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Biomedical subjects

S Kikuchi

Publications and source records attributed to S Kikuchi.

At least 487 records · Page 27Linked to original sources

[Compartmental syndrome of the lower limb--basic evaluation].

In order to clarify the structural background for compartmental syndrome in the lower limb, biomechanical study was carried out on cadavers and pathological investigation was done on amputated limbs in clinical cases. Experimental ligation of arteries was made on rats and histochemical analysis of the ischemic muscles was also performed. Furthermore, the pressure inside the leg compartments of normal controls was measured. Based on these observations, the following conclusions were drawn. Some structural specialties of compartments are responsible for the clinical fact that anterior compartment syndrome is most frequently seen in the lower limb. Circular fibers existing at the wall of the anterior compartment is probably related to frequent occurrence of a high pressure in the compartment. In the muscle with ischemic changes, regenerating muscle fibers are found just under the fascia. This fact suggests that the muscle fibers have possibilities of regaining their function. Ischemic change of nerves is more severe in the peroneal than in the tibial nerves. This fact may be due to the anatomical specialties of compartments rather than circulatory characteristics of nerves themselves.

Adult↗

[Determination of urinary immunosuppressive acidic protein in cancer patients].

The urine levels of immunosuppressive acidic protein (IAP) were measured in patients with and without cancer, using passive hemagglutination inhibition test and 24-h urine samples. In 23 control healthy adults, the urine levels of IAP were 0.13-1.3 mg/day. If an elevation of IAP levels were found in 46 (48%) of 96 cancer patients. Elevated urine IAP levels were found in 6 (18%) of 34 patients with non-cancerous gastrointestinal tract diseases. Increased urine IAP was demonstrated in 11 (58%) of 19 non-cancer patients with rheumatoid arthritis.

Adolescent↗

[Brain serotonin metabolism in experimental cerebral ischemia and the effect of coenzyme Q administration].

Bilateral ligation of common carotid arteries of male Wistar rats was produced during fasting for the periods of 15, 30 and 60 min. Thereafter the animals were killed by decapitation, and the brain was divided into the forebrain (ischemic portion), and the brain stem and the cerebellum (blood flow maintained portions by the vertebrobasilar artery system). The above three portions were subjected to fluorophotometry of serotonin (5-HT) and 5-hydroxindole acetic acid (5-HIAA) according to the method described by Curzon. In the different series of the studies, rats pretreated with coenzyme Q (CoQ) were exposed to bilateral ligation of common carotid arteries for 60 min, and the CoQ effect upon the serotonin metabolism was investigated. The results obtained were as follows; 1. The 5-HT levels in the forebrain progressively reduced starting at 15 min (p less than 0.01), and reached to 80.2% of the control value at 60 min (p less than 0.001), while those of the brain stem and the cerebellum showed no significant changes. 2. The time-course changes of 5-HIAA levels up to 60 min were not remarkable in the forebrain and the cerebellum, while those of brain stem revealed a decreasing tendency. 3. CoQ pretreatment to sham-operated rats disclosed a gross increasing trend of 5-HT and 5-HIAA concentrations in the brain stem. 4. CoQ preadministrated brains, of which bilateral common carotid arteries were ligated for 60 min, showed a statistically significant elevation of the 5-HT in the brain stem (p less than 0.01) and in the cerebellum (p less than 0.05), while the 5-HIAA level of CoQ preadministrated brains was higher only in the brain stem as compared to that of shamoperated rats (p less than 0.05). 5. The above results would lead to the hypothesis that CoQ treatment could activate brain serotonin metabolism in case of cerebral infarct.

Animals↗

Age-related changes in the glutamate metabolism of cerebral cortical slices from rats.

The glutamate metabolism in cerebral cortical slices from young (12 weeks) and aged (100 weeks) rats was studied. A highly significant reduction of low affinity glutamate uptake and its metabolism in the aged rats by 14.5% of the amount of the young rats was observed (P less than 0.001). When the ratio of the radioactivity of respective metabolite divided by the sum total radioactivity of overall glutamate metabolites was compared, the incorporation to aspartate was significantly small (P less than 0.01), while that of the CO2 liberated and the GABA synthesized were increased (P less than 0.05). These results support the hypothesis that in cerebral cortical slices from aged rats the transamination of glutamate is suppressed and its decarboxylation is enhanced despite markedly reduced low affinity glutamate uptake into the cerebral cortex. The difference could be explained by the fact that cerebral cortical slices from aged rats are more vulnerable to anoxia than those from young rats when exposed during slice preparation.

Aging↗

New malonyl-CoA-dependent fatty acid elongation system in Mycobacterium smegmatis.

