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Biomedical subjects

S Georgiev

Publications and source records attributed to S Georgiev.

At least 37 records · Page 2Linked to original sources

[Microbial etiology of the MMA syndrome (mastitis-metritis-agalactia) in swine raised commercially].

A total of 147 uterine-cervical exudates and milk samples from 165 lobes of the mammary gland of sows with clinical symptoms of mastitis-metritis-agalactia (MMA) were studied microbiologically. It was found that greatest was the share of Escherichia coli and Staphylococcus aureus organisms taking part in the microbial etiology of the syndrome. Second ranked Staphylococcus epidermidis, Streptococcus pyogenes, and Corynebacterium pyogenes along with some aerobic sporulating bacilli.

Animal Husbandry↗

[Drug resistance of bacterial strains isolated from sows with the clinical picture of mastitis-metritis-agalactia].

The disk method with Ericson and Bauer's solid nutrient media were employed to ascertain the drug resistance of a total of 54 strains of Escherichia coli, 25 strains of Staphylococcus aureus, 11 strains of Streptococcus pyogenes, 7 strains of Corynebacterium pyogenes, 7 strains of Streptococcus uberis, 2 strains of Streptococcus agalactiae, and 1 strain of Streptococcus disgalactiae isolated from utero-cervical exudate and milk samples of sows with clinical symptoms of mastitis-metritis-agalactia (MMA). It was found that gentamycin only suppressed 100 per cent of the strains. So far as the other chemotherapeutic agents were concerned the organisms showed a varying extent of resistance. After determining the sensitivity of the strains to drugs two groups of pigs (from which the strains referred to were isolated) were subject to treatment. All 20 sows of the first group were treated with oxytocin only, while the 446 animals of the second group were treated with gentamycin in combination with oxytocin. It was found that effective therapy in the case of MMA was impossible with the application of preparations on the base of the hind lobe of the pituitary only. It is necessary to use chemotherapeutic means tow which the isolated organisms are susceptible.

Animals↗

[Clinical aspects and diagnosis of pulmonary edema in pulmonary thromboembolism].

Thirty six patients that died with pulmonary thromboembolism (PTE) were retrospectively followed up, whose diagnosis had been made both by the classical instrumental investigations and by angiopulmography and scintigraphy of the lungs. The diagnosis pulmonary edema in PTE was made on the base of the clinical picture, in which the characteristic pink expectoration was absent. ECG was with signs of acute pulmonary heart. The X-ray of the lungs shows, most frequently, right and diffuse interstitial pulmonary edema.

Cardiac Catheterization↗

[Use of Falicard and Sectral in patients with COPD and ischemic heart disease].

Thirty patients with chronic obstructive pulmonary disease (ChOPD) and concomitant ischemic heart disease (IHD) were studied that were admitted to the clinic for the treatment of a recurrence of the basic disease within the period 1981-1982. The patients were grouped into three groups of 10 patients and each group was treated for a period of 14 days, as follows: group 1--10 patients were given only sectral 50 mg per os during the first week and 10 mg/day during the second week; group II--treated with falicard, 5 mg daily, intravenously, for one week and later 2 X 5 mg, intravenously--the second week; group III, received sectral--50-100 mg/day after 5 mg falicard intravenously. Best results, manifested with a reduction of the paroxysms of dyspnea and stenocardia, were obtained in group III, suggesting the advantage of the combined effect with cardioselective beta-blockers and Ca-antagonists in the patients with ChOPD and concomitant IHD.

Angina Pectoris↗

[Pulmonary edema in pulmonary thromboembolism].

Thirty six decreased with pulmonary thrombembolism (PTE) were analytically studied. 18 of them being with concomitant pulmonary edema (PE), confirmed pathologoanatomically. The incidence established was in 50 per cent of all the deceased, most often in combination with the unfavourable--factors arterial hypertension IIB stage and ischemic heart disease in 77,7 per cent. The effect of two factors on hemodynamics is discussed--elevated peripheral vascular resistance and coronary ischemia. In 18 deceased patients with PTE and PE the multiple intrapulmonary localization of emboli (61%) was predominating as well as the advancement of right cardiac insufficiency.

