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Biomedical subjects

S Inoue

Publications and source records attributed to S Inoue.

At least 1,585 records · Page 88Linked to original sources
PubMed1980

Purification of an anemia-inducing factor from human placenta and its application to diagnosis of malignant neoplasms.

An anemia-inducing substance was isolated from the human placenta and designated placental anemia-inducing factor (PAIF). An amount of 2.8 mg was purified from about 70 total placentas (total wet weight, 42 kg). PAIF caused hemolysis of human erythrocytes in vitro and reduced the number of erythrocytes in rabbits to 80% of that in the control by i.v. administration of 27 micrograms/kg of body weight. PAIF is a glycoprotein with a molecular weight of about 20,000 and containing about 56% sugar. Radioimmunoassay using 125I-labeled PAIF and antiserum revealed a common antigenicity with crude anemia-inducing substance in sera from patients with cancer. No cross-reactions were observed between PAIF and alpha-fetoprotein, carcinoembryonic antigen, Australia antigen, human chorionic gonadotropin, or human placental lactogen. The level of anemia-inducing factor in normal serum was less than 100 ng/ml, whereas 43 of 63 cancer patients (68.3%) had over 100 ng/ml of serum anemia-inducing factor. Anemia-inducing factor assay may provide a means of detection and aid in the treatment of malignant diseases.

Anemia↗
PubMed1980

The effect of cigarette smoke on the permeability of guinea pig airways.

The epithelium lining the conducting airways restricts the movement of inhaled foreign materials into the interstitial and circulatory space. In the present study, we have investigated the effects of graded doses of inhaled whole cigarette smoke on this function by measuring the rate of movement of horseradish peroxidase (HRP) from the airways lumen across the tracheal epithelium into blood, by assessing the penetration of HRP into tracheal epithelium using transmission electron microscopy, and by examining the morphology of tracheal epithelial tight junctions using the freeze-fracture technique. These studies showed that 5, 20, and 100 puffs of whole smoke increased penetration of HRP from the tracheal lumen into blood in a dose-dependent fashion. Transmission electron microscopy showed that HRP penetrated the tracheal epithelium only in the 100-puff exposure group while the freeze-fracture studies showed progressive disruption of epithelial tight junction beginning with a 100-puff exposure and becoming more extensive after the 200-puff exposure. We conclude that inhaled cigarette smoke damages the mucosal barrier and causes increased permeability to HRP by disrupting the intercellular tight junctions and that measuring the appearance of HRP in blood is more sensitive than electron microscopy in assessing this damage.

Airway Resistance↗
PubMed1980

[Painful hip joint with epiphyseal dysplasia (author's transl)].

Among patients who visited our hip clinic, eighteen patients from ten families were diagnosed as mild or atypical cases of spondyloepiphyseal dysplasia or multiple epiphyseal dysplasia. Clinical and X-ray examinations of these patients showed common characteristic features as follows. 1. They usually had short stature but were not dwarfs. Their facial features and body proportions did not suggest hereditary bone dysplasia. 2. Although the disease might be transmitted in an autosomal dominant manner, its occurrence among these families was found to be sporadic. 3. Symptoms of the hip joint mainly resulted from limited range of motion, coxalgia being usually less than expected from X-ray findings. 4. Joints were always affected bilaterally although the degree might be different on each side. 5. In the majority of the cases, the spine, shoulder (in 7 cases), knee (in 6 cases), elbow (in 3 cases), ankle, and wrist joint (in 2 cases) were also affected. 6. Characteristic X-ray findings of the hip joints were as follows. a) Coxa vara, flattening or rectanglar deformity of the femoral head and shortening of the femoral neck. b) The trabecular pattern of the femoral head was irregular and subchondral cysts were often observed. c) Joint space was relatively better restored than expected from the deformity and subchondral bony changes of the femoral head. Spondyloepiphyseal dysplasia and multiple epiphyseal dysplasia are genetic disorders of the epiphyseal and apophyseal cartilage. The hip joint is loading greater mechanical force than any other joint. In both spondyloepiphyseal dysplasia and multiple epiphyseal dysplasia, the hip joint is always affected. As the hip joint is most vulnerable when there is some disorder of the epiphyseal cartilage, it is probably the only detectable abnormal joint. In relatively short period (1972-1978), the diagnosis of mild or atypical spondyloepiphyseal dysplasia was made in 18 patients in our hip clinic. This means that the mild or atypical cases of spondyloepiphyseal dysplasia is not so rare as expected. They might be misdiagnosed as aseptic necrosis, deformity of the femoral head due to Perthes disease or primary osteoarthrosis. An extreme care should be taken when we examine such patients.

Adult↗
PubMed1979

[C1q deviation test].

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Antigen-Antibody Complex↗
PubMed1979

Purification and characterization of two lectins from Aloe arborescens Mill.

Two lectins have been isolated from leaves of Aloe arborescens Mill by salt precipitation, pH-dependent fractionation and gel filtration. One lectin (P-2) has a molecular weight of approximately 18,000, consists of two subunits (alphabeta) and contains more than 18% by weight of neutral carbohydrate. The smaller subunit (alpha) has a molecular weight of approximately 7,500 and the larger subunit (beta) a molecular weight of approximately 10,500. The other lectin (S-1) has a molecular weight of approximately 24,000, consists of two subunits (gamma2) with a molecular weight of approximately 12,000 and contains more than 50% by weight of neutral carbohydrate. An interesting feature of the amino acid compositions of these lectins is the high proportion of acidic amino acids, such as aspartic acid and glutamic acid, and the low proportion of methionine and histidine. S-1 has a strong hemagglutinating activity. On the other hand, P-2 has not only hemagglutinating activity but also mitogenic activity on lymphocytes, precipitate-forming reactivity with serum proteins, one of which is alpha2-macroglobulin, and complement C3 activating activity via the alternate pathway.

Aloe↗