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Biomedical subjects

S Georgiev

Publications and source records attributed to S Georgiev.

At least 19 recordsLinked to original sources
PubMed2005

High-resolution ultraviolet spectroscopy of p-fluorostyrene-water: evidence for a sigma-type hydrogen-bonded dimer.

Ab initio calculations predict four stable conformational structures of the singly hydrated cluster of p-fluorostyrene: two out of plane with pi- and two in plane with sigma-type intermolecular hydrogen bonding between p-fluorostyrene and water. We employed mass-selective resonance-enhanced two-photon ionization high-resolution (70-MHz FWHM laser bandwidth) spectroscopy to partially resolve the rotational structure of the 0(0) (0) origin band of the S(1) <--S(0) electronic transition. A computer-aided fit based on genetic algorithms was used to analyze the experimental high-resolution spectrum and to determine the observed conformational structure. The good agreement between the experimental and the simulated spectra of the 0(0) (0) band and the assignment of the other prominent bands as inter- and intramolecular vibrational progressions clearly demonstrates that the anti in-plane conformer is the most abundant one in the molecular beam. The existence of the sigma-type hydrogen bond between p-fluorostyrene and water manifests that the electron attracting effect of fluorine dominates over the releasing mesomeric effect of the vinyl group and thus a pi-type hydrogen bonding with the aromatic ring is not favored in this case.

Journal Article↗
PubMed2004

Spontaneous chromosome doubling results from nuclear fusion during in vitro maize induced microspore embryogenesis.

A multidisciplinary study was carried out to analyse the chromosome doubling process during the early stages of in vitro maize microspore embryogenesis. The main stages (microspore derivatives) that were formed in the course of the culture were analysed. Chromosome number was determined from squashed cells, and DNA content was measured by cytometry. In parallel, an ultrastructural analysis of the microspore derivatives demonstrated the occurrence of a nuclear fusion process. It seems likely that nuclear fusion ensures chromosome doubling at early stages of induced microspore embryogenesis. It occurs precisely at the 5/7 day stage in the embryonic domain and probably leads to polyploidy in the endosperm domain of the microspore derivatives. As a conclusion a scheme summarises the results and proposes an interpretation of the sequence of chromosome doubling events during early maize microspore embryogenesis. Understanding of this process will be important for future efforts to increase the percentage of homozygous plants for crop improvement.

Cell Fusion↗
PubMed2004

Mass analyzed threshold ionization spectroscopy of p-fluorostyrene.

Adiabatic ionization energy (AIE) and two-color threshold ion vibrational spectra of p-fluorostyrene have been measured by mass analyzed threshold ionization (MATI) method via three different intermediate levels in the first excited state, vibrationless S1 origin, 42(1)41(1), and 23(1) vibronic levels. Features of the ion vibrational spectra indicates that the geometry of the molecular ion including the conformation of the vinyl chain in the ionic ground state (D0) is almost identical to that of its neutral ground state (S0), and ionization has very little effect on the vibrational potentials of the aromatic ring modes. Comparison of the AIE with the reported value of styrene shows that fluorination at the para position of the aromatic ring has little effect on energy of the electron ejected in ionization process from the styrene chromophore.

Journal Article↗
PubMed2004

[Respiratory and hemodynamic monitoring during laparoscopy and two computer-controlled TCI anesthesiologic technique].

Forty women under general anaesthesia (computer-controlled TCI-infusion) in two groups were subjected to non-invasive haemodynamic monitoring together with arterial gasometry and capnography. Simultaneous continuous monitoring of aortic blood flow and PetCO2 allows an undelayed recognition of major circulatory disturbances, before significant changes in heart rate and arterial pressure occur.

Anesthesia, Intravenous↗
PubMed2003

[Some opportunities for the application of the drug mycosyst (preliminary communication)].

The colpitis is one of the most frequent gynaecological disease. The different methods of treatment of colpitis are a matter of discussion in many specialized studies. The aim of our retrospective study was to evaluate the therapeutic effects of Mycosyst (Fluconazol), produced by the pharmaceutical company Gedeon Richter (Budapest, Hungary), on women with acute fungal colpitis. The experience dated from about eight months (April--December 2002) with 27 patient with anamnestic, clinical, microscopic and microbiological data of fungal colpitis. Women were divided into two groups: 16 patients with diagnosed fungal colpitis at the time of interruption of pregnancy and 11 patients with this complaints not related to pregnancy. The therapeutic scheme is 150 mg Mycosyst as a single dose. The subjective complaints began to disappear 48 hours after the administration and were completely eliminated in almost all cases four days later. The medicine has a very fast effect on pruritus, erythema and urinary complaints, and a little bit slower influences the vaginal fluor. Some good pharmacodynamic and pharmacokinetic parameters of Mycosyst are: fast and complete absorption in gastrointestinal tract, long plasma life, elimination by the kidneys without being metabolized, doesn't affect the plasma concentration of steroids and testosterone, high and long terming concentration in each stratum of skin, nails, vaginal secretion and saliva. Our conclusion is that Mycosyst is reliable, easily applied broad-spectrum azol antifungal. The single dose of 150 mg is sufficient for treatment of acute fungal colpitis in almost 90%. The medicine is comparatively cheap and accessible.