Adult↗

[Trial of the therapeutic action of ultrashort waves in combination with chemotherapeutic agents and antibiotics in chronic endometritis in cows].

Ninety cows affected with Endometritis catarrhalis et purulenta chronica, were divided into three groups consisting of thirty cows each and were equalized according to the method of analyses, were treated for experimental purposes following three diagrams. First diagram (30 cows). On the first, third, fifth, seventh and ninth days trivitaminol with antibiotic (according to antibioticogram) was inserted through the uterus in a dose of 50 cm3, as well as 20 cm3 of Borgal intramuscularly. Second diagram. Thirty cows were treated only with ultrashort waves. The radiation treatment lasted 10 days and the duration of seances was respectively increased. Third diagram. Another group of thirty cows were treated in a combination of antibiotics, chemotherapeutic preparations and ultra-short waves. As a test, on the basis of which the general status of cows was traced out before and after the treatment, were taken the indices of total protein and protein fractions and the bactericidal activity of the serum, the phagocytic activity of leucocytes, the presence of "C" reactive protein in the serum and the total number of leucocytes and bacteria in the uterine secretion. It was established that the combined medicinal therapy of chronic endometritis in cows, under the effect of ultra-short waves, led to an increased effectiveness of the treatment. The multilateral effect of ultra-short waves reinforced the therapeutic activity of antibiotics and sulphonamides used during the treatment of endometritis. Treatment duration was cut down and fecundity increased.

Animals↗

[Reactivity changes in calves during transportation].

The activity of 38 calves aged 15-30 days of the Simenthal breed and of crosses with Ireshire was studied before and after transportaton at a distance of 38-100 km. It was established that transportation causes fatigue, thirst and a weight reduction of 4.3 kg, due to loss of liquid in the calf's organism. Caughing, soft faeces, even diarrhea, 1.5 degrees C higher rectal temperature, and 3.0 degrees C lower skin temperature, were recorded. the MacClure--Oldridge test proved twice as fast. As a result of hemoconcentration hemoglobin and hematocrite were higher. Eosinopaenia and neutrophylia with a nuclear deviation to the left similar to the reaction of experimentally induced lipopolysaccharide fever were established. On the 72d hour lymphocytosis and an enhanced lymhocytal index were observed. Transportation led to 15-18% higher number of phagocyted neutrophyls, 30-35% higher Wright number and nearly two times higher total blood phagocytal ability. Seventy two hours after two times higher total blood phagocytal ability. Seventy two hours after transportation the total blood phagocytal ability was reduced 15%. Another effect of calf transportation was the reduced total immunological reactivity, scored by the skin test of Yoffe.

Animals↗

[Resoprtion of colostric immunoglobulins and serum bactericidal activity of lambs during ontogeny].

Studied were the resorption dynamics of colostrum IgG and IgM immunoglobulins in lambs of ewes vaccinated and unvaccinated against Escherichia coli and the bactericidal activity of their serum depending on the time after the first sucking as well as the resorption and the bactericidal activity as influenced by the first sucking itself. The experiment was carried out on 70 lambs of vaccinated ewes and 30 lambs of unvaccinated ewes. The period of investigation covered the time from the first to the 20th day after the first sucking, studying also the agglutinin titer, the phagocyte number and phagocyte index of the leukocytes with regard to the vaccinal strain O7 of E. coli. The specificity of IgG and IgM was studied through immunoelectrophoresis. It was found that the bactericidal activity of the blood in newborn lambs was dependent on the amount of the immunoglobulins resorbed and their specificity. A delay of 10--24 hours of the first sucking led to a drop of both immunoglobulins and bactericidal activity by about 48--65 and 20--45 per cent, respectively.

Animals↗

[Acid-base equilibrium in mother and fetus during labor and the resistance of newborn calves].

