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Biomedical subjects

R J Jones

Publications and source records attributed to R J Jones.

At least 199 records · Page 11Linked to original sources
PubMed1979

Controlled trials of a polyvalent pseudomonas vaccine in burns.

A polyvalent pseudomonas vaccine has been tested in controlled clinical trials at two burns units, in Birmingham and New Delhi, in children and adults with burns more than 15% full skin thickness. None of the vaccinated patients in either trial showed blood cultures containing Pseudomonas aeruginosa, and vaccinees showed raised titres of protective antibody and increased phagocytic activity against Ps. aeruginosa. In the New Delhi unit, where death from Ps. aeruginosa infection is common, the mortality in adults was reduced from 40.6% (13/32) in the unvaccinated group to 6.6% (2/30) in the vaccinated group, and in children from 20.8% (5/24) in the unvaccinated group to 4.8% (1/21) in the vaccinated group.

Adolescent↗
PubMed1979

Measurement of endotoxins with the limulus test in burned patients.

High titres of endotoxin as measured by the Limulus test were usually found in burned patients who had raised body temperatures, and were colonized with gram-negative bacteria; also some infected patients showed raised endotoxin without a raised temperature. Patients vaccinated with an antipseudomonas vaccine rarely showed endotoxin in their plasma but occasional plasma samples from vaccinated patients had a high titre of endotoxin which appeared unrelated to infection or to a raised temperature in the patient.

Adolescent↗
PubMed1979

A randomized study of instructional variations in nutrition counseling and their efficacy in the treatment of hyperlipidemia.

The important of the educational diagnosis in instructing patients in a fat modified diet was examined in a double blind study. The educational diagnosis depended upon the assessment of three independent instructional parameters: the amount of structure, the teaching mode, and the language code preferred by the patient. Group I (67 patients) received traditional personalized counseling for 3 months. Group IIa (40 patients), whose instruction was matched to their preferred education attributes, was randomized against group IIb (36 patients), whose instruction was mismatched. Group IIIa (33 patients), receving matched instruction was randomized against group IIIb (35 patients), receiving high structure in the symbolic mode of instruction. The greatest reduction in serum cholesterol was seen in group I. At 3 months, no significant difference in serum cholesterol reduction could be shown between groups IIa and IIb, or IIIa and IIIb. However, the mean cholesterol reduction became significantly greater (P less than 0.05) in IIa than IIb at the 6 month visit. Seven day food diaries showed no significant differences in dietary intake and 1 and 3 months between matched and unmatched groups. The data did suggest that patients preferring high structure in the symbolic mode had a greater serum cholesterol reduction that other diagnostic groups. Analysis of variance showed an interaction between the preferred and instructional level of structure as related to the change in total fat, and saturated fat of the diet after 3 months.

Audiovisual Aids↗
PubMed1979

The NBT test in burned patients.

The number of polymorphs which stained with the dye nitro-blue tetrazolium (NBT "Positive") increased sharply during the first week after burning, reaching levels 4--5 times above values for healthy volunteers. In burns of more than 20% of the body surface a second, smaller increase in the number of NBT "positives" occurred 4 to 6 weeks after burning. The high levels of NBT "positive" polymorphs occurred independently of infection on the burns. A burned patient who died from septicaemia had very low numbers of NBT "positive" polymorphs for 3 weeks before death.

Adolescent↗
PubMed1978

Toxicity of Leucaena leucocephala. The effect of iodine and mineral supplements on penned steers fed a sole diet of Leucaena.

Steers fed a sole diet of Leucaena leucocephala cv. Peru (leucaena), rapidly developed a hypothyroid condition. Total serum thyroxine (T4) levels declined from c 120 n mol litre-1 to 13 n mol litre-1 within six weeks of full leucaena feeding. Associated with the hypothyroidism was a declined in feed intake, poor weight gain, hair loss, excessive salivation and the development of lesions on the oesophagus of some animals. Light steers (119 kg) were more severely affected than heavy steers (220 kg) and two light steers died. During the eight week period of full leucaena feeding animals gained only 0.18 kg head-1 day-1 compared with 0.73 kg head-1 day -1 for steers fed cowpea (Vigna sinensis) hay. Supplementation with minerals (Fe, Cu, Zn) significantly increased mean daily intake (85.4 v 68.9 g/kg0.75), daily liveweight gain, (334 v 14 g), and serum T3 resin uptake (42.5 v 39.6%); decreased hair loss and skin lesions but did not alleviate the low serum T4 levels. Iodine supplementation did not affect intake, bodyweight gain, serum T4 levels, serum T3 uptake or toxicity scores compared with unsupplemented leucaena fed steers. When the leucaena fed animals were subsequently fed cowpea hay, appetite improved and the serum T4 levels returned to normal within two weeks. For the heavy steers over this period, weight gain was similar to the controls; however, the light steers continued to gain poorly.

