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Biomedical subjects

R Voss

Publications and source records attributed to R Voss.

At least 91 records · Page 5Linked to original sources
PubMed1982

Active genes are sensitive to deoxyribonuclease I during metaphase.

The active exogenous murine leukemia virus sequences of mouse cells growing in culture are preferentially digested by deoxyribonuclease I in metaphase chromosomes. As determined by nuclear nick translation, all of the gene sequences of these cells active during interphase are in a deoxyribonuclease I-sensitive conformation during metaphase. This method of nick translation can therefore be used to label chromosomes in situ in order to visualize the active regions of the genome.

Animals↗
PubMed1982

Partial trisomy 15 in a male with severe psychomotor retardation (48, XY, + 15q -, + mar(15)).

An abnormal karyotype consisting of 48 chromosomes has been found in a 16-year-old boy with severe psychomotor retardation and congenital anomalies. The two extra chromosomes: a 15q - and a small metacentric marker appear as derivatives of chromosome 15(48, XY, + 15q -, + mar(15). The healthy father has a mosaic chromosome constitution 46, XY/47, XY, + mar(15) and this additional chromosome is also present in the propositus. It is suggested that the presence of the extra chromosome in the father's germ cells may play a role in the production of the additional 15q - chromosome responsible for the clinical syndrome of the propositus.

Abnormalities, Multiple↗
PubMed1982

Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia.

The GDM-1 permanent cell line was established from the peripheral blood of a patient with a Philadelphia chromosome negative myeloproliferative disorder, after transformation to acute myelomonoblastic leukemia. The GDM-1 cells exhibited the same characteristics as those isolated from the peripheral blood of the patient prior to death: cells contained non-specific esterase sensitive to fluoride, myeloperoxidase, lysozyme (muramidase), and exhibited both Fc and complement (C3) receptors but lacked B- and T-cell surface markers including T-associated antigens. E-rosetting capacity, surface and intracytoplasmic immunoglobulins and EBV determined nuclear antigen (EBNA). The GDM-1 cells bore the 1a receptor and the myeloid leukemia antigen (M-1). The karyotype of the cultured leukemic cells showed the same specific chromosomal abnormalities present in the monoblasts obtained from the peripheral blood prior to death, indicating that the cell line was derived from the original leukemic cells.

Aged↗
PubMed1982

Prostacyclin production of the isolated pulsatingly perfused rat aorta.

The abdominal aorta of the rat was isolated and perfused. Pulsations were found to be a prerequisite for prostacyclin production in the isolated aorta. In the pulsatingly perfused aorta, the prostacyclin production decreases rapidly during the first 30 min of perfusion and very slowly between 30 and 120 min. The technique offers a good means of investigating the effects of different agents on prostacyclin production.

Adenosine Diphosphate↗
PubMed1982

Growth of the dental arches after ablation of the mid-palatal suture. A study in the domestic cat.

The present investigation comprises a group of 18 domestic cats with surgically created, and finally histologically verified, submucous mid-palatal clefts. In addition 18 unoperated control animals were examined, along with 12 sham-operated cats, the latter serving as controls on the effect of the surgical soft tissue trauma applied. Measurements were made in regard to width and length of the dental arches, starting at the age of 2 1/2 months, ending at 12 2/3 months, i.e. throughout both the deciduous and the permanent dentition. Data of existing dimensions and growth were obtained. The statistical analysis revealed a significant reduction in final transverse dimensions in the posterior regions of both the upper and the lower dental arches in the deciduous and permanent dentition, in animals where the mid-palatal suture was missing. The soft tissue trauma did not seem to interfere significantly with normal growth.

Animals↗
PubMed1982

Compensatory growth of the dental arches in hypoplasia of the maxillary base. A biometrical analysis in the domestic cat.

When two opposing layers of mucoperiosteum are used to cover experimental mid-palatal clefts in growing cats, bone bridges regularly form between the palatal halves. This results in significantly smaller transverse dimensions of the hard palate, in a hypoplastic maxillary base. The present investigation analysed the concurrent enlargement of the dental arches in such animals. Compared with un- and sham-operated controls, no significant divergencies could be found. The cat has a deep scissor bite. A reasonable interpretation of the findings might therefore be that mandibular dental arch growth is transmitted 'orthodontically' to the maxillary teeth, through the occlusion. In cleft lip and palate patients operated on with conventional methods, similar bone bridges are frequently found, but also a favourable effect of orthodontic treatment on co-existing dental malocclusions. To some extent the findings of the present study appear to coincide with those of the clinic. The results seem to indicate that adjustment of dental arch dimensions in disproportionate jaw bases may be due more to conductible compensatory dental arch growth than to forcibly induced movements of jaw segments.

