[The effect of indolacin in rat periapical lesion and radioimmunoassay determination of prostaglandin E2].
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Animals↗
Biomedical subjects
S Li
Publications and source records attributed to S Li.
[Construction of genomic library of L. interrogans serovar lai and preliminary study of recombinant plasmid pDC38].
A genomic library, consisting of approximate 12 000 recombinants, has been constructed for the Leptospira interrogans serovar lai in E. coli. using pUC18 as the vector. Hybridization analysis with the DNA fragment containing OmpL1 gene was performed and 10 positive clones were screened from the genomic library. One of the positive clones, designated pDC38, showed hybridization signal with the DNA of 7 serovars 8 strains of pathogenic leptospires, but not with the DNA of nonpathogenic leptospires (L. biflexa), Leptonama illini, E. coli., and 2 strains of L. interrongans.
DNA, Recombinant↗
[Diagnosis of meconium aspiration syndrome by fluorometry technique].
OBJECTIVE: To establish a new diagnostic method of neonatal meconium aspiration syndrome (MAS). METHODS: The fluorometry technique was used for examination of urine samples from three groups of neonates. The neonates were divided into 3 groups: Group I, MAS (n = 17); Group II, no MAS, but amniotic fluid with meconium stain (n = 24); Group III, normal control (n = 27). Urine fluorescence meconium index (UFMI) and urine meconium index (UMI) were calculated. RESULTS: UFMI values of group I were higher than that of group II and III. The sensitivity and specificity of UFMI were 100% and 97% respectivety. However, it were 51% and 48% respectivety in UMI. CONCLUSIONS: UFMI is an index for identification of Zn-coproporphycin, and it is a more sensitive index for diagnosis of MAS.
Coproporphyrins↗
[Peribulbar anesthesia and intraocular lens implantation].
We studied the effectiveness of peribulbar anesthesia in 146 patients undergoing cataract surgery and intraocular lens implantation. The results revealed that the analgesic and akinetic effects obtained from peribulbar anesthesia are the same as or even better than that obtained from retrobulbar anesthesia. In the mean time, the use of peribulbar injection can avoid or reduce the serious complications associated with the retrobulbar injection. In this report, the method of peribulbar anesthesia was introduced and its mechanisms and effectiveness were discussed. The pre- and post-anesthetic intraocular pressures were especially observed and compared. It is safe to perform the operation 10 minutes after the administration of the peribulbar anesthesia.
Adolescent↗
[Posterior chamber intraocular lens implantation in diabetics].
93 eyes having undergone extracapsular cataract extraction with posterior chamber intraocular lens implantation were evaluated prospectively. There were two groups of patients, including 38 diabetics with or without nonproliferative retinopathy and 38 nondiabetics. The comparison between the two groups demonstrated that there was no significant difference in intra-operative or postoperative complications. The post-operative corrected visual acuities of 0.5 or better were achieved in 26 of 47 (55.3%) of diabetic eyes and in 30 of 46 (65.2%) of nondiabetic eyes (P > 0.05). Based on this study, it is considered that nonproliferative diabetic retinopathy is not a contraindication of intraocular lens implantation. However, post-operatively, diabetic patients should be followed more closely and if any post-operative complication does occur, it should be treated promptly.
Aged↗
[The differences in corneal shape between myopic and normal eyes].
The corneal shapes of 35 eyes with moderate and mild degrees of myopia compared with those of 35 normal eyes were studied by a computerized video keratoscope (TMS-1). The results show that the surface regularity index (SRI), the refractive power at central optical zone, the simulated keratoscopic reading (Simk), the average corneal power of zones 3mm, 5mm, 7mm in diameter centered at the corneal center of the eyes with moderate and mild degrees of myopia are greater than those of the normal eyes (P < 0.05). However, the surface asymmetry index (SAI), the differences of corneal powers between the first and the 15th ring and between the first and the 25th ring in both groups are of no statistical significant differences (P > 0.05). It is suggested that the corneal refractive power of myopic eye be much higher than that of the normal eye. The authors propose the first time that the pathogenesis of some eyes with moderate and mild myopia may be the increase of corneal refractive power which results from traction of sclera near the limbus due to accommodation and discuss it in detail.
