sucralfate), ulcer history, size of previous ulcer(s), prestudy healing time, and prestudy healing stage attained (red or white scarring) were associated with a significant reduction in recurrence rate. Ulcer recurrence was markedly reduced in sucralfate patients as compared to control when the pretreatment ulcers had healed slowly (P less than 0.001).

Adult↗
PubMed1980

Posthemiplegic shoulder-hand syndrome, with special reference to related cerebral localization.

Seven autopsy cases of shoulder-hand syndrome following hemiplegia were studied with regard to cerebral localization. One of them showed an isolated brain lesion in the premotor area due to a metastasis from malignant melanoma. Four other cases with cerebral infarction and one with glioblastoma multiforme showed massive brain lesions involving the frontal and parietal lobe cortex in the area supplied by the middle cerebral artery. The seventh case showed a hemorrhagic cerebral lesion in the lentiform nucleus. The most common overlap area in 6 of the 7 cases was located in the premotor region including the anterior part of the motor region. The shoulder-hand syndrome following hemiplegia always develops on the side contralateral to the brain lesion which might cause a unilateral longstanding autonomic dysfunction. As corroborated in a review of the relevant literature, a lesion in the premotor area appears chiefly responsible for the primary mechanism of the shoulder-hand syndrome in post-stroke hemiplegia.

Aged↗
PubMed1980

Murine autoimmune interstitial nephritis and associated antigen: purification of a soluble tubular basement membrane antigen from mice kidneys.

A soluble tubular basement membrane (TBM) antigen has been purified from murine kidneys. The isolated fibers were trypsinized and subjected to zone electrophoresis, Sephadex G-200 gel filtration, and DEAE-cellulose chromatography eluted with linear NaCl concentration gradient while monitoring the TBM antigen activity with specific antihuman TBM antigen. The molecular weight of TBM antigen was estimated to be 30,000 daltons by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified TBM antigen gave a single precipitin line against the specific antiserum and cross-reacted with the TBM antigen similarly purified from other animals such as goat, guinea pig and also with human TBM antigen in an immunodiffusion plate. BALB/c, C3H/He and C57BL/6 mice were immunized twice at 2 weeks' interval with the TBM antigen and adjuvant. Histologically typical interstitial nephritis occurred in BALB/c mice, whereas no nephritis developed in other strains of mice. The inflammatory changes were characterized by intense mononuclear cell infiltration, tubular destruction, and interstitial and periglomerular fibrosis. The serum-stained basement membrane of normal tubules and Bowman's capsules, and no antiglomerular basement membrane activity was detectable in the serum by immunofluorescence. This system provides a simple and useful model in interstitial nephritis in mice produced with a purified TBM antigen.

Animals↗