Search PubMed⌕ Search

Biomedical subjects

N Komatsu

Publications and source records attributed to N Komatsu.

At least 217 records · Page 12Linked to original sources
PubMed1984

A stability study on Gc subtyping in bloodstains: comparison by two different techniques.

The limits of determination of Gc subtypes in bloodstains were compared between the immunofixation method and the sulfosalicylic acid precipitation method using isoelectric focusing on polyacrylamide gel. By the immunofixation method Gc subtyping in bloodstains was successfully made at 37 degrees C after 7 weeks, at room temperature after 17 weeks and at 4 degrees C even after 25 weeks storage. By the sulfosalicylic method Gc subtypes were no longer able to be determined a few weeks after stain formation. The superiority of the results obtained by the immunofixation method makes it the recommended method for the Gc subtyping from bloodstains in medicolegal practice.

Benzenesulfonates↗
PubMed1983

Mesangial deposition of J chain-linked polymeric IgA in IgA nephropathy.

Renal biopsy specimens from 8 patients with IgA nephropathy (Berger's disease) were examined immunocytochemically at the light and electron microscopic levels with peroxidase-labeled antibodies to IgA, IgM, J chain and secretory component. In 2 of the 8 specimens heavy deposits of IgA, but no IgM, were found in the mesangium. After acid-urea treatment of these tissue sections, J chain, a subunit of polymeric immunoglobulins, was identified in a distribution similar to that of IgA. In the remaining 6 specimens, small amounts of IgM in addition to denser deposits of IgA and J chains were found. We conclude that the IgA deposits in at least some patients with Berger's disease consist of IgA polymers linked by J chain.

Animals↗
PubMed1982

Regulation of cytochrome P450 activities in adrenocortical mitochondria from normal rats and human neoplastic tissues.

From our immunocytochemical studies, cytochrome P450scc and P45011 beta systems were localized on the matrix side of inner membrane of the mitochondria in the parenchymal cells of adrenal cortex. However, the degree of immunocytochemical staining varied from one mitochondrion to another within a single cell; some stained intensely along the entire inner membrane, some stained only along segments of the inner membrane, and some did not stain at all. The ratio of stained to unstained mitochondria was approximately unity in untreated rats, while stained mitochondria greatly increased upon ACTH administration suggesting that the population of cytochrome P450 system-containing mitochondria increased upon long-term ACTH action. By the combined use of flash photolysis and substrate difference spectroscopy, quantitative determination of substrate-bound and free forms of P450scc and P45011 beta in mitochondria became possible. Increases in total amounts of P450scc and cholesterol resulted from the long-term ACTH administration to rats. The ratio of cholesterol-bound P450scc to its free form was essentially unchanged under these conditions. On the other hand, amounts of P45011 beta were not increased significantly by long-term ACTH administration. The ratio of DOC-bound P45011 beta to the free form increased significantly, however, by long-term ACTH administration and also in the mitochondria from patients suffering from Cushing's syndrome.

Adrenal Cortex↗
PubMed1982

Amorphous and stellate amyloid in functioning human pituitary adenomas: histochemical, immunohistochemical and electron microscopic studies.

Two types of amyloid, amorphous and stellate, were studied in 3 functioning human pituitary adenomas (2 prolactin-secreting and one growth hormone (GH) secreting) by histochemical and immunohistochemical techniques and by electron microscopy. The amorphous amyloid was found in all 3 adenomas, but the stellate type was observed only in the Gh-secreting tumor. Both types of deposits satisfied the staining characteristics of amyloid and were composed of aggregated filaments, 30-300 A in diameter by electron microscopy. The stellate type was strongly positive for GH by immunohistochemistry and showed ultrastructural transition to surrounding tumor cells, findings strongly suggestive of a hormonal origin. The presence of scattered secretory granules in the amorphous type was correlated with weak immunostaining for prolactin in the amyloid.

Adenoma↗
PubMed1982

Cytochrome P-45011 beta and P-450scc in adrenal cortex: zonal distribution and intramitochondrial localization by the horseradish peroxidase-labeled antibody method.

In an attempt to elucidate the regulation mechanism(s) of adrenocortical steroidogenesis, cytochrome P-450scc and cytochrome P-45011 beta were localized in bovine adrenal glands by the direct peroxidase-labeled antibody method. At the light microscopic level, parenchymal cells of the zona fasciculata and the zona reticularis stained heavily for both cytochromes, while the parenchymal cells of zona glomerulosa stained lightly for both. At the electron microscopic level, these two cytochromes were associated with the matrix side of the inner mitochondrial membranes of parenchymal cells from all three zones of the adrenal cortex. The association of cytochrome P-450 with the inner mitochondrial membrane, in a manner similar to that previously reported for adrenodoxin and adrenodoxin reductase (F Mitani, Y Ishimura, S Izumi, K Watanabe, Acta Endocrinol 90:317, 1979), establishes that the steroid monooxygenase systems exist at this site. The degree of immunocytochemical staining within a single cell varied from one mitochondrion to another: some stained intensely along the entire inner membrane, including the cristae, some stained only along segments of the inner membrane, and some did not stain at all. This heterogeneity in staining was observed in mitochondria stained in situ as well as in isolated mitochondria. These findings suggest that there is a heterogeneity in steroidogenesis among mitochondria contained within a single cell of the adrenal cortex.