Adult↗
PubMed2001

Transcriptional activity of an inversion split NOR in barley (Hordeum vulgare L.).

The transcriptional activity of NORs in a new structural mutant of barley (PK-88-4) was studied based on the positive reaction of NORs of metaphase chromosomes with AgNO3 and the number and size of silver-stained nucleoli in somatic interphase cells and meiocytes. PK-88-4 proved to contain a pericentric inversion that split NOR 7 into two unequal parts residing in the opposite arms of the reconstructed chromosome 7(5). This chromosomal rearrangement enables testing of intrachromosomal suppression of NORs and provides insight into the mechanisms of intraspecific nucleolar dominance. Both parts of the split NOR proved to be transcriptionally active, and are not subject to intrachromosomal nucleolar dominance. Thus, translocation-induced intraspecific nucleolar dominance is probably the result of interaction of NOR6 and NOR7 or other genetic factors located on the NOR-bearing chromosomes 6(6H) and 7(5H).

Chromosome Inversion↗
PubMed2001

[Complex effects of cavinton on climacteric symptoms].

The use of nonhormonal drugs in order to ameliorate climacteric symptoms makes it possible to treat also those women in whom there are some contraindications or lack of compliance for taking hormonal replacement therapy. The drugs with complex effects on the whole body functions are more widely used recently. The aim of the present study is to assess the therapeutic effect of Cavinton (vinpocetin) on the degree of climacteric symptoms and to verify its complex beneficial influence. The study comprises of three groups of women in early menopause--control group (n = 30), treatment groups with normolipidaemia (n = 32) and with hyperlipidaemia (n = 29). All women presented with moderately expressed climacteric symptoms as assessed by Kupperman menopausal index and Hamilton-Anxiety-Skala (HAMA). The women in the 2nd and 3rd groups have been taking Cavinton in an oral dose of 5 mg three times daily for 3 months. The following parameters of lipid metabolism were determined in the beginning and at the end of the study: total, HDL- and LDL-cholesterol, triglycerides, and two indexes of lipid atherogenic risk--total/HDL-cholesterol ratio and atherogenic index (AI) = total--HDL/LDL-cholesterol. The menopausal complaints were assessed by Kupperman index and HAMA. Blood vessels reactivity was determined by pulsation index (PI). Statistically significant decrease in total cholesterol and LDL-cholesterol levels as well as amelioration of atherogenic indexes was observed in the 3rd group. Kupperman index and HAMA decreased significantly on the 45th day and the 3rd month in the women under treatment. No significant changes in PI were observed but a tendency towards a decrease was seen in the 3rd group. Our data suggest that Cavinton possesses complex beneficial effects in climacteric women significantly ameliorating climacteric symptoms as well as some parameters of lipid metabolism in women with hyperlipidaemia.

Administration, Oral↗
PubMed2001

[Possibilities for an individualized approach to the treatment of climacteric symptoms with phytoestrogens].

UNLABELLED: In climacteric women with contraindications and fear or lack of compliance for taking hormonal replacement therapy as well as in those cases where risks exceed benefits, the use of phytoestrogens is appropriate having in mind their especially good effect on neurovegetative symptoms. AIM: The present randomized prospective study is aimed at estimating the possibility for ameliorating climacteric symptoms with Melbrosia and at assessment of its effect on different symptoms in order to individualize the therapeutical approach. PATIENTS AND METHODS: Two groups of women with climacteric complaints were followed-up--control group (n = 32) and treatment group (n = 34). Climateric symptoms were assessed in the beginning and at the end of the study using Kupperman menopausal index. The levels of FSH, LH, oestradiol as well as some parameters of lipid metabolism were determined. RESULTS: A significant decrease of Kupperman index was observed in Melbrosia treatment group in contrast to the control group. No significant changes in gonadotropins, oestradiol and lipid parameters were found in both groups. CONCLUSION: The preparation Melbrosia, consisting of phytoestrogens and amino acids exerts a good therapeutic effect on climacteric symptoms mainly on the nervousness, anxiety, irritability, headache, and hot flashes.

Amino Acids↗
PubMed2001

[Klion D - possibilities of prophylaxis and treatment during pregnancy].

OBJECTIVE: To evaluated and dilated possibilities of prophylaxis and treatment of agent Klioton D in pregnant women. MATERIAL AND METHODS: 164 women were evaluated for 1.5 year period between 12 and 40 gest. week. The first group of prophylactic usage was consisted of 118 women, divided in two groups: prophylaxis before cerclage--33 women, between 12 and 20 gest. week, and prophylaxis before delivery--85 cases. The second group was therapeutic usage in 46 women between 16 and 34 gest. week. RESULTS: Microbiologically superiority of unspecific bacterial agents was proved, mixed bacterial aerobic-anaerobic flora was dominated. More common bacterial vaginosis and candidosis were noted. In therapeutic group 80% were cured. The rate of subjective complaints in two groups was evaluated. Complications after delivery were 0.04%. Complications after cerclage weren't noted. CONCLUSION: Microbiological type find in pregnant women and good prophylactic and the therapeutic results make Klion D as appropriate therapeutic agent before cerclage, delivery, in imminent abortions and premature labors.

