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Biomedical subjects

S Matsuno

Publications and source records attributed to S Matsuno.

At least 343 records · Page 19Linked to original sources
PubMed1989

A kinetic study on secretion and elimination of endogenous thyrotropin in the thyrotropin-releasing hormone test.

Serum thyrotropin (TSH) concentrations in normal young men were measured by a high-sensitivity immunoradiometric assay before and after intravenous administration of 500 micrograms of TSH-releasing hormone (TRH). A kinetic model was applied to evaluate the secretion rate both before (V0) and after (V0 + V* at maximum rate) the administration of TRH, the elimination constant (K), the latent time (L) between TRH administration and start of the stimulated secretion, and the total amount of TSH (T) released in response to TRH. V0, V* and T varied widely from individual to individual, but correlated well with TSH before TRH administration (r = 0.93, 0.80 and 0.87, respectively). A few minutes (1.89 +/- 1.30 min) after the administration of TRH, the secretion of TSH (0.025 +/- 0.016 microU/min ml) was stimulated, and the total release over about 1 h was 12.5 +/- 5.6 microU/ml. Serum TSH was maximum at 31.5 +/- 5.7 min. The half-time of disappearance of TSH was 42 +/- 9 min. These data confirm that the stimulated secretion continues for more than 30 min, and that the pituitary releases 43.2 +/- 22.9 mU of TSH (assuming the distribution volume of TSH is 5.8% of body weight) in response to TRH, an amount which correlates closely (r = 0.91) with TSH before TRH administration.

Adult↗
PubMed1989

Effect of blood exchange transfusion as an initial treatment of acute hemorrhagic pancreatitis.

The effect of blood exchange transfusion on experimental acute pancreatitis was investigated. Acute hemorrhagic pancreatitis was induced in rats by the injection of sodium taurocholate into the pancreatic duct. One hour after the induction of pancreatitis, blood exchange transfusion was carried out in these rats. The survival rates by 24 hr significantly increased in the exchange transfusion group as compared to those in the non-treated control group. Blood exchange transfusion, though had not significant effect on the macroscopic findings at autopsy, reduced pulmonary edema. These results suggested that blood purification by exchange transfusion may be beneficial for patients with acute pancreatitis, especially at an early stage.

Amylases↗
PubMed1989

Plasma amino acid abnormalities in liver disease: comparative analysis of idiopathic portal hypertension, extrahepatic portal occlusion and liver cirrhosis.

To clarify the clinical significance of specific plasma amino acid abnormalities occurring in liver disorders with portal-systemic shunting, plasma amino acids and insulin levels were measured in idiopathic portal hypertension (IPH), extrahepatic portal occulusion (EHPO), and liver cirrhosis (LC). Three branched chain amino acids (BCAA: valine + leucine + isoleucine) were decreased in all three diseases in comparison with controls. Since plasma insulin measured during oral glucose tolerance tests did not specifically rise in LC, reduction of BCAA is not merely ascribed to hyperinsulinemia. Either portal-systemic shunting or some extent of liver damage may contribute to a fall in BCAA. Two aromatic amino acids (AAA: phenylalanine + tyrosine), which were within the normal range in EHPO and IPH, showed a marked increase in LC. Thus, changes of AAA probably mainly reflect the severity of the liver disease. The molar ratio of BCAA/AAA (MR) significantly correlated with ICG k, ICG R15, PT and the sum of blood ammonia in an oral ammonia tolerance test which may reflect the degree of hepatic disorder. MR diminished in the following decreasing order: controls, EHPO, IPH and LC.

Amino Acids↗
PubMed1989

Taurocholate-induced gastric damage in rats with obstructive jaundice.

We examined the severity of taurocholate-induced gastric mucosal damage in rats with obstructive jaundice. Oral administration of 100 mM taurocholate induced severer gastric mucosal damage in rats with jaundice than in intact rats. Pretreatment with 5 mM taurocholate significantly protected the gastric mucosa against 100 mM taurocholate-induced damage, suggesting adaptive cytoprotection in rats with jaundice. The reductions in the gastric mucosal potential difference and hexosamine contents in the gastric mucosa in response to 100 mM taurocholate were significantly attenuated by prior administration of 5 mM taurocholate in both intact rats and rats with jaundice.

