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Biomedical subjects

S Tsuchida

Publications and source records attributed to S Tsuchida.

At least 289 records · Page 16Linked to original sources
PubMed1984

Role of the pudendal nerves on the dynamics of micturition in the dog evaluated by pressure flow EMG and pressure flow plot studies.

The role of the pudendal nerves on the dynamics of micturition was studied using 16 decerebrated dogs. The voiding cycles were analyzed by pressure flow EMG and pressure flow plot studies under 3 conditions: control, after unilateral, and after bilateral pudendal nerve transection. In the control condition, highly reproducible reflex micturition with bladder contraction and spasmodic rhythmic sphincter contractions was demonstrated. Two patterns were noted following pudendal nerve transection: reflex micturition and overflow incontinence. Even though reflex micturition could be achieved in 9 out of 16 dogs after bilateral transection, there was decreased bladder emptying as well as absence of spasmodic rhythmic sphincter contractions. Overflow incontinence developed in the remaining 7 dogs (5 dogs after bilateral transection and 2 dogs after unilateral transection). It appears that the pudendal nerves play an important role in emptying the bladder of the dog.

Animals↗
PubMed1984

Pelvic nerve innervation of the external sphincter of urethra as suggested by urodynamic and horse-radish peroxidase studies.

In view of the fact that the detrusor vesicae and external urethral sphincter perform closely synergic functions in micturition, experiments were conducted to explore the action of the pelvic efferent neurons on the external urethral sphincter. The pelvic efferent neurons are generally recognized, by urodynamic assessments and histochemical study with the technique of retrograde axonal transport of horse-radish peroxidase, to innervate the vesical detrusor. In 7 of 15 adult dogs studied, the external urethral sphincter continued to show a normal synergic electromyogram pattern with enhanced electrical activity on vesical distention and disappearance of discharges on vesical contraction even after bilateral transection of the pudendal nerves. The electrical discharges ceased in the sphincter only after subsequent bilateral pelvic neurotomy. Horse-radish peroxidase-positive cells were demonstrated in the intermediolateral and intermediomedial nuclei and in the Onuf nucleus of the sacral cord in approximately half the dogs whose pelvic nerve was injected with the plant peroxidase. The results suggest that the pelvic nerve may contain somatic fibers innervating the external urethral sphincter.

Animals↗
PubMed1984

Stereographic urethral pressure profile.

We developed a new system to record urethral pressure curves separately for the anterior, posterior, right and left walls of the urethra. It consists of a catheter with four channels running radially along the axis in 90 degree sectors from the bladder to the external urethral orifice and expresses the data stereographically with a computer system. We called the graph obtained by this recording system 'stereographic urethral pressure profile' (stereo UPP). The stereo UPP consists of a four-directional UPP, a urethral pressure tomogram and a urethral pressure relief diagram. It does not require any more time and manpower than the conventional UPP described by others and causes no pain to the patients. Moreover, this excellent three-dimensional system will enable even the nonspecialist to grasp the functional and morphological status of the urethra at one glance.

Adult↗
PubMed1984

[Role of the striated urethral sphincter in the voiding cycle of the decerebrated dog].

The role of the striated urethral sphincter on the dynamics of micturition was studied using 4 decerebrated dogs. The voiding cycles were analyzed by pressure flow EMG and pressure flow plot studies before and after the administration of suxamethonium. In the control condition, highly reproducible reflex micturition with bladder contraction and spasmodic rhythmic sphincter contractions was demonstrated. After the administration of suxamethonium reflex micturition occurred, but there was decreased bladder emptying as well as absence of spasmodic rhythmic sphincter contractions. The striated urethral sphincter would seen to play an important role in bladder emptying of decerebrated dogs.

Animals↗
PubMed1984

Urethral pressure tomography.

A new method of examination whereby urethral pressure profiles are recorded from the anterior, right, posterior and left urethral walls and tomographically expressed has been devised. The system facilitates three-dimensional delineation of the functional morphology of the urethra, requires no more time and work than does the conventional urethral pressure profile study, or causes no pain in the patient. Stereographic depiction of the continence mechanism of the bladder neck and the function of the external urethral sphincter was achieved by the method, and the degree of urethral oppression was assessed by application of this urethral pressure tomography in patients with prostatic hypertrophy.