Animals↗
PubMed1977

A new polyvalent Pseudomonas vaccine.

This paper describes the preparation, chemical characterisation and immunogenic properties of a new polyvalent pseudomonas vaccine. New cultural methods were devised which allowed a build-up of the immunogen in the cell wall of the bacteria, and mild extraction techniques were used to remove the immunogens from the cell wall of living bacteria without apparent physical or chemical change which might affect immunogenicity. The polyvalent vaccine comprised 16 component vaccines, each a lipid-protein-carbohydrate complex extracted from one of the 16 different serotypes of Pseudomonas aeruginosa. Single injections into mice of each of the 16 component vaccines induced protection against several strains of homologous serotypes within 3 days of vaccination. The polyvalent vaccine induced similar protection against several strains of each of the 16 serotypes. We would like to thank R. E. Dyster, K. Digby and J. Simmonds for their technical assistance.

Animals↗
PubMed1976

A new Pseudomonas vaccine: preliminary trial on human volunteers.

Fifteen healthy volunteers were given three weekly subcutaneous injections of a new polyvalent Pseudomonas aeruginosa vaccine (PEV-01). Four doses - 1-0 RHD (manufacturer's recommended human dose), 0-75 RHD, 0-5 RHD and 0-1 RHD - were used in separate groups of volunteers. Blood samples taken before each of the injections and one taken 7 days after the last injection were examined for immune response to the vaccine and for possible adverse clinical, biochemical and haematological effects. Raised titres of antibody in serum of volunteers given 0-5-1-0 RHD vaccine were shown, often by the seventh day, in passive haemagglutination tests against all of the 16 serotypes of Ps. aeruginosa represented in the vaccine; serum from volunteers who received 0-1 RHD usually showed a reduced antibody titre. Tests of mouse protection by serum against intraperitoneal challenge with Ps. aeruginosa P14 showed increased titres of mouse protective antibody in the blood of volunteers given 1-0, 0-75 or 0-5 RHD of vaccine but a reduced mouse-protective titre in two out of three sera from volunteers given 0-1 RHD vaccine. There was a suggestion of enhanced phagocytic ingestion and intracellular killing of two strains of Ps. aeruginosa by the blood of vaccinated volunteers, and more definite enhancement of ingestion of inert latex particles, which were less well ingested than were the bacterial cells by phagocytes from unvaccinated volunteers. Apart from slight or moderate local reactions and a transient rise of temperature in some volunteers, there were no clinical, biochemical or haematological abnormalities in the vaccinated volunteers.

Adolescent↗
PubMed1976

Oral vaccination against Porteus mirabilis.

Mice given a single dose of proteus vaccine orally were protected against 1 MLD (minimum lethal dose) of autologous Proteus mirabilis by the fourth day after vaccination. Three doses of oral vaccine induced protection against 1 MLD autologous challenge for 7 days after vaccination and partial protection for a further 8 weeks. Cross-protection against different strains of Pr. mirabilis and against some strains of Pseudomonas aeruginosa, Serratia marcescens and Providencia species was found in mice immunized orally on three consecutive days with a bivalent proteus vaccine.

Administration, Oral↗
PubMed1975

Esophageal injury.

This review considers all possible modes of esophageal injury, based on a schema originally published in 1954 and more recently modified in 1970. For each category of injury there are detailed discussions of diagnosis and treatment. The best available knowledge of present-day modalities has been based on a survey of the literature for the past decade. When diverse methods of treatment were encountered, we have made comments consistent with our personal experience, when appropriate. The medicolegal literature contains a number of references to esophaegeal injury. Certain excerpts dealing with several types of potential actions are extracted: (1) suits to recover damages for esophageal perforation in which negligent endoscopy was claimed; (2) suits following endoscopic accidents in which lack of informed consent was claimed; and (3) suits for professional liability based on misdiagnosis, delayed diagnosis, or wrong treatment of esophaegeal perforation.

Alcohol Drinking↗