Animals↗
PubMed1989

[Inhibitory effect of anti-platelet prostaglandin on liver metastasis of hamster pancreatic cancer].

Coagulation system and platelets play an important role in the stage of lodgement of tumor cells. We examined abilities of human and hamster pancreatic cancer cell lines to aggregate platelets in vitro, and investigated the effect of prostaglandin E1, I2, on artificial liver metastases of pancreatic cancer in Syrian golden hamster. Platelet aggregating activities were found in five out of six human pancreatic cancer cell line and thromboplastin likes activity in five cell lines. Diisopropanolnitrosamine induced hamster pancreatic cancer cells (HPK-1) were able to aggregate platelets both in vitro and in vivo and these activities were inhibited by prostaglandin I2. Hamster was inoculated intraportally with 1 X 10(6) HPK-1 cells. After two weeks autopsy of these hamsters revealed multiple metastatic nodules on liver surface. In this model we administered prostaglandin E1, I2 into the portal vein five minutes before cell inoculation. Number of liver surface nodules were significantly decreased to 33.1 + 7.0, 11.0 + 9.6 in hamster given 100g PGE1 PGI2 before cell inoculation, compared with control group of hamsters (62.0 + 6.6 PH9.3, 66.1 + 13.9 PH7.4). But administration of prostaglandin after cell injection was not effective. In all cases none of extrahepatic metastases were noted. Inhibitory action of PGE1 PGI2 on liver metastasis is suspected to be related to inhibition of platelet aggregation.

Alprostadil↗
PubMed1989

[Changes in hepatic and renal tissue blood flows in cirrhotic rat in hemorrhagic shock].

The purpose of present study is to elucidate the influence of hypotension on hepatic and renal tissue circulations in cirrhosis. The changes of hepatic and renal tissue blood flows (TBF) were studied in hemorrhagic shock using cirrhotic rats. The hepatic and renal energy charges (EC) were measured in normal and cirrhotic rats. Hepatic TBFs of the cirrhotic rats decreased to 74% of the normal, portal pressure increased to 140%, but renal TBF was not changed significantly. In hemorrhagic shock induced by blood exsanguination, hepatic and renal TBFs of normal rats were restored to the preshock levels by transfusion of shed blood, but those of cirrhotic rats were not restored. Furthermore, the hepatic and renal ECs of normal-shocked rats were not different from those of control rats, but the ECs of cirrhotic-shocked rats were decreased significantly. These findings suggest that hypotension under cirrhosis disturbs hepatic and renal tissue circulations and energy metabolisms also in a period of after-shock phase.

Animals↗
PubMed1989

[Effect of ileo-jejunal transposition (IJT) on gastrointestinal hormones and intestinal structure in dogs].

The effects of ileo-jejunal transposition (IJT) on gastro-intestinal hormones and intestinal structure have been studied in 9 mongrel dogs. IJT was performed by isoperistaltic interposition of the distal fourth of the small bowel in the jejunum 15 cm distal from the ligament of Treitz. A test meal (carbohydrate- and fat-rich) loading was carried out in 5 dogs before and 4 and 12 weeks after the operation. Plasma concentrations of gastrointestinal hormones (GLI, GI, GIP and gastrin) were measured by radioimmunoassay using the antibodies. The six mongrel dogs were used for the histological studies. Following IJT hyperenteroglucagonemia was observed, especially in postprandial state. An increase of the mucosal thickness in the whole intestine was observed after IJT. This suggested the possibility that enteroglucagon stimulates intestinal mucosal growth as a circulating hormone. Postprandial plasma GIP levels after IJT were significantly lower at the 90, 120 and 150 min after the test meal loading than those of the preoperative state. Plasma gastrin levels were no significant differences before and after surgery. These observations lead us to conclude that enteroglucagon may play an important role in intestinal adaptation mechanisms after IJT.

Animals↗
PubMed1989

[Scintigraphic estimation of dearterialization after transcatheter hepatic arterial embolization therapy].