Aged↗
PubMed1984

Urometric evaluation of intramural ureter function with continual ureteral perfusion.

Urometry provides a valuable test of ureteral function wherein intraureteral pressure changes associated with ureteral peristaltic contraction are recorded via a fine catheter inserted through the bladder into the ureter. An attempt was made to evaluate the function of the intramural ureter by analysis of urometric wave pattern of resting pressure recorded during the phase of no ureteral peristaltic contraction with continual instillation of sterile water via the catheter at a constant rate. By this technique, the length of functional intramural ureter (FUimL) was estimated to be 10-15 mm and the resting pressure in the intramural ureter (Pr) was between 5 and 10 mmHg in patients with possibly normal intramural ureter (4 ureters). In cases of chronic dysuria with cystographic evidence of morphologic changes in the bladder and with no vesicoureteral reflux (VUR), the FUimL was 5 mm and the Pr 2-3 mmHg. The corresponding values were 5 mm and 1 mmHg with a ureter showing VUR. The data obtained indicated that the product of these parameters, FUimL X Pr, may reflect the function of intramural ureter. The method reported herein being procedurally simple requiring no particular apparatus provides a remarkably valuable test for the evaluation of function of intramural ureter which has been virtually impracticable.

Adolescent↗
PubMed1984

Response of the renal pacemaker to beta 1-adrenoceptor agonist: difference from the cardiac pacemaker.

Electromyographic responses of the canine-pyeloureter to dobutamine, a beta 1-adrenoceptor agonist were investigated in vitro. Dobutamine produced a marked enhancement of potential in the pelvicalyceal (PC-) border, but failed to evoke noticeable responses in the renal pelvis and ureter. Dobutamine, however, made the pacemaker rhythm a little slow. This finding shows that the renal pacemaker is somewhat different form the cardiac pacemaker, pace of which is quickened by dobutamine.

Action Potentials↗
PubMed1984

[Effects of krestin and p-aminobenzoic-N-xyloside sodium salt on activities of drug-metabolizing enzymes and glutathione-related enzymes in rat liver].

Effects of krestin (PSK) and p-aminobenzoic-N-xyloside sodium salt (K-247) (both products of Kreha Chemical Co., Japan) on activities of drug-metabolizing enzymes and glutathione (GSH)-related enzymes were investigated in rat liver. When PSK was administered at a dose of 100 mg/kg body weight, ip every day for 7 or 14 days, the action of UDP-glucuronyltransferase (UDP-GT) on o-aminophenol (o-GT) and those of GSH S-transferase (GST) on both 1, 2-dichloro-4-nitrobenzene ( DCNB ) and 1-chloro-2, 4-dinitrobenzene (CDNB) slightly increased together with increased activities of GSH-peroxidase on both H2O2 and cumene hydroperoxide, were as GSH levels were decreased. When PSK or K-247 was administered at 1% in diet for 4 or 8 weeks, o-GT activity and GST activities with both substrates increased on K-247 feeding, while GST activity for CDNB decreased on PSK and K-247 feedings. These changes were statistically significant but very small. The content of P-450 and the activity of gamma-glutamyl transpeptidase changed little in any administration schedules mentioned above.

4-Aminobenzoic Acid↗
PubMed1983

Purification of detergent-solubilized form and membrane-binding domain of rat gamma-glutamyltransferase by immuno-affinity and hydrophobic chromatography.