Adolescent↗
[Afferent projections to the central superior raphe nucleus of brain stem and its relation to acupuncture anesthesia].
The afferent projections to the central superior raphe nuclus (CS) in the rat were investigated by means of HRP retrograte tracing method. 3% WGA-HRP 0.05-0.1 microliters was injected into the CS in each of 11 rats. The labeled cells were found in the medial part of lateral habenular nucleus, posterior hypothalamic area, lateral subnucleus of interpeduncular nucleus, dorsal raphe nucleus, periaqueductal gray matter, dorsal paramedial nucleus, preposital hypoglossal nucleus, dorsal paragiganto-cellular nucleus, magnum raphe nucleus, and fastigatum of cerebellum. The results provide morphological basis for researching function of the CS in acupuncture anesthesia.
Acupuncture Analgesia↗
A study on HSV-1 corneal potential infection by in situ nucleic acid hybridization.
PURPOSE: To evaluate the possibility of HSV-1 corneal latency by in situ nucleic acid hybridization in animal models. METHODS: 20 normal New Zealand White (NEW) rabbits were used, 14 of them were inoculated bilaterally with 3 x 10 PFU/ml of McKrae strain HSV-1 by intrastromal injection. 22/28 eyes developed typical herpes simplex keratitis (HSK) diseases. At 60 day postoperation (PI), 4 latent corneas were transplanted to one eye of 4 noninfected NZW rabbits and removed 2 weeks PI. Corneas at all time intervals of infection and two weeks after PKP were detected for presence of HSV-1 antigen and nucleic acid sequences by using clonal IgG HSV-1 antibody and biotinylated HSV-1 DNA probe individually. RESULTS: The results showed that the HSV-1 DNA sequences were retained within the corneal epithelium and anterior stromal keratocytes during acute diseases, while the corneas during latent infection and postoperation, the HSV-1 DNA sequences were retained only within the stromal layer with negative HSV-1 antigen staining. CONCLUSIONS: These results strongly suggest that the cornea may be capable of harboring latent HSV-1.
Animals↗
Nd: YAG laser lysis of the fibrinous membrane and remnant substance on the anterior surface of intraocular lens.
PURPOSE: To determine the effects of Nd: YAG laser to disrupt the fibrinous membrane and remnant substance on the anterior surface of intraocular lens. METHODS: Nd: YAG laser was applied on 23 cases of fibrinous membrane formation and 8 cases of remnant substance on the anterior surface of intraocular lenses (IOL) which had not responded well to steroid therapy. Eighteen cases were male and 13 female. The mean age was 49.7 years (range, 5 approximately 78 years). The interval between IOL implantation and laser therapy was 0.5 approximately 30 months in the fibrinous membrane cases and 3 approximately 10 days in the remnant substance cases. The energy applied was 0.8 approximately 3.0 mJ/exp. with 2 approximately 112 exposures. Mean follow-up period was 3.6 months. RESULTS: Complications during therapy included only 2 cases of slight iris bleeding. Visual acuity after therapy was improved 1 line in 16 cases, 2 lines in 11 cases, 3 lines in 1 case, 4 lines in 1 case and 5 lines in 2 cases. No post-therapy complication was found. CONCLUSIONS: Nd: YAG laser lysis is an effective alternative to remove the fibrinous membrane and remnant substances on the anterior surface of IOL.
Adolescent↗
Modified scleral flap incision to reduce corneal astigmatism after intraocular lens implantation.
PURPOSE: To investigate a simple method during extracapsular cataract extraction with posterior chamber intraocular lens implantation in order to reduce surgically induced corneal astigmatism. METHODS: A modified scleral flap incision was used in the extracapsular cataract extraction with intraocular lens implantation and the postoperative changes in corneal astigmatism was observed. RESULTS: The peak value of postoperative corneal astigmatism was 3.60 D, and the corneal astigmatism regression was 2.11 D, surgically induced astigmatism was less significant in modified scleral flap incision group than that in conventional limbal incision group (P < 0.05). CONCLUSIONS: The modified scleral flap incision is an ideal incision for cataract extraction with intraocular lens implantation when phacoemulsifier is not available.
Aged↗
Primary posterior chamber intraocular lens implantation in traumatic cataract with posterior capsule breaks.