Radionuclide angiography was performed by bolus injection of 99mTc-phytate in 10 patients with liver neoplasms before and after the transcatheter hepatic arterial embolization therapy. The ratio of the slopes of the upstrokes for the hepatic arterial and abdominal aortic first transit curves constitutes the regional hepatic arterial to aortic blood flow ratio (RHA/A ratio), which represents quantitatively the arterial blood flow of hepatic region of interest as a fraction of aortic blood flow. The degree of embolic dearterialization was estimated by the change of this ratio derives from the same region of interest for each patient before and after the embolization. The values and post-embolic reduction of the ratio correlated well individually with the angiographic features and other patients' data. The arterial blood supply to the tumor could not be reducted using Lipiodol alone as the embolic material in only 1 case. The finding that the splenic arterial to aortic blood flow ratio (S/A ratio), calculated in the same way, was practically unchanged after the hepatic arterial embolization indicates the good reproducibility of these measurements. This noninvasive method may be helpful to evaluate the therapeutic effect of hepatic arterial embolization.

Aged↗
PubMed1988

Pancreatic function and rehabilitation after pancreaticoduodenectomy.

Postoperative pancreatic function and rehabilitation were monitored in 149 patients who had had reconstruction of the digestive tract by Child's method, out of a total 151 patients who had undergone pancreaticoduodenectomy. The occurrence of peptic ulcer following Child's method may be prevented by the resection of a wider area of the stomach. Because the source of gut hormone secretion is removed by the pancreaticoduodenectomy, the secretion of this hormone is subsequently reduced and thus metabolic abnormalities also occur. If careful attention however, is paid to the maintenance of residual pancreatic function most patients who undergo pancreaticoduodenectomy can be expected to lead reasonably normal lives.

Duodenum↗
PubMed1988

Characterization of a human rotavirus strain which is possibly a naturally-occurring reassortant virus.

We investigated the antigenic and genetic characters of one of two human rotavirus strains 69M and 57M isolated in Indonesia, both of which showed a "super-short" RNA electrophoretic pattern (A. Hasegawa et al., Microbiol. Immunol. 28, 719-722, 1984). By an enzyme-linked immunosorbent assay with subgroup-specific monoclonal antibodies, one virus, strain 57M, was found to have subgroup II antigenicity. The cross-reaction of this strain, in a plaque neutralization test, with a serotype 4 strain was high whereas that of strain 69M was low. When radiolabeled RNA probes prepared from this virus were hybridized with RNAs from reference strains of different serotypes, treated with S-1 nuclease and then subjected to polyacrylamide gel electrophoresis, we found that (i) RNA segment 10 (the super-short segment) hybridized with that of another super-short pattern virus, strain 69M; (ii) segment 7, coding for a neutralization antigen, hybridized with that of the serotype 4 virus; (iii) segment 6, coding for a major inner-shell protein, hybridized with that of a serotype 1 virus; and (iv), some other segments hybridized with those of the reference viruses of serotypes other than 2 and 3. We suspect that this strain is possibly a naturally-occurring reassortant virus whose genetic segments are derived from different human rotaviruses.

Antibodies, Monoclonal↗
PubMed1988

Peptic ulcer and gastric acid secretion in patients with insulinoma.

We evaluated the incidence of associated peptic ulcers and the gastric acid secretion in our 20 series of insulinoma, and obtained the following results: 1) Four out of 20 patients (20%) with insulinoma were associated with peptic ulcers. 2) Except for the maximum acid concentration, the preoperative gastric secretion tests, such as the basal acid output and maximum acid output, failed to indicate hyperacidity in those patients. And patients with a short duration of illness by insulinoma neither showed hyperacidity nor were associated with peptic ulcers. 3) A tendency of reduced gastric acid secretion and the amelioration of peptic ulcers was observed in those who underwent the extirpation of insulinoma. 4) In the most recent case received gastric analysis with continuous monitoring of blood glucose level, an increase of basal acid output was observed during the hypoglycemic period in which the blood glucose levels reduced to around 30 mg/100 ml. These results indicate that the low blood glucose level and the high gastric acid secretion produced under the condition on an empty stomach, may be important factors related to the high association rates of the peptic ulcers in patients with insulinoma.

Adenoma, Islet Cell↗