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Biomedical subjects

S Goto

Publications and source records attributed to S Goto.

At least 1,081 records · Page 60Linked to original sources
PubMed1981

Purification and characterization of RNase-inhibitor complex from rat reticulocytes.

During purification of the RNase-inhibitor complex from rat reticulocytes, two forms of the complex were separated by DEAE-Sephadex chromatography. One complex was purified 10,000-fold from the cell lysate, and the other 1,000-fold. Both complexes were shown to be composed of 1 mol of RNase and 1 mol of inhibitor. Reconstitution experiments revealed that the multiplicity of the complex was primarily due to differences in the RNase. The RNases derived from the two complexes differed in their sensitivities to metal ions and hemin.

Animals↗
PubMed1981

Relationship between pigment producibility and drug resistance in Serratia marcescens.

Among the clinical isolates of Serratia marcescens, non-pigmented cells appeared more frequently from pigmented, drug-resistant strains than from pigmented, drug-sensitive strains. Transfer of R plasmid from Escherichia coli to pigmented strains caused spontaneous loss of pigment producibility, whereas such spontaneous loss never occurred in fresh cultures of drug-sensitive strains. The non-pigmented strain was a better recipient of R plasmid from E. coli than was the pigmented strain. R plasmid was transferred from the non-pigmented strain to the pigmented strain at a higher frequency than from E. coli to the pigmented strain. The results of the present investigation suggest that transfer of R plasmid may be one of the reasons for the significant increase of non-pigmented, drug-resistant strains of S. marcescens in nature.

Anti-Bacterial Agents↗
PubMed1981

Further investigations on the binding of loop diuretics to serum proteins from patients with liver disease.

The binding of three loop diuretics, piretanide, bumetanide and furosemide, to serum proteins from patients with liver cirrhosis or fulminant hepatitis was investigated using equilibrium dialysis. A good correlation was found between serum albumin concentration and the percentage of each unbound (free) loop diuretic in patients with liver disease. The binding data obtained from patients with liver cirrhosis was compared with that of patients with chronic renal failure. Calculations made according to the Sandberg-Rosenthal's formula revealed that the maximum binding concentration (nP) varied in some cases. These findings necessitated a detailed investigation into whether the increased percentage of each unbound loop diuretic in patients with liver disease is attributable not only to lowered serum albumin concentration but also to inhibition of the protein binding by some endogenous substances. Thus, similar experiments were performed using rats with experimental liver cirrhosis. The binding of the loop diuretics to serum proteins in cirrhotic rats differed greatly from the findings obtained from cirrhotic patients. The percentage of unbound loop diuretic was well correlated with serum albumin concentration but not with the concentration of serum bilirubin (an endogenous substance) in cirrhotic rats.

Adult↗
PubMed1981

Studies of anaerobic bacteria in chronic otitis media.

In the 760 cases of chronic otitis media studied from 1970 to 1976, the detection ratio of anaerobic bacteria and the relation between the nature of anaerobic bacteria and the conditions of focus were investigated. In 8.2% of 760 cases 9 kinds and 81 strains of anaerobic bacteria was detected. Most of the anaerobic bacteria were Peptococcus sp. or Bacteroides sp. and in general non-sporogenic anaerobic bacteria accounted for 97.5%. Anaerobes were frequently detected in cases where the middle ear cavity was filled with cholesteatoma or granulation and in cases of infection recurring after operation.

Bacteroides Infections↗
PubMed1981

Specific enzyme immunoassay for human chorionic gonadotrophin.

A sandwich-type enzyme immunoassay system specific for human chorionic gonadotrophin (hCG) was prepared with the antibody Fab'-beta-D-galactosidase complex and the antibody F(ab')2-immobilized silicone rubber solid phase by using a purified antibody to beta subunit of hCG (hCG beta). The assay system cross-reacted less than 4% with human luteinizing hormone (hLH) and human follicular stimulating hormone (hFSH), and proved to be highly sensitive with hCG measurable at levels as low as 0.3 mIU per assay tube. Using 50 microliter of serum sample, 6-600 mIU/ml of hCG in serum could be determined specifically with the same degree of precision as in radioimmunoassay but without sample interference with the assay. The coefficients of variation within-run and between-run were 8.6-8.9%, and 4.9-10.7%, respectively. Values obtained with the enzyme immunoassay correlated well with those of radioimmunoassay (r = 0.98, slope = 0.94, y-intercept = 10.2 mIU/ml for 75 serum samples). Results of the immunoassay of hCG levels in serial samples of serum from healthy women and patients with choriocarcinoma show that this method is useful in the clinical diagnosis of trophoblastic disease.

Chorionic Gonadotropin↗
PubMed1981

High performance liquid chromatographic determination of aclacinomycin A and its related compounds. I. Normal phase HPLC for monitoring fermentation and purification processes.

