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Biomedical subjects

S K Sharma

Publications and source records attributed to S K Sharma.

At least 613 records · Page 34Linked to original sources
PubMed1979

In vivo effect of endrin on three phosphatases in kidney and liver of the fish Ophiocephalus punctatus.

The effect of endrin (0.030 ppm) on the acid phosphatase, alkaline phosphatase and glucose-6-phosphatase activities in the kidney and liver of a teleost fish, Ophiocephalus (Channa) punctatus has been studied. The period of exposure was twenty days. An increase in the acid phosphatase activity is noted in the two tissues examined here. There is no significant change in the activity of alkaline phosphatase in kidney while in liver the enzyme is inhibited. In the kidney glucose-6-phosphatase showed a slight increase in activity but it is inhibited in liver.

Acid Phosphatase↗
PubMed1979

Non-operative management of a pelvic hydatid cyst communicating with the bladder.

Two cases are reported of spontaneous rupture of pelvic hydatid cysts into the bladder, which were managed conservatively. The resulting communication between the bladder and the cyst was used advantageously for intravesical instillation of 20% saline to destroy the germinal layer of the hydatid cyst and daughter cysts. Sequential cystographic studies showed disappearance of the extrinsic pressure effect on the bladder as the daughter cysts were evacuated repeatedly per urethram after instillations. Spontaneous sealing off of the communication was documented and confirmed by cytoscopy in 1 case. A plea is made to adopt a non-operative approach in such cases, which permits intravesical and, hence, intracystic scolecidal agent instillation and achieves spontaneous evacuation of daughter cysts. Other factors encouraging conservative management whenever possible are the reportedly high recurrence rate of hydatid cysts postoperatively, the morbidity described with complete excision of pelvic and other hydatid cysts, and the inherent slow process of recurrence (between 5 and 20 years) after complete evacuation of daughter cysts.

Child↗
PubMed1979

Affinity chromatography of Ruta graveolens L. O-methyltransferases. Studies demonstrating the potential of the technique in the mechanistic investigation of O-methyltransferases.

Two discrete furanocoumarin (5- and 8-)O-methyltransferases and a caffeic acid 3-O-methyl-transferase from cell cultures of Ruta graveoleus L. have been copurified by affinity chromatography on 1,6-diaminohexane agarose (AH-Sepharose 4B) linked with S-adenosyl-L-homocysteine (SAH). The furanocoumarin O-methyltransferases, which transfer a methyl group from S-adenosyl-L-methionine (SAM) to the 5- or 8-hydroxyls of linear furanocoumarins, were not retarded by 5-(3-carboxypropanamido)-xanthotoxin (CPAX) immobilized to AH-Sepharose 4B, but addition of SAM to the irrigant buffer led to complete retardation of both enzymes on this affinity system. An analogous phenomenon was observed for the caffeic acid O-methyltransferase, with a ferulic acid ligand coupled to the same insoluble support. SAH was as effective as SAM in promoting binding of the furanocoumarin O-methyltransferases to CPAX and caffeic acid 3-O-methyltransferase to immobilized ferulic acid, respectively. The strong and specific adsorption of these enzymes was abolished by exclusion of SAM or SAH from the irrigant buffer. It is concluded that the enzymes bind first to SAM or SAH, and that this binding process in turn induces the binding site for their specific phenolic substrates or their analogs. Based on these findings, a compulsory-ordered kinetic mechanism for the action of these O-methyltransferases is postulated.

Cells, Cultured↗
PubMed1979

Vaginocystography in vesicovaginal fistulae.

The importance of vaginocystography as an additional tool to excretory urography and cystoscopy in assessment of vesicovaginal fistulae is emphasized. A simple technique applicable even to large and multiple fistulae or a capacious vagina is described and discussed.

Female↗
PubMed1978

On the activation of bovine chymotrypsinogen A. Preparation of alanine-neochymotrypsinogen and its activation to alpha-chymotrypsin.

Alanine-neochymotrypsinogen was prepared by incubating 20 parts bovine pancreas chymotrypsinogen A with one part alpha-chymotrypsin in a solution containing 1 M (NH4)2SO4, 0.1 M sodium acetate, 0.05 M Tris buffer (pH 8.0) and 0.5 mg/ml soybean trypsin inhibitor. Optimal yields of NH2-terminal alanine were obtained after 60 h incubation at 4 degrees C. Ala-neochymotrypsinogen was isolated from the reaction mixture by affinity chromatography and ion-exchange chromatography on carboxymethyl-cellulose. As expected, the purified preparation was enzymatically inactive and, compared to chymotrypsinogen, had one additional NH2-terminal group identified as alanine. Ala-neochymotrypsinogen was activated by incubating with trypsin at a zymogen : trypsin ratio of 30 : 1 in 0.1 M phosphate buffer, pH 7.6 at 4 degrees C for 1 h. The fully active, stable species was identified as alpha-chymotrypsin.

Alanine↗
PubMed1978

The effect of in vivo exposure of endrin on the activities of acid, alkaline and glucose-6-phosphatases in liver and kidney of Ophiocephalus (Channa) punctatus.

The effect of in vivo exposure of a sublethal concentration (0.01 mg/l) of endrin on the activities of acid, alkaline and glucose-6-phosphatases in the liver and kidney of Ophiccephalus punctatus was studied. The period of exposure was twenty days. In the liver, alkaline phosphatase and glucose-6-phosphatase activities were decreased but acid phosphatase was stimulated. Kidney showed stimulation in the activity of all the three phosphatases.

Acid Phosphatase↗