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Biomedical subjects

N Komatsu

Publications and source records attributed to N Komatsu.

At least 199 records · Page 11Linked to original sources
PubMed1985

Polymorphism of EsD by isoelectric focusing: description of the new allele EsD*Kofu and phenotyping in bloodstains.

The polymorphism of EsD was investigated in 1115 unrelated Japanese individuals by isoelectric focusing. Besides the three common phenotypes two heterozygotes EsD 7-1 and EsD 7-2 were observed. The gene frequencies were: EsD*1 = 0.6234, EsD*2 = 0.3663, and EsD*7 = 0.0103. In addition, a rare variant was detected in a probandus living in the city of Kofu. The family analysis suggested the hereditary occurrence of a new allele EsD*Kofu. The isoelectric focusing method was successfully applied to phenotyping EsD in bloodstains; each phenotype was demonstrated at 37 degrees C for up to 2 weeks, at room temperature for up to 9 weeks, and at 4 degrees C for over 20 weeks after stain formation.

Alleles↗
PubMed1985

Immunocytochemical and enzymecytochemical studies on the intracellular transport mechanism of secretory immunoglobulin A and lactoferrin in human salivary glands.

The intracellular transport mechanism of secretory immunoglobulin A (sIgA) has been immunocytochemically defined in human submandibular glands. To examine the properties of the intracytoplasmic vesicles which contain IgA, the enzyme labeled antibody method for SC and IgA and Novikoff's method for acid phosphatase (ACPase) activity were employed on the same sections. The intracytoplasmic vesicles containing IgA and SC in the serous acinar cells were free of ACPase activity and were thus distinguishable from lysosomes. Neither IgA nor SC was localized in the secretory granules. Lactoferrin was localized in the secretory granules and glandular lumen of the serous acinar cells, but not in the cytoplasmic vesicles, which were also free of ACPase activity. These findings suggests that the transport of sIgA was performed by intracytoplasmic vesicles and that lactoferrin is discharged from secretory granules into the lumen and finally makes a "rendezvous" with sIgA in the lumen of acinar cells.

Biological Transport↗
PubMed1985

Placental-soluble aconitase polymorphism in Japanese.

Polymorphism of soluble aconitase was investigated in 152 Japanese placentae. The allelic frequencies were ACONS1 = 0.951 and ACONS2 = 0.049. ACONS2 appears to be rather high among Orientals including Japanese, while ACONS4 seems to be characteristic for Negroids.

Aconitate Hydratase↗
PubMed1985

A further study on the polymorphism of placental alkaline phosphatase in Japanese.

Polymorphism of placental alkaline phosphatase (PL) was investigated in 120 Japanese placentae by means of starch gel electrophoresis and an improved staining method for the isoenzymes using naphthol AS-MX phosphate as substrate instead of beta-naphthyl phosphate. The present method gave more satisfactory results for the detection of the PL bands. Besides five of the six common phenotypes three different uncommon variants were identified. The gene frequencies of PL types were estimated at PL1 = 0.729, PL2 = 0.054, PL3 = 0.200 and PLv = 0.017, which were very similar to those reported in other districts of Japan.

Alkaline Phosphatase↗
PubMed1985

Polymorphism of placental phosphoglucomutase in Japanese.

Polymorphism of PGM1 and PGM3 types was investigated in placental extracts from 127 unrelated Japanese parturients living in Yamanashi Prefecture. The gene frequencies were 0.787 for PGM1(1), 0.213 for PGM1(2); 0.760 for PGM13, 0.240 for PGM3(2), respectively. There was no significant association between the PGM1 and PGM3 systems. The PGM3(2) frequency in the present sample population was higher than those in Kanto District, but lower than those in Kinki District.

Electrophoresis, Starch Gel↗