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Biomedical subjects

S Sakuma

Publications and source records attributed to S Sakuma.

At least 379 records · Page 21Linked to original sources
PubMed1980

[Methods for intravenous injection and blood collection in the guinea pig via veins of the forelimb without anesthesia (author's transl)].

Methods for intravenous injection and blood collection by way of the forelimb in guinea pigs without anesthesia were investigated, with the results as follows: 1) Both the accessory cephalic and cephalic veins were found available for injection as well as for drawing blood samples. Venipuncture was accomplished with greater ease by using the accessory cephalic vein. 2) The following procedure has proven practical. (i) Clip the hair over the site. (ii) Restrain the animal to immobilize the foreleg in slight extension by grasping it at the elbow joint with the thumb and forefinger over its dorsal and ventral aspects, respectively. (iii) After disinfecting the skin surface with alcohol, perform injection using an appropriate syringe with a 27 (1/4) or 25 (1/3) gauge intravenous needle, or puncture the vein with a 23 (1/2) gauge i. v. needle to collect the blood that drips out.

Animals↗
PubMed1980

Influence of 6 alpha-methylprednisolone 21-sodium succinate on the pituitary, adrenal glands, and the gastrointestinal tract in rats. (Comparative toxicity study with hydrocortisone 21-sodium succinate).

A comparative toxicity study with 6 alpha-methylprednisolone 21-sodium succinate (MPS) and hydrocortisone 21-sodium succinate (HCS) was carried out on male rats of the Wistar strain. MPS and HCS were administered intravenously to rats in dose levels of 20 or 100 mg/kg/day for 14 days. There were no significant differences in the pituitary weights in MPS and HCS groups, but weight of the adrenal glands decreased with increasing dose levels of MPS and HCS. Histopathological examination of MPS and HCS treated rats revealed atrophy of the zona fasiculata in the adrenal cortex which correlated with dose levels. The decline of serum corticosterone levels in rats correlated with increasing dose levels of MPS and HCS. It can be concluded that the inhibitory effect of MPS on the pituitary-adrenal system in rats was the same as HCS. No ulceration was observed in the gastro-intestinal tract of MPS of HCS treated rats.

Adrenal Glands↗
PubMed1979

Estrogen and progesterone receptors in human breast cancer with concomitant assay of plasma 17beta-estradiol, progesterone, and prolactin levels.

The specific estrogen receptor in the cytosol of human breast cancer tissue was assayed in 217 primary cases. The specific progesterone receptor was also assayed in 48 cases as evidence of estrogen action on the tissue. Both receptors were positive in 45.8% of all cases. Plasma 17beta-estradiol and progesterone were assayed concomitantly with these steroid receptors. The higher hormone levels were found in the cases with fewer receptor binding sites. The relationship between 17beta-estradiol levels in tumor cytosol and the number of binding sites was more clearly observed. Plasma prolactin levels, however, showed no correlation with the number of receptor binding sites or the plasma levels of sex steroids. None of these assayed substances had a clear correlation with the histological type of tumor or the clinical stage of the disease.

Binding Sites↗