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Biomedical subjects

S K Sharma

Publications and source records attributed to S K Sharma.

At least 595 records · Page 33Linked to original sources
PubMed1980

Affinity chromatography of cells and cell membranes.

This article attempts to introduce the concept of cellular affinity chromatography as it departs from molecular affinity chromatography. Special emphasis is placed on the selectio of a solid support as well as on the role of lectins as affinity ligands. Our major goal was to bring to light the basic principles involved, multiple options of ligands and matrices, and different techniques, which may be applied to separate the complex cell population as well as cell membranes. It is hoped that further developments in the field, especially in the selection of proper experimental conditions, ligands and matrix material may provide better results. We have tried to identify some of the potential problems which should be considered before these approaches can be used on a routine basis. Although the review deals primarily with the affinity chromatography of cells and cell membranes, examples are presented for diverse systems such as cell organelles, viruses and phages. A table summarizing the use of cellular affinity chromatography is included. It lists more than 80 examples covering the literature up to December, 1979.

Animals↗
PubMed1980

Ureterocalycostomy for postpyelolithotomy stenosis after failure of intubated infundibuloplasty.

Extended pyelolithotomy for staghorn calculus in a uremic patient with solitary functioning kidney was followed by development of stenosis of the infundibulum draining the superior and middle calyces. Drainage of the lower calyx down the ureter remained intact. Intubated infundibuloplasty proved unsuccessful since scarring and occlusion promptly recurred. Anastomosis of the middle calyx to the ureter was then performed with good results. We believe this is the first reported case of ureterocalycostomy for providing drainage to the middle calyx without lower polar nephrectomy.

Adult↗
PubMed1980

Ureteric diverticula: ureterodiverticular reflux and yo-yo effect. A television study with spot filming.

Two cases of ureteric diverticulum are presented. Repeated infective episodes were the main presenting features in one, whereas intermittent attacks of colicky pain brought the other patient in for clinical assessment. Fluoroscopic evaluation of the latter patient demonstrated free ureterodiverticular reflux and yo-yo phenomenon. Following diverticulectomy the pain completely disappeared.

Adolescent↗
PubMed1980

Site of Tc-99m binding to the red blood cell: concise communication.

The increasing interest in the use of Tc-99m-labeled red blood cells encourages exploration into the site and mechanism of binding of Tc-99m to the RBC components. The components isolated from the labeled red cells were the lipid and protein of the membrane, heme, and the alpha and beta chains of the globin. The binding occurs mainly to the protein moiety, and preferentially to the beta chain of the globin, where the specific activity was found to be 28 +/- 6 times that of the alpha chain. The mechanism of binding is discussed. The role of heme in the binding is not clear.

Binding Sites↗