Age Factors↗
PubMed1982

Bony or connective tissue union of operated experimental palatal clefts: implications on growth of the dentomaxillary complex. A biometrical study in the domestic cat.

Operated human cleft palates heal with formation of bone and/or connective tissue in the mended defect (Prydsö, Holm, Dahl & Fogh-Andersen, 1974; Enemark & Jörgensen, 1978). In domestic cats with experimental palatal clefts, reparation by hard or soft tissue appears to be conditioned by the type of palatoplasty applied (Freng, 1979a; 1979b;). Both tissue responses led to reduced transversal growth of the maxillary base (Freng, 1979c; 1981). As regards the concurrent dental arch growth, however, no difference from unoperated controls was found in individuals with bone postoperatively bridging the palatal mid-line (Freng & Voss, 1982). A submucous cleft led to a significant reduction in transversally directed arch development (Voss & Freng, 1982). The present study aimed to find out if these observations could be due to a relatively smaller width of the maxillary base in operated cleft palates healing with connective tissue. However, no significant difference in maxillary base width appeared when the two groups were compared. To some extent, the effect of the cat's scissor bite, on the dental occlusal relationship, may simulate the effect of orthodontic appliances in humans by transmitting mandibulary growth into laterally directed movement of the upper jaw's teeth. The difference in dental arch growth between the two groups might therefore be interpreted either as expressing a better preservation of obtained compensatory transversal maxillary teeth movements in palates healing with bone bridges, or a tendency for the upper dental arch to collapse in cases with submucous mid-palatal clefts.

Animals↗
PubMed1982

Influence of dietary linoleic acid on the prostacyclin-production of the isolated pulsatingly perfused rabbit aorta.

Dietary prevention of atherosclerosis and its risk factors by linoleic acid is an easy, safe and important way. In the rabbit, which develops an atherosclerosis similar to the human disease, we looked for effects of a linoleic acid-rich diet (25 en percent) on the formation of arachidonic acid metabolites in the vessel wall and in blood platelets because atherosclerosis is thought to be caused by a change in this balance. For measuring the PGI2-release of the vessel wall we used the model of the isolated pulsatingly perfused aorta, which is described in detail. Both dietary groups of healthy male Dutch rabbits did not show any difference in the formation of arachidonic acid metabolites or in their balance. With 25 en percent linoleic acid the phospholipids contained more linoleic acid and less oleic acid, while arachidonic acid tended to decrease.

Animals↗
PubMed1981

Prenatal diagnosis of Fanconi anemia.

Prenatal diagnosis was performed on a fetus at risk for Fanconi anemia. A high spontaneous (0.30 breaks/cell) and diepoxybutane-induced (0.69 breaks/cell) chromosome breakage rate indicated an affected fetus and the pregnancy was terminated. The anatomic findings in the aborted fetus together with cytogenetic findings in cultured fetal skin fibroblasts confirmed the prenatal diagnosis.

Abnormalities, Multiple↗
PubMed1981

Ultrastructural, cell membrane, and cytogenetic characteristics of B-cell leukemia, a murine model of chronic lymphocytic leukemia.

A murine model of a spontaneous, transplantable BALB/c B-cell leukemia (BCL1) is described. Extreme leukemia and splenomegaly develop in H-2d-compatible recipients of tumor cells. Tumor cells are medium to large lymphocytes that can be transformed into plasmacytoid cells following in vitro stimulation with lipopolysaccharide. Karyotypic analysis of transformed tumor cells reveals 36 chromosomes with several monosomies and 7 markers chromosomes. The ultrastructure of the tumor cells was studied using transmission and scanning electron microscopy. Although the appearance of tumor cells seems normal by morphological criteria, an impaired capping ability was documented using the fluorescein-conjugated concanavalin A-binding test. Impaired capping ability was documented before leukemia was overt as early as 1 to 3 days following inoculation of tumor cells. The B-cell leukemia (BCL1) provides a useful murine model for the study of various aspects of human bone marrow-derived malignant disorders.

Animals↗