Investigated were a total of 59 cows presenting normal processes, of giving birth as well as dystocia, the time of delivery lasting 2, 10, 24, and 36 hours. Seventeen cows with dystocia were treated intravenously with 500 cm3 of a 4 per cent solution of sodium bicarbonate and 40 g glucose. It was found that the changes in the pH values, alkali reserves, glycogen, and the peroxydase activity of the neutrophiles in the mothers and fetuses were dependent on the duration of parturition. Cases of difficult labour with a prolonged calving period led to lowering the pH values the alkali reserves, and the enzyme activity of leukocytes both in the cows and the newborn calves. Newborns delivered 24 and 36 hours from the beginning of the parturition act showed low resistance and had weakly expressed phagocytic and bactericidal blood activity. The intravenous application of 50 ml of a 4% solution of sodium bicarbonate and 40 g glucose to cows with dystocia up to the 10th day led to a compensatory response on behalf of the acid-base balance in the calves.

Acid-Base Equilibrium↗

[Agammaglobulinemia and hypogammaglobulinemia in newborn lambs depending on the time of the first suckling].

Studied were the agammaglobulinemia and the hypogammaglobulinemia in 120 newborn lambs of which 100 had sucked for the first time at the first hour following birth, 10 had sucked at the ninth hour, and the remaining 10--at the 24th hour. Blood was sampled at the 1st, 2nd, 3rd, 5th, 10th, 24th, and 48th hour, and was immunoelectrophoretically studied after Grabar. Precipitation was done with hyperimmune antiovine gammaglobulin serum. Part of the lambs with agammaglobulinemia were killed and the small intestine was used to prepare extracts for an immunoelectrophoretic study after Grabar and for establishing gammaglobulins. It was found that lambs that had first sucked at the 1st hour following birth the resorption of the colostral gammaglobulin at the various hours later on varied. In the blood serum of 40% of the investigated lambs at the 1st hour there were gammaglobulins; gammaglobulins in 96% of the animals at the 24th hour after they had first sucked. A delayed first sucking after birth--at the 10th and 24th hour--led to hypo- and agammaglobulinemia. In the blood of such lambs at the 48th hour after sucking the gammaglobulins were 0.70 and 0.95 g% less as against the amount of gammaglobulins in lambs that had sucked for the first time at the first hour after birth.

Agammaglobulinemia↗

[Sheep placenta water-soluble proteins].

Immunoelectrophoretic investigations were carried out with water-sault extractions from the placentae of 100 in-lamb ewes manifesting normal pregnancy, studying 20 animals during each month of pregnancy. The placentae were taken immediately after slaughter. The same investigations were performed also on a total of 40 placentae of ewes that had miscarried. Precipitation was carried out with hyperimmune sera against placental protein of the first and fifth month of pregnancy. Results showed that the placenta can synthetize proteins. In the first two months of pregnancy protein synthesis is at a lower stage, but in the 3rd, 4th and 5th month it becomes more intense. Each month's proteins are characterized by a specific immunoelectrophoretic picture. At the end of the first month as many as four fractions are established electrophoretically in the placental proteins, which, by the end of the pregnancy period rise up to ten. In the course of pregnancy, along with the organospecific proteins stage-specific ones are also synthetized. These can be demonstrated through active anaphylaxis in guinea pigs. Abortions in ewes are said to disturb the normal synthesis of proteins. The "immunoelectrophoregrams" of ewes that have miscarried in the 3rd, 4th and 5th month of pregnancy reveal the drop in the intensity of the albumin fraction and the number of arcs (from 10 to 5) in the section of the alpha, beta and gamma globulins.

Abortion, Veterinary↗

[Immunoelectrophoretic studies in Marek's disease].

Immunoelectrophoretic studies were carried out with the sera obtained from 10 chickens affected with acute Marek's disease. The diagnosis was confirmed clinically, morphologically, and via the embryonic test of von Bulow. The blood sera of ten normal hens served as controls. Results showed that in the sera of the diseased chickens there were changes in the protein spectrum, whereas the alfa and gamma-globulin fractions were divided into three subfractions. The exhaustion of the diseased sera with hyperimmune sera against sera of normal chickens revealed characteristic alfa2-globulins. The results obtained were confirmed with the active anaphylaxis in guinea-pigs.

Animals↗