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Biomedical subjects

S Goto

Publications and source records attributed to S Goto.

At least 1,099 records · Page 61Linked to original sources
PubMed1980

Blood group Rh-D factor in human trophoblast determined by immunofluorescent method.

The chorionic villi of human placentas, 12 to 40 weeks' gestation, were examined for Rh-D blood group antigens with a direct immunofluorescent (IF) technique. Specific IF-standing was shown on the plasma membrane and cytoplasm of the villous trophoblasts. This staining decreased in intensity as the placenta matured. In addition, the trophoblasts of hydatidiform and destructive moles were also examined. The intensity of the IF-staining in these trophoblastic tumors was about the same as that in the young trophoblasts of the first trimester of pregnancy.

Fluorescent Antibody Technique↗
PubMed1980

Congenital cerebellar neuroepithelial tumor with multiple divergent differentiations.

A case of congenital cerebellar tumor is reported. The tumor is composed of a few incomplete tubular structures analogous to the neural tube, and of neuroblastic, mature ganglionic, astrocytic, ependymal and undetermined neuroepithelial cells. A tentative diagnosis of congenital cerebellar neuroepithelial tumor with multiple divergent differentiations is made: the histogenesis is discussed. From a histogenetic point of view this tumor is given the provisional name of matrix cell tumor.

Autopsy↗
PubMed1980

Postoperative acetylcholinesterase activity in Hirschsprung's disease.

Histochemical staining and biochemical assay of acetylcholinesterase (AChE) activity were applied to rectal suction biopsy tissue from patients treated by Z-shaped anastomosis for Hirschsprung's disease. Postoperative AChE activity and AChE/Total ChE (% AChE) in the rectal anterior wall were significantly higher (p < 0.001) than those in the posterior wall. Biochemical AChE activity was well correlative to visual mucosal AChE activity. It is remarkable that AChE activity in the rectal anterior wall after Z-shaped anastomosis was found to be kept at high level for a long period without any relation to the time interval after the operation, the ano-rectal function, and the recto-anal reflex.

Acetylcholinesterase↗
PubMed1980

Oral contraceptive steroids: effects on iron and zinc levels and on tryptophan pyrrolase and alkaline phosphatase activities in tissues of iron-deficient anemic rats.

Weanling Wistar-strain female rats were fed a normal or an iron-deficient diet for 8 weeks and oral contraceptive steroids (OCS) were added for the last 4 weeks. Hemoglobin content, serum iron and zinc levels, liver iron levels, and tryptophan pyrrolase activities, and liver, kidney, and brain zinc levels and alkaline phosphatase activities were determined. Compared to control rats given the normal diet (N group), elevated liver zinc levels and tryptophan pyrrolase activity were found in rats fed the normal diet containing OCS ( + S group), but other parameters did not alter. In rats fed the iron-deficient diet alone (D group), only liver zinc levels were significantly higher, while other parameters were in general lower than those in the N group. In rats fed the iron-deficient diet containing OCS (D + S group), all hematological values, tissue mineral contents with the exception of liver zinc levels, and liver tryptophan pyrrolase and kidney alkaline phosphatase activities were lowered, compared to the N or N + S group. However, compared to the D group, the values of most parameters in the D + S group did not differ significantly, apart from an increase in serum zinc levels. These observations suggest that OCS does not greatly influence the various changes caused by iron-deficient anemia.

Alkaline Phosphatase↗
PubMed1980

Steady-state kinetics of trypsin-catalyzed hydrolysis of a synthetic substrate, dansyl-D-arginine methyl ester.

The trypsin-catalyzed hydrolysis of a synthetic ester substrate, dansyl-D-arginine methyl ester (D-DAME), was followed by the entire progression curve method. This ester substrate, which is a D-enantiomer of arginine, is hydrolyzed to the extent of nearly the pH or the initial substrate concentration. The double-reciprocal plot of the velocity of hydrolysis against the residual substrate concentration showed good linearity. As regards the dependency of this straight line on pH at a constant initial substrate concentration, both the slope and the intercept on the 1/v axis decreased with decrease of hydrogen ion concentration in the pH range below 8 and the lines intersected at a point in the second quadrant. At constant pH the slope of the double-reciprocal plot between the velocity of hydrolysis and the residual substrate concentration increased linearly with increase of the initial substrate concentration. The results obtained cannot be interpreted simply in terms of the three-step mechanism for the trypsin- and chymotrypsin-catalyzed hydrolyses of ester substrates. In particular, it is significant that the catalytic rate constant obtained for the overall enzymatic reaction is very similar to the rate constant of conformational change of the enzyme molecule due to the formation of enzyme-substrate complex in the trypsin-catalyzed hydrolyses of L- and D-DAME.

Animals↗
PubMed1980

Inhibitory effect of Pseudomonas aeruginosa on phagocytosis and killing of rabbit polymorphonuclear leukocytes.

Polymorphonuclear leukocyte (PMN)-resistant strains of Pseudomonas aeruginosa had the ability to interfere with phagocytosis of Escherichia coli, whereas PMN-sensitive strains of P. aeruginosa did not. When mice were infected with an ordinarily nonpathogenic strain of E. coli, addition of a PMN-resistant strain of P. aeruginosa gave a mortality considerably higher than that obtained with P. aeruginosa alone, whereas addition of a PMN-sensitive strain of P. aeruginosa gave a mortality not different from that observed with P. aeruginosa alone. This increased mortality in mixed infection with E. coli and PMN-resistant P. aeruginosa was obtained by facilitation of tissue invasion by both bacteria from the inoculum site.

Animals↗
PubMed1980

Binding of several loop diuretics to serum albumin and human serum from patients with renal failure and liver disease.

The binding of the new diuretic piretanide and two other diuretics (furosemide and bumetanide) to serum proteins (especially albumin) was studied using the in vitro equilibrium dialysis method. Piretanide and bumetanide, similar to each other in chemical structure, were almost equal in the binding constant to purified albumin, while the binding constant of furosemide was greater than that of the other two diuretics. The binding of these loop diuretics in the dilutions of the sera from healthy adults and patients with renal failure or liver disease was also studied by the same method. Protein binding of the drugs was markedly reduced in patients with renal failure. The cause could only partly be explained by some structural changes in the serum albumin molecules of these patients and influences of endogeneous substances present in serum.

Adult↗
PubMed1980

In vitro and in vivo antibacterial activities of FR-31564, a new phosphonic acid antibiotic.

FR-31564, a new phosphonic acid antibiotic, was active against most Gram-negative bacteria except Serratia marcescens and glucose-nonfermenting Gram-negative rods excluding Pseudomonas aeruginosa. The antibacterial activity in vitro of FR-31564 was stronger than that of fosfomycin especially against Escherichia coli, Klebsiella pneumoniae, Enterobacter species and P. aeruginosa. FR-31564 also was active against Gram-negative bacteria resistant to beta-lactam antibiotics and against gentamicin-resistant strains of P. aeruginosa. The antibacterial activity in vitro of FR-31564, like that of fosfomycin, was enhanced when 10% rabbit blood was added to the nutrient agar. The therapeutic efficacy of FR-31564 in experimental infections in mice was superior to that of fosfomycin in infections due to most Gram-negative bacteria used, and was similar to that of gentamicin in infections due to Citrobacter freundii, Proteus rettgeri and Proteus inconstans B. The protective effect of FR-31564, particularly in the P. aeruginosa infection, was superior to that of other control drugs including gentamicin.

Animals↗