A normal phase high performance liquid chromatographic method is presented for the separation and quantitative determination of aclacinomycin A (ACM) and its related compounds classified as anthracycline antibiotics. The sensitive, reliable and reproducible chromatography was achieved on a mu-Porasil column with a mobile phase consisting of chloroform-methanol-acetic acid-water-triethylamine (68:20:10:2:0.01, v/v). By use of MA144 M1, one of the ACM analogues, as an internal standard, selectivity of the HPLC method could be retained by virtue of the adjustment of the detecting wavelength to 436 nm. The HPLC method was applicable to monitoring the fermentation and purification processes.

Aclarubicin↗
PubMed1981

High performance liquid chromatographic determination of aclacinomycin a and its related compounds. II. Reverse phase HPLC determination of aclacinomycin A and its metabolites in biological fluids using fluorescence detection.

A sensitive, simple and selective reverse phase high performance liquid chromatographic method was applied to the determination of aclacinomycin A (ACM) and its metabolites in plasma. The chromatography was performed on a microparticulate phenethyl derivative of silica using a mobile phase of acetonitrile-0.03 M ammonium formate buffer (pH 5.0), (50:50, v/v) and monitoring with fluorescence detector (excitation 435 nm-emission 505 nm). High reproducibility and linear calibration curves were obtained from 20 ng/ml to 100 ng/ml of ACM and its metabolites in plasma by use of internal standard methods.

Aclarubicin↗
PubMed1981

[[Studies of Ossification of the posterior longitudinal ligament in the cervical spine using microradiography and histochemistry (author's transl)].

ossification of the posterior longitudinal ligament (OPLL) is seen by X-ray as a condition which shows a heterotopically formed bony shadow at the posterior part of vertebral bodies of the cervical spine. OPLL causing severe myelopathy is called a Japanese disease. Recently, it has been shown that this condition is not only seen in Japan, but also in other countries especially in Asia. In order to obtain information of pathogenesis of this condition, the present author carried out histochemical investigations on 2 autopsy cases which had been treated as OPLL myelopathy, and 12 surgical materials. Totally 30 spinal vertebrae were examined. They were prepared for study with undemineralized ground sections mainly. The following results were obtained. 1) Microradiography revealed that the calcified tissue on the ligament had two marked structures, one was highly calcified tissue, and the other was bony tissue with lower density. 2) Most of the highly calcified tissue with variable distribution ranging from a sparse to a dense one contained round radiolucent spots probably consisting of cartilage-like cells. This type of calcified tissue is accompanied by the organic matrix, which was stainable with van Gieson stain, PAS reaction, Alcian blue stain and toluidin blue metachromasia. 3) A part of highly calcified tissue showed linearly distributed pattern without radiolucent round spots. This type of calcified tissue was also stained with van Gieson stain and PAS reaction. However, this organic matrix was not accompanied by marked reaction of acid mucopolysaccharides. 4) No essential histochemical difference was found between the highly calcified tissue on the ligament and other various types of heterotopically formed calcified tissue. 5) Microradiography revealed the remnant of resorption process at the boundary between the highly calcified tissue and the bony tissue. This finding of the resorption lacunae indicated that the highly calcified tissue was formed initially, followed by bone tissue formation. The process of internal remodeling was also observed in the bony tissue. 6) The distribution of the highly calcified tissue, bony tissue and resorption cavities indicated several patterns of remodeling process which was characterized by the new bone formation at the region near the vertebral body. These characteristics were apparently due to the mechanical stresses acting on this bony structures, as seen in some other heterotopic bone formations such as the healing process of fracture or tooth extraction socket.

Adult↗
PubMed1981

[Partial purification of bilirubin binding protein from the human term placenta (author's transl)].

Fetal bilirubin may pass through the placenta into the maternal circulation with yet unknown mechanisms and be metabolized in the maternal liver because of deficiency of bilirubin conjugating and excreting activity in the fetal liver. To elucidate bilirubin transport mechanisms in placenta we partially purified bilirubin binding protein from 105,000 X g supernatant of the villous homogenates from the human term placenta. The supernatant was brought to 50% saturation with ammonium sulfate. The precipitates dissolved in 0.25 M sucrose buffer (pH 7.4) were chromatographed on a Sepharose 6B column (2.6 X 91.5 cm) with the same buffer. Each fraction was analyzed for protein and bilirubin binding activity. The highest bilirubin binding activity was eluated at void volume. After rechromatography the void volume fraction was centrifuged in a linear sucrose density gradient. The 4.8S fraction with the highest bilirubin binding activity was then applied onto a Sepharose 6B column (0.9 X 58 cm) and rechromatographed with 0.4 M sucrose buffer. After rechromatography the most active fraction was analyzed using sodium dodecylsulfate-polyacrylamide gel disc electrophoresis. The molecular weight of the placental bilirubin binding protein was found to be about 76,000 both in Sepharose 6B gel filtration and gel electrophoresis. The results may indicate that the bilirubin binding protein was of single polypeptide chain distinct from ligandin, Z-protein, and alpha-fetoprotein.

Bilirubin↗