A new method to purify papain- or detergent-solubilized form (papain or detergent form) of gamma-glutamyltransferase from rat hepatomas as well as from rat kidney by immuno-affinity column chromatography is presented. The antibody-column was prepared by coupling the anti-kidney papain form antibody, which had been purified by using a kidney papain form-Sepharose column, to CNBr-activated Sepharose 4B. The enzyme bound to the antibody-column was eluted with 0.04 M NH4OH. By this method, detergent forms were purified 300 and 1600-fold in approx. 50% yields from rat kidney and rat ascites hepatoma AH 13, respectively, and the papain form was also purified 16 000-fold in a similar yield from primary hepatoma which has a very low activity of this enzyme. Preparations thus obtained apparently did not contain any peptide other than heavy and light subunit peptides of this enzyme on SDS-polyacrylamide gel electrophoresis. The detergent form of kidney enzyme was preferentially absorbed to a hydrophobic column of aminooctyl-Sepharose, while the papain form was not, suggesting that the detergent form might be adsorbed to the column through hydrophobic interaction of the membrane-binding domain. The domain peptide was also purified by the hydrophobic column after release from the detergent form by papain treatment. The molecular weight of the peptide was estimated to be about 16 000 on SDS-polyacrylamide gel electrophoresis. On double immunodiffusion, the domain peptide reacted with anti-detergent form antibody but not with anti-papain form antibody. The domain-specific antibody was also purified from the anti-detergent form antibody.

Animals↗
PubMed1983

Urodynamic studies on hemiplegic patients after cerebrovascular accident.

Thirty-nine hemiplegic patients were evaluated by complete urodynamic study after cerebrovascular accidents. Computerized tomography was performed to identify localization of the brain lesion. The common urinary symptoms were frequency and urgency incontinence (26/39), while 13 patients complained of dysuria or urinary retention. Ten of the 11 patients who had frontal and internal capsular lesions showed hyperactive bladder, and 6 showed uninhibited sphincter relaxation. Nine of the 10 patients who had putaminal lesions showed hyperactive bladder. Normal sphincter activity was demonstrated in 7 of these 10 patients. In the remaining patients with the other types of lesions, no correlation was found between urodynamic dysfunction and type of brain injury.

Aged↗
PubMed1983

Roles of adrenergic drugs in the renal pelvic pacemaker control of ureteral peristalsis.

Experiments were performed to explore the influence of noradrenaline and isoproterenol on the ureteral peristalsis in the canine pelviureteral system in vitro. The rhythmic changes of intrapelvic pressure no longer became manifest, and simultaneously recorded electromyograms revealed disappearance of peristalsis at sites distal to the upper region of the renal pelvis following application of isoproterenol, while the pacemaker at the pelvicalyceal border continued to discharge at an unaltered rate. It is concluded from our findings that both noradrenaline and isoproterenol act upon conduction of peristalsis rather than on the pacemaker as such, thereby facilitating or inhibiting the conduction, respectively, in their behavior toward ureteral peristalsis. The results also have demonstrated that the renal pelvic pressure recorded during treatment with isoproterenol did not reflect pacemaker activity of the renal pelvis.

Action Potentials↗
PubMed1983

Urethral response to nerve stimulation measured by strain gauge force transducer.

This paper presents a study of urethral responses to efferent nerve stimulation in dogs. The pelvic, hypogastric and pudendal nerves were stimulated using a programmed instrument under three different in vivo conditions. The preparations consisted of: (A) bladder, urethra, and rectum; (B) urethra and rectum, and (C) urethra only. Stimulation of the pelvic nerve in preparation A resulted in elongation and constriction of the mid and proximal urethra with a resultant rise in pressure. However, this response was reduced in preparations B and C. Similar observations were made with hypogastric nerve stimulation. The results of pudendal nerve stimulation were significantly different, in that the pressure rise occurred in the mild and distal urethra and varied in elongation response.

Animals↗
PubMed1983

Discrepancy between the renal pelvic fluctuations and the pacemaker discharges at the pelvi-calyceal border.

Experiments were performed in vitro to explore the influence of isoproterenol on the canine pelvi-ureteral system. The rhythmic changes of intrapelvic pressure became no longer manifest and simultaneously recorded electromyograms revealed disappearance of peristalsis at sites distal to the upper region of renal pelvis following application of isoproterenol, while the pacemaker at the pelvi-calyceal border continued to discharge with an enhanced pace. This result has demonstrated that the renal pelvic pressure recorded during treatment with isoproterenol did not reflect the pacemaker activity of the renal pelvis.

Animals↗