Adrenal Cortex↗
PubMed1982

Ultrastructural localization of prolactin in the rat anterior pituitary glands by preembedding peroxidase-labeled antibody method: observations in normal, castrated, or estrogen-stimulated specimen.

Ultrastructural localization of prolactin (PRL) was studied immunocytochemically (preembedding peroxidase-labeled antibody method) in a variety of pituitaries, including those from 1) normal, 2) castrated, and 3) castrated and estrogen-stimulated rats. In the normal rat, PRL was observed in cisternae of the rough endoplasmic reticulum (RER), perinuclear spaces, Golgi saccules, and secretory granules. In the castrated rats, PRL cells were rather atrophic and were filled with many small PRL-positive secretory granules. RER and Golgi saccules were rather inconspicuous and were almost devoid of PRL localization. The serum PRL level was markedly lowered. With estrogen stimulation after castration, the serum PRL level was markedly elevated and PRL cells showed a pronounced increase of PRL filled cisternae in the RER. From these observations, the role of secretory granules, Golgi apparatus, and RER in hormonal secretion was defined, and it was postulated that some peptide hormones would be secreted along two alternative pathways, i.e., either 1) a long (regulated) pathway or 2) a short (accelerated) pathway, in accordance with their secretory activities, which could be altered by various stimulations such as the use of estrogen.

Animals↗
PubMed1981

An epidemic of swine influenza in Japan.

In a serological survey of pigs in Japan haemagglutination inhibition (HI) antibody to swine influenza virus (Hsw1N1) was first detected in 721 of 3313 (21.1 per cent) sera in 1977 and 1578 of 4946 (32.1 per cent) in 1978. The virus was prevalent in all districts of Japan but to a different extent. In contrast to the high incidence of antibody, there were few cases of infection with clinical signs and only 13 outbreaks of swine influenza were recognised in Japan from 1978 to 1979. Thirty-seven influenza viruses were isolated from nasal swabs of diseased pigs in 13 outbreaks. The subtype of most isolates was Hsw1N1 but two strains were Hsw1N2.

Animals↗
PubMed1981

Nasal allergy in spinning mill workers and the possibility of allergenicity of chemical fibers and cotton linters.

During a three-year period from 1976, we examined fifty eight patients with nasal allergy who worked in spinning mills or handled sewing machines. Skin tests and procative nasal tests were performed on these patients with crude and fractionated extracts from cotton linters, cotton cloth and chemical fibers. The crude aqueous antigens were extracted in dextro-phenol solution and fractionated by Saphadex G-100. It was possible that the cotton and chemical fibers included components which were the cause of hypersensitivity, but there is still doubts concerning their reaction as irritant substances. Serum specific IgE antibody was found in extracts from chemical fiber. Therefore, spinning mill dust acts as an irritant of the nose add the possibility of antigenicity of the dust for immediate type nasal allergies can not be denied from the present study.

Acrylic Resins↗
PubMed1980

Purification and immunohistochemical localization of rat liver glutathione peroxidase.

Glutathione peroxidase was purified from the rat liver to give a single protein band in polyacrylamide gel electrophoresis. Rabbits were immunized with this purified enzyme, and a highly specific anti-glutathione peroxidase antiserum was obtained. Using this antibody, an immunohistochemical technique (the indirect method of peroxidase-labeled antibody) was applied to study the localization of the enzyme in the liver cells. On immunohistochemical observation, glutathione peroxidase was localized exclusively in the cytoplasm of hepatocytes, and a stronger 'immuno-staining' was exhibited in the peripheries of the hepatic lobules than in the central zone.

Animals↗
PubMed1980

Light and electron microscopical combined staining by immunohistochemical and enzyme histochemical methods using rat prolactin-secreting pituitary tumours.

Combined immunohistochemical staining (IHCS) and enzyme histochemical staining (EHCS) methods for light microscopy (LM) and electron microscopy (EM) are reported, using oestrogen-induced rat pituitary tumours. For LM, combined staining for alkaline phosphatase and acid phosphatase by EHCS, using the azo dye method, and for prolactin and ACTH by IHCS, using the enzyme-labelled antibody method, gave the best results on 1 microgram glycol methacrylate sections. For EM, combined staining by EHCS on 30 microgram tissue sections followed by IHCS for prolactin on ultrathin Epon sections (enzyme-labelled antibody method) provided acceptable results. By these combined staining methods, the neoplastic prolactin cells were shown to have close affinity to rich alkaline phosphatase-positive capillaries and to possess an alkaline phosphatase-positive cell membrane. Furthermore, they revealed acid phosphatase-positive lysosomal and secretory granules. These combined staining methods may be valuable in studies on the actual functional status of cells.

Acid Phosphatase↗
PubMed1980

Streptomyces spadicogriseus, a new species producing anthramycin.

The taxonomic description of Streptomyces spadicogriseus, a new species belonging to the Gray Series of streptomycetes as classified by Pridham and Tresner, is presented. This new species is distinguishable from the known members of the Gray Series. Streptomyces spadicogriseus produces anthramycin but bears no taxonomic relation to the known producer of the antibiotic: S. refuineus var. thermotolerans.

Animals↗