Search PubMed⌕ Search

Biomedical subjects

S Hino

Publications and source records attributed to S Hino.

At least 127 records · Page 7Linked to original sources
PubMed1984

Transfusion-mediated spread of the human T-cell leukemia virus in chronic hemodialysis patients in a heavily endemic area, Nagasaki.

The incidence of anti-adult T-cell leukemia-associated antigens (ATLA) was surveyed in 134 patients under chronic hemodialysis in the Nagasaki area, as well as 4708 blood donors resident in the same area as controls: 23 patients (17%) and 201 donors (4.3%) were positive for anti-ATLA antibody. All seropositive patients were found to have had a positive history of blood transfusions. Seroconversions were confirmed in 9 cases, all of them after initiation of transfusions. In total, 216 units of blood were transfused in 7 seroconverted patients, and this is consistent with the estimated rate of anti-ATLA antibody-positive donor blood supplied by the Red Cross Nagasaki Blood Center (5%).

Adult↗
PubMed1984

Collison 6-jet nebulizer: choice for the certification test of the class II biological safety cabinet.

Three types of nebulizers, one plastic, one glass and Collison 6-jet nebulizers were tested, to select an appropriate nebulizer for the certification of the class II biological safety cabinets (BSC). Performance of the plastic and glass refluxing nebulizers were found to be both inconsistent from one to another, and not provide reproducible results. The Collison nebulizers, on the other hand, showed little variations and provided reproducible results. In addition, the Collison nebulizer generated constant spray volume when the fluid level in the reservoir was varied. To determine optimum discharge velocity of the Collison nebulizer, nebulizers with an outlet diameter of 10, 12, 14, 16 and 18 mm were tested. For the personnel protection test, the main stream of aerosol sprayed from the Collison nebulizer with 14 mm outlet internal diameter hit the intersection of the test cylinder and the front opening. Each nebulizer with an outlet diameter of 12 to 16 mm located at the level of lower edge of the front window delivered aerosols more efficiently at the intersection than when located at 14 inches above the work surface. The aerosol from the 10 or 18 mm diameter nebulizers showed an inappropriate distribution. For the product protection test, the nebulizer with 14 mm outlet diameter showed the best distribution pattern around the test cylinder. In the cross contamination test, this nebulizer outperformed the 10 or 18 mm for class II BSC's without side wall effect. According to these results, the Collison nebulizer with 14 mm of internal diameter nozzle was found appropriate for the biological certification tests of the class II BSC. Exception was the cross contamination test of class II BSC with a side air intake between the internal side wall and the work surface, in which the nebulizer with 10 mm nozzle was the choice.

Aerosols↗
PubMed1984

Reevaluation of the biological certification test for the class II biological safety cabinets.

The biological collectors for the biological challenge tests of the class II biological safety cabinets (BSC) described in the National Sanitation Foundation Standard No. 49 (NSF 49) were reevaluated. These tests are designed to evaluate personnel and product protection, and cross contamination control capabilities of BSC's. In the personnel protection test, the sampling efficiency of the impingers was approximately 1/10 of that the slit-to-agar samplers located at the same position. In the personnel protection test, the collection efficiency of the top pair impingers was 90% less than that of the middle pair of impingers (see text for placement location). Depending on the construction of the cabinet, the ratio of spores collected in the middle and the lower pair of impingers, and in the slit samplers varied considerably, from 98:7 to 3:40. In the product protection and the cross contamination tests, the number of colonies collected in a column of agar plates decreased by factors of 1/10 to 1/30 by deviating one column from the outlet of the impinger. In the cross contamination test, the nebulizer located at the division point distributed spores approximately 100 mm further from the side wall than that located at the midpoint of the internal side wall. For the class II BSC which has the air intake between the side wall and the work plate, the Collison 6-jet nebulizer with an outlet diameter of 10 mm (discharge velocity at 0.7 kg/cm2 = 1.8 m/s) was the choice instead of that of 14 mm (0.8 m/s), since the discharge velocity of aerosol from the nebulizer strongly affected the result of the cross contamination test.

Aerosols↗
PubMed1981

Immobilization osteoporosis and active vitamin D: effect of active vitamin D analogs on the development of immobilization osteoporosis in rats.

