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Biomedical subjects

M Wagner

Publications and source