Biogenic amine metabolites in cerebrospinal fluid of patients with affective disorders.
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Adult↗
Biomedical subjects
P Vestergaard
Publications and source records attributed to P Vestergaard.
Comparison of the use of inorganic and organic bases in the Zimmermann reaction for 17-oxosteroids.
A comparison has been made between inorganic and organic bases proposed for use in the Zimmermann reaction for 17-oxosteroids. The use of inorganic base in pyridine solution is preferred for large scale liquid chromatography because of its high sensitivity and its better wetting characteristics. Several of the organic bases tested have desirable characteristics when used for total group assays and may be preferred by some for such assays.
17-Ketosteroids↗
The specificity of multi-column liquid chromatography of neutral urinary steroids as evaluated by comparison with parallel gas chromatography and mass spectrometry.
The use of gas chromatography/mass spectrometry is advocated as the best currently available methodology for the validation of a routine assay for steroid analysis. This is documented by comparative studies by gas chromatography/mass spectrometry and spectrophotometry of peak material obtained in routine assays by multi-column liquid chromatographic techniques.
Chromatography, Gas↗
Quality control measures in the assay of common urinary steroids as applied to a multi-column chromatographic procedure.
Quality control measures in the assay of common urinary steroids include stringent controls not only with details of the procedure but also, through a steady monitoring of the output, with the use of "blind" duplicates, the application of estimates of duplicate variability, comparison of the sum of individual steroids chromatographically estimated with independent total group assays and the use of internal standards in the procedure.
Chromatography, Liquid↗
Excretion of common neutral steroids in healthy subjects as estimated by multi-column chromatography.
Excretion data for common neutral urinary steroids from a total of 330 healthy subjects from different parts of the world and of different sex and age are given. The estimations, which have been performed by multi-column liquid chromatography, include 24 h excretion values for both common 17-oxosteroids and the common metabolites of cortisol, including the cortolones and the cortols. Comparisons are made with values from the world literature and with isotope experiments.
17-Ketosteroids↗
Comparison between total group assays for urinary 17-oxosteroids and 17-oxogenic steroids and estimates of individual urinary steroids.
A comparison between a group assay for total 17-oxosteroids in urine and the summed values for individual 17-oxosteroids shows a high correlation between these sets of values. Similarly, a group assay for 17-oxogenic steroids was found to correlate highly with the sum of individual corticosteroids.
17-Ketosteroids↗
Multi-column chromatography and the use of isotopes in the study of steroid metabolism.
Multi-column liquid chromatography is demonstrated to be a technique well suited for isotope experiments involving administration of labelled cortisol. It has potential for secretion rate determinations, for dynamic studies of cortisol metabolism and for work with stable isotopes.
Chromatography, Liquid↗
Multi-column chromatography of neutral urinary steroids and metyrapone testing.
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Chromatography, Gas↗
Multi-column chromatography of urinary steriods and adrenocortical dysfunction.
The potential of the multi-column assay for urinary neutral steroids in work with samples from patients with adrenocortical pathology is demonstrated through analyses performed on urine samples from Cushing and congenital adrenal hyperplasia cases, after modification of the routine methodology to include the quantitation of additional steroids of particular importance for pathological samples.
Adrenocortical Hyperfunction↗
Estimation of 17-oxosteroids in plasma by multi-column chromatography.
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17-Ketosteroids↗
Multi-column chromatography of urinary 17-ox osteroids as their 2,4-dinitrophenylhdrazones.
The use of 2,4-dinitrophenylhydrazones in the multi-column chromatography of 17-oxosteroids from urinary extracts has been possible on mixed columns of alumina and silica gel. Preliminary results indicate agreement with values obtained using the Zimmermann reaction. The hydrazones have the advantage of color stability, when compared with the Zimmermann reaction, and the analysis is simpler and cheaper to perform.
17-Ketosteroids↗
The multicuvette general analytical system for combined multi-column chromatography and high capacity single substance and group analyses and its application to steroid analyses.