Administration, Intravaginal↗
PubMed1999

Sexual dimorphism in white campion: complex control of carpel number is revealed by y chromosome deletions.

Sexual dimorphism in the dioecious plant white campion (Silene latifolia = Melandrium album) is under the control of two main regions on the Y chromosome. One such region, encoding the gynoecium-suppressing function (GSF), is responsible for the arrest of carpel initiation in male flowers. To generate chromosomal deletions, we used pollen irradiation in male plants to produce hermaphroditic mutants (bsx mutants) in which carpel development was restored. The mutants resulted from alterations in at least two GSF chromosomal regions, one autosomal and one located on the distal half of the (p)-arm of the Y chromosome. The two mutations affected carpel development independently, each mutation showing incomplete penetrance and variegation, albeit at significantly different levels. During successive meiotic generations, a progressive increase in penetrance and a reduction in variegation levels were observed and quantified at the level of the Y-linked GSF (GSF-Y). Possible mechanisms are proposed to explain the behavior of the bsx mutations: epigenetic regulation or/and second-site mutation of modifier genes. In addition, studies on the inheritance of the hermaphroditic trait showed that, unlike wild-type Y chromosomes, deleted Y chromosomes can be transmitted through both the male and the female lines. Altogether, these findings bring experimental support, on the one hand, to the existence on the Y chromosome of genic meiotic drive function(s) and, on the other hand, to models that consider that dioecy evolved through multiple mutation events. As such, the GSF is actually a system containing more than one locus and whose primary component is located on the Y chromosome.

Chromosome Deletion↗
PubMed1997

[Diagnostic laparoscopy in female sterility].

Over a three-year period (1990-1993), a total of 410 diagnostic laparoscopies are performed. The series includes female patients with a minimum one year longstanding of sterility. Diagnostic laparoscopy is taken to be a routine method of making exact diagnosis in female sterility cases, and as a filtering procedure in forthcoming reconstructive operation or in the event of poor outlooks of eventual conception. The commonest finding is endometriosis (in 58.2 per cent), next ranking adhesions free of endometriosis (31.4 per cent) and bilateral obstruction of the uterine tubes (38.5 per cent). Endometriosis is usually located in the uterine body 27.5 per cent, uterine tubes 26.1 per cent and pelvic peritoneum 14.9 per cent.

Adult↗
PubMed1995

[A retrospective comparison between clinical cases and graphs of biorhythms].

The present article develops the hypothesis of the biorhythms in a retrospective study of 50 normal parturitions, 13 premature births of dead fetuses, 9 premature rip off of the placenta, 4 caesarean sections with spinal anaesthesia and unmotivated high arterial pressure and 2 cases of clinical death, one of which treated successfully to life again. The results show high mathematical probability, i.e. 53% of all the incidents had happened exactly in the most critical days. The authors consider correctly that a number of preliminary planed surgical interventions could be in conformity not only with the determined by the biorhythms days but with the most convenient for the patient hour, too. And this is a routine already in a lot of western countries, and especially in the fields of oncological surgery.

Cesarean Section↗
PubMed1993

[The use of midazolam and flumazenil for short-term anesthesia in terminating an early pregnancy].

Aim of the study is to assess the efficacy of flumazenil when used for recovery after midazolam-induced sedation in short acting, out-patient procedure like interruption of early pregnancy. A significantly faster recovery of the patients after injection of flumazenil was observed. When flumazenil was not used, patients remained drowsy, worse oriented, and less co-operative at 1.5 and 20 min after completion of abortion. There were no significant difference between the groups with and without flumazenil at the 60 min. In conclusion when it is possible to observe the patients for more than one hour, the application of flumazenil after sedation with midazolam is not necessary.

Abortion, Induced↗
PubMed1991

[A clinical trial of the preparation hypnomidate in gynecological operations].

The influence of the venous anaesthetic hypnomidate (Yansen, Belgium), used as an induction anaesthetic, was studied on some parameters of respiration, hemodynamics, acid-base balance. The authors are of the opinion that the preparation is suitable for induction in anaesthesia especially in patients with high risk.

Anesthesia, Intravenous↗
PubMed1989

[Epidemiologic studies of the prevalence of arterial hypertension among industrial workers in Stara Zagora].

An epidemiologic study was carried out in June 1986 among 1702 industrial workers from the machine-building and metal-manufacturing industry--956 men and 746 women. 57.9% of the workers were in the 30-39 and 40-49 age groups. Arterial hypertension was found in 16.6% of the examined and borderline hypertension was found in 12.9%. With advancing age the hypertension frequency increases. Men suffer more frequently (18.4%) than women (14.2%). In younger age this difference is statistically significant. A relation was established between hypertension and obesity in both sexes. The characteristics of the hypertension structure and the relation of borderline hypertension with age, sex and body mass are discussed.

Adult↗