The therapeutic effects of vitamin D analogs, 1,24(R)-dihydroxycholecalciferol [1,24(R)-(OH)2D3], 1,24(S)-dihydroxycholecalciferol [1,24-(S)(OH)2D3], and 1,25-dihydroxycholecalciferol [1,25(OH)2D3] on immobilization osteoporosis were studied in rats. The right hind limb was immobilized through application of a plaster cast following the section of the sciatic nerve. The left hind limb was intact. Vitamin D analogs were orally administered for 6 weeks at dose levels of 0.02 and 0.10 micrograms/kg/day, respectively. The mean lengths of the immobilized femurs were not significantly different from those of the intact femurs in all the experimental groups. In the immobilized femur of animals treated with 1,24(R)(OH)2D3, 0.10 micrograms/kg, dry and ash weights were heavier and calcium and phosphorus contents greater than those in the nontreated group. Furthermore, the amount of calcified bone mass and the cortical thickness of the femurs of the immobilized limb in 1,24(OH)2D3-treated animals were greater than those in the nontreated animals. Treatment with 1,25(OH)2D3 at 0.10 micrograms/kg caused an increase of the bone mass in both immobilized and intact femurs when compared with those of the control group. It was concluded that the administration of 1,24(R)(OH)2D3 diminished the effect of immobilization in the development of osteoporosis without any side effects.

Animals↗
PubMed1981

Intraparticulate localization of some peroxisomal enzymes related to fatty acid beta-oxidation.

Male Wistar rats were given a diet containing 0.05% (w/w) LK-903 (alpha-methyl-p-myristyroxycinnamic acid 1-monoglyceride) for 2 weeks. The activities of four hepatic peroxisomal enzymes involved in the fatty acyl-CoA beta-oxidizing system were determined. The activities of fatty acyl-CoA oxidase, crotonase, beta-hydroxybutyryl-CoA dehydrogenase and thiolase were all increased about three times by administration of LK-903. The intraparticulate localizations of the four enzymes were then investigated by treatment of the purified peroxisomes with Triton X-100, by sonication, and by sucrose-density-gradient centrifugation after Triton X-100 treatment. The results suggest that thiolase is localized in the matrix of peroxisomes, that crotonase and beta-hydroxybutyryl-CoA dehydrogenase are located in the core, and that all or at least part of fatty acyl-CoA oxidase is associated with the core, though its association is weak.

Acetyl-CoA C-Acetyltransferase↗
PubMed1978

Genetic recombination between mouse type C RNA viruses: a mechanism for endogenous viral gene amplification in mammalian cells.

A strategy based on the identification of type-specific antigenic determinants in the transitional products of gag (p15, p12, and p30 proteins), pol (reverse transcriptase), and env (gp70 glycoproteins) genes of mammalian type C viruses has been used to study genetic recombination between these RNA viruses. By this approach, recombinants involving exogenous and endogenous mouse type C viruses have been identified and genetically mapped. Analogous techniques have been applied to investigate the genetic relationships between different classes of endogenous virus that exist within the same mouse cells. Proteins of the inducible class of xenotropic virus were shown to exhibit extensive antigenic homology with the gag but not the env gene products of the ecotropic virus class. Instead, the env gene-coded glycoproteins of the inducible and noninducible xenotropic virus classes possessed striking antigenic relatedness. These results, as well as supporting findings from molecular hybridization, favor the concept that the inducible xenotropic virus of mouse cells arose by a recombinational mechanism involving the progenitors of the other two endogenous virus classes.

Animals↗
PubMed1978

Physico-chemical properties of mouse hepatitis virus (MHV-2) grown on DBT cell culture.

Some properties of a strain of mouse hepatitis virus, MHV-2, grown on DBT cells were determined using a plaque assay on the cells. Viral growth was not inhibited by the presence of actinomycin D or 5-iodo-2-deoxyuridine. MHV-2 was completely inactivated by ether, chloroform, sodium deoxycholate or beta-propiolactone, but showed a moderate resistance to trypsin. Heating at 56 C for 30 min did not completely abolish the virus infectivity. The virus was stable after heating at 50 C for 15 min in 1M-MgCl2 or 1M-MgSO4 as well as at 37 C for 60 min at pH 3.0 to 9.0. Infectivity was decreased to 1/100 and 1/400 after storing at 4 C for 30 days and 37 C for 24 hr, respectively. The virus passed through a 200-nm but not a 50-nm Sartorius membrane filter. The buoyant density of MHV-2 was 1.183 g/cm3 in sucrose gradient, and the fraction contained coronavirus-like particles measuring 70 to 130 nm in diameter. Survival rate was 10% after exposure to ultraviolet at 150 ergs/mm2. Freezing and thawing or sonication at 20 kc for 3 min did not affect the virus titer. No hemagglutinin was demonstrable with red blood cells of the chicken, Japanese quail, mouse, rat, hamster, guinea pig, sheep, bovine or human.

Cell Line↗