A description is given of the development of a general analytical system for both single and group analyses and for analyses of profiles of compounds after multi-column liquid/liquid and liquid/solid chromatography. Common for both branches of the system is a computerized quantitation system built around a multi-compartmented cuvette. The system is compared with other high capacity systems for biochemical analyses. Special attention is given to the application of the system to the analysis of urinary steroids, both individual and as groups, with a comparison with similar methology using other techniques.
Autoanalysis↗
General methodology for the estimation of common neutral urinary steroids by multi-columin liquid chromatography.
A method is described for the estimation of common neutral urinary steroids by multi-column liquid chromatography. Hydrolysis is performed in two steps: enzymatically, using beta-glucuronidase, followed by solvolysis. Initial short column chromatography separates the neutral steroids into three fractions according to polarity: 17-oxosteroids, corticosteriods less polar than cortolones, and cortolones and cortols. The cortolone, cortol fraction is oxidized and the different steroid groups are chromatographed on capillary aluminum oxide and silica gel columns. A computerized, spectrophotometric system is used for the quantitation procedure.
17-Ketosteroids↗
The hydrolysis of conjugated neutral steroids in urine.
Complete hydrolysis of known conjugates of neutral steroids in urine requires a combination of solvolysis and enzymatic hydrolysis. Procedures are established for optimal yield in the enzymatic part of the hydrolysis using different preparations of beta-glucuronidase. The effect of temperature and the use of conjugate extraction in the hydrolytic procedure is explored. It is stressed that relatively high concentrations of enzyme are necessary to insure full hydrolysis of a few conjugates that are particularly difficult to split by enzymatic hydrolysis.
Animals↗
Factors affecting the excretion of common neutral steroids in urine.
After a brief summary of the biosynthesis, production rates, metabolism and elimination of the pertinent adrenocortical and gonadal precursors for the common urinary neutral steroids, a survey is presented of the general, disease related and hormonal factors that can affect the excretion of neutral steroids in urine.
Adrenal Cortex Hormones↗
Relationship between steroid excretion patterns and breast cancer incidence in Israeli women of various origins.
A nationwide study of the steroid excretion patterns in postmenopausal Israeli migrant women demonstrated differences between high- and low-risk groups for breast cancer in the following variables: age at first parturition, number of pregnancies, number of live births, height, and weight. The direction of the differnces was in line with those observed for breast cancer patients. The groups also differed in the exretion of estriol, 17-ketosteroids, and allotetrahydrocortisol. Multiple regression analysis revealed that the exretion of estriol was significantly lower in population groups in whom breast cancer incidence was high. Possibly this trend--which has also been observed in adolescent and premenopausal women--reflected environmental influences on peripheral estrogen metabolism.
17-Ketosteroids↗
Total and subtotal colonoscopy with short instrument and without fluoroscopy.
The technique and results of 111 consecutive colonoscopies with a short colonoscope without the use of X-ray screening are reviewed. The caecum could be reached in 44 percent of the cases. No complications occurred. The great clinical value of the procedure is shown by the following findings:X-ray-negative lesions--including 2 cases of carcinoma--were found in 35 percent of the cases, radiologically demonstrated lesions could be defined more precisely in 18 percent, and the presence of colonic lesions could be ruled out in 11 percent in spite of equivocal X-ray findings. It is concluded that many more centres ought to take up the procedure, which offers the prospect of significantly reducing suffering and death from colonic disease.
Adult↗
Lithium treatment of Huntington's chorea. A placebo-controlled clinical trial.
The therapeutic effect of lithium in Huntington's chorea was tested in six patients through a double-blind cross-over comparison of lithium and placebo, each administered for 6 weeks. Four of the patients received neuroleptic drugs at the same time. Lithium and placebo periods did not differ as regards motor skills, hyperkinesias, or total ward situation, all rated quantitatively with the use of scales. Lithium does not seem to be of therapeutic value in Huntington's chorea.
Adult↗