Beside de novo fatty acid synthetase, two kinds of fatty acid elongating systems have been found in Mycobacterium smegmatis; they are the malonyl-CoA-dependent, acyl carrier protein (ACP)-requiring system (fatty acid synthetase II, named by K. Bloch's group, 1969) and the acetyl-CoA-dependent ACP-non-requiring one (acetyl-CoA-dependent elongation of fatty acids reported by us, 1977). Now, a third fatty acid elongating system, malonyl-CoA-dependent and ACP-non-requiring, has been isolated from an extract of this microorganisms, separately from each of the previous two elongating systems. Primer specificity and cofactor requirements, especially of pyridine-nucleotide-coenzyme, of the last elongation system also distinguish it from the two previously known systems. All three systems, however, were found in a soluble fraction of M. smegmatis, therefore the regulatory mechanism for these elongation systems should be investigated hereafter.

Acyl Coenzyme A↗

Effects of arachidonic acid and bradykinin on the coronary flow, release of PGI2 and cardiac functions in the perfused guinea-pig heart.

The contribution of prostacyclin (PGI2) to the coronary vasodilating action of arachidonic acid (AA) and bradykinin (BK) was examined in isolated perfused guinea-pig hearts. The injection of Aa (100 to 1,000 ng) and BK (1 to 100 ng) into the heart resulted in a dose-dependent increase in the total amount of coronary flow and a release of 6-keto-prostaglandin F1alpha, a stable metabolite of PGI2. Both AA and BK showed weak positive chronotropic effects. In addition, higher doses (300 and 1,000 ng) of AA caused a transient reduction in the coronary flow rate, left ventricular systolic pressure, and left ventricular dp/dt. The changes in coronary flow, release of PGI2, and all cardiodynamic parameters induced by AA were abolished by pretreatment of the preparation with diclofenac-Na. On the other hand, the BK-induced increase in coronary flow rate was only partially reduced by diclofenac-Na when the release of 6-keto-prostaglandin F1alpha was completely inhibited. It is concluded that in isolated perfused guinea-pig hearts, BK has both PGI2-independent and PGI-2-dependent coronary vasodilating actions; the latter action is less than 25%, and the coronary vasodilating action of AA is mainly mediated via PGI2.

Animals↗

[Clinical evaluation of chemotherapeutic agents in the treatment of primary liver cancer].

For the comparison of effect of FT-207 alone and FT-207 + MMC combination chemotherapy on primary hepatocellular carcinoma, 41 patients were entered into the trial. Thirty-two cases whose data were evaluable were studied by Koyama's classification. There was only one effective case (6.6%) in single agent group (15 cases) and 5 cases (29.4%) were effective in combination chemotherapy (17 cases). MMC combination chemotherapy was more effective on regression in size of hepatomegaly than FT-207 alone. By osaka group classification, 5 cases (33.3%) in single agent group (15 cases) were effective, and 10 cases in combination group (17 cases) (58.8%) were effective. The slight side effect often necessitated upon cessation of the treatment in 31 cases but the disappeared on cessation or by decrease of dose.

Adult↗

[Concentration of cefotiam in the human bone marrow fluid and bone tissue (author's transl)].

The preoperative administration of cefotiam (CTM) 1 g by intravenous drip infusion for 30 minutes was performed. And the concentration of CTM in peripheral blood, bone marrow fluid and bone tissue was investigated. CTM was smoothly transmigrated from blood to bone tissue, and kept on the concentration in bone tissue over MIC values for several hours. These preliminary results encourage us to think that CTM may be as useful in prophylaxis against postoperative infections.

Adult↗

[Distribution of 67Ga-citrate in 3'-methyl-4-dimethylaminoazobenzene-induced hepatoma of rats].

The distribution of 67Ga-citrate in the hepatoma of rat induced by 3'-methyl-4-dimethylaminoazobenzene was studied. 67Ga uptake ratio resected specimen, autoradiography and histological specimen were compared each other. 67Ga uptake ratio of the tumor was increased 1.6 to 7.2 times (average 4.4) to control group. Regardless of the size of the tumor, macroautoradiographically observed distribution of 67Ga-citrate in the hepatoma was higher in the peripheral zone than in the central zone. Histologically the degeneration of tumor cell was low or absent in the peripheral zone of tumor, whereas it was intense in the central zone. 67Ga-citrate was highly accumulated in the zone which the degeneration was low or absent. We, however, could not demonstrate the site where 67Ga-citrate was incorporated.

Animals↗

[Concentration of dibekacin sulfate in bone (author's transl)].

The concentrations in serum, cancellous and cortical bones of dibekacin sulfate were measured in 16 cases. Dibekacin sulfate rapidly penetrates into bone. The peak concentration in serum and cancellous bone is reached within 1 hour after intramuscular injection and the concentration in cancellous bone parallels that in serum. The concentration in cortical bone increases with time. Cancellous bone has higher concentration than cortical bone. Dibekacin sulfate is suitable for prevention of infection in orthopaedic surgery, because it maintains a bactericidal level in bone for enough time.

Adult↗