BACKGROUND: In patients with incomplete posterior capsule support, posterior chamber intraocular lenses (PC-IOLs) were implanted with both haptics transscleral fixation. This causes more damage to the eye and may result in more complications. In patients with small posterior breaks, non-fixation or single haptic fixation may be adequate. METHODS: Thirty-two consecutive patients of traumatic cataract with posterior capsule breaks caused by penetrating eye trauma were retrospected. Posterior chamber intraocular lenses were implanted in all these patients with three techniques, ie, without fixation, with single haptic fixation and with both haptics fixation. The selection of the technique was based on the position and size of the posterior capsule. The follow-up period was 21 days to 28 months (mean, 15.2 months). RESULTS: Intra-operative problems included ciliary body bleeding (two patients, 6.25%) and enlargement of posterior capsule breaks (2 patients, 6.25%). Postoperative visual acuity was 0.5 or better (corrected) in 28 case (87.5%) and 0.1-0.4 in four patients (12.5%). Postoperative complications included hyphema (6 eyes, 18.8%), transient intraocular pressure elevation (6 eyes, 18.8%), transient hypotention (7 eyes, 21.8%). Postoperative IOL position were good except one case of IOL tilt. No pupillary capture or endophthalmitis was found. CONCLUSIONS: Not all PC-IOLs have to be fixed by two haptics. In patients with small posterior capsule breaks, PC-IOL may not be fixed or fixed by only one haptics.
Adolescent↗
The changes of pattern reversal visual evoked potentials in normal infants.
PURPOSE: To study pattern reversal visual evoked potential (PVEPs) and determine the developmental character and mature time of visual function in normal infants at different months of age. METHODS: PVEPs were recorded from 115 normal infants at 3, 6, 9, 12 months age. P1 latency for different checks (1 degree 40', 25', 6') was analyzed and compared to those of normal adults. Changes of N1, N2 latency of PVEPs were also examined. RESULTS: P1 latency for all checks (1 degree 40', 25', 6') was significantly longer at 3 months than at 6 months of age (P < 0.05), but no significant differences can be seen after 6 months of age for larger (1 degree 40') and intermediate (25') checks (P > 0.05). P1 latency for larger checks (1 degree 40') reached adult level after 3 months of age, but not for the intermediate check (25'), while P1 latency for small check (6') presented the character of fluctuation. CONCLUSION: The visual system continued to develop after birth and appeared a certain regularity. Our results showed that P1 latency for larger check (1 degree 40') reached adult levels after 3 months of age. But P1 latency for intermediate check still has not reached adult levels after 3 months of age. To determine the age at which adult levels are finally reached, infants of 12 months and older must be tested. The reason why P1 latency for smaller check (6') presented the character of fluctuation should be the temporal tuning function developing much more slowly.
Child Development↗
[The visual evoked potentials of non-amblyopic eyes in children with amblyopia].
We investigated the characteristics of visual evoked potentials (VEPs) of the eyes with normal visual acuity in 84 children of whom 20 were normal, 28 of anisometropic amblyopia, 8 of anisometropic amblyopia already cured, 8 pairs of monozygotic and 6 pairs of dizygotic twins with amblyopia. The results showed that although the fellow eye of an amblyopic eye or the cured amblyopic eye had normal visual acuity, the VEPs presented abnormal, the marked abnormality being the prolongation of the latency of P100 peak. No significant difference was found in VEPs between the eye with normal visual acuity and the eye with amblyopia in the monozygotic twins with the same genetic background (P > 0.05). This study demonstrated that possibly the clinical examinations of visual acuity and VEPs reflect different visual information processes, and the "normal" eyes of amblyopes are not normal.
Amblyopia↗
[Continuous circular capsulorhexis in extracapsular cataract extraction].
UNLABELLED: To investigate clinically the advantage, method and prevention of complication continuous circular capsulorhexis, a surgery consisting of continuous circular capsulorhexis, extracapsular cataract extraction and one-piece PMMA intraocular lens (IOL) implantation was performed on 52 eyes. The control was "canopener" capsulotomy made in 40 eyes. The residual anterior capsule and loop fixation of IOL were observed by dilatation of the pupil and gonioscopy 1 month after the operation. RESULTS: Postoperatively an anterior capsular opening of 6.5 approximately 7.0 mm in diameter with sharp edge could be seen in 48 eyes and the fixation of both loops in the capsular bag was found in 44 eyes (84.6%) in capsulorhexis group and 21 eyes (52.5%) in "canopener" capsulotomy group. It is demonstrated that continuous circular capsulorhexis could maintain the integrity of the capsular bag and increase the chance of achieving a fixation of IOL in the bag.
Aged↗
[The mRNA expression of CD2, CD4 and CD8 in peripheral blood lymphocytes from the patients with acute lymphoblastic leukemia and non-Hodgkin's lymphoma].
The paper for the first time reports the detection of the levels of CD2, CD4 and CD8 mRNA expression in peripheral blood lymphocytes from 9 cases with T-acute lymphoblastic leukemia (ALL) and 10 cases with T-non-Hodgkin's lymphoma (NHL) by using RNA dot blotting technique. It was found that the levels of CD2 mRNA in the two groups of patients were significantly higher than those in the normals. The ratios of the CD4 to CD8 mRNA were also increased in patients with NHL. The significance of this study was discussed.
Adult↗
[The study of the relationship between the granule size of immunoliposomes and the conjugate ratio of F(ab')2 of McAb HI30 (CD45)].
The immunoliposomes were prepared by conjugating the F(ab')2 of monoclonal antibodies HI30(CD45) labelled by 125I with the liposomes labelled by 131I, and then were separated by a sephacryl S-300 column. The chromatography showed that the liposomes prepared by ultrasonic method were not homogeneous, and the liposomes of smaller granule size had a higher conjugate ratio. It was also found that the fragment F(ab')2 of McAb conjugated to liposomes could enlarge their granule size.
Animals↗
[Surgical treatment of spinal tumors: a report of 30 cases].
With the advance of spinal surgery in the last decade, surgical treatment of spinal tumors has been no longer limited to sole laminectomy. The principles of surgical treatment of spinal tumors include: (1) anterior approach for the anterior lesion and posterior approach for the posterior lesion, (2) combined anterior and posterior approach for extensive lesions and (3) internal fixation for spinal stability. 30 cases of spinal tumors were treated on the basis of the above guiding principles and 83.3% (25 of 30 cases) showed excellent or good outcomes.
Adult↗
[The study of grading standard photos of oblique flashlight test].
PURPOSE: We try to establish a convenient, fast, inexpensive and accurate screening test and to make a grading standard. METHODS: 1. In 120 eyes, we measured axial anterior chamber depth (AACD), peripheral anterior chamber depth (PACD), antrior chamber angle (ACA) and took iris pictures under slitlamp microscope imitating the oblique flashlight test. The clear photos from 107 eyes were measured by computerized image analysis to determin the ratio of the width of nasal iris and nasal iris light band (ILBR). Then the linear correlation of ILBR with AACD, PACD and ACA was analyzed statistically. The ILBR was graded according to the analytic results. RESULTS: 1. Relationship of ILBR with PACD, AACD and ACA: the linear correlation analysis showed a positive correlation relationship among ILBR, PACD and AACD (r = 0.9451, P = 0.0001 and r = 0.8725, P = 0.0001), and showed a negative correlation relationship between ILBR and ACA (r = -0.7582, P = 0.0001). 2. ILBR grading standards: grade 1. ILBR < or = 1/5, the gonioscopy usually demonstrates a dangerously narrowed angle (N3-4); grade 2, ILBR > 1/5- < or = 1/4, the gonioscopy will always show a capable of closure angle (N2-3); grade 3, ILBR > 1/4- < or = 1/3, that angle is incapable of closure (N1-2); grade 4, ILBR > 1/3- < or = 1/2, the most angles show wide (W-N1); grade 5, ILBR > 1/2, the gonioscopy shows all wide angle. The linear correlation analysis also showed correlation relationship among ILBR grades PACD, AACD and ACA. CONCLUSION: Through arational ILBR grades and a set of grading standard photos, it is found that the test is not only convenient, fast, and inexpensive, but also accurate as well. The test not only plays an important role in PACG screening, but also may be applied widely to estimate shallow AACD and narrow angle in the clinic.
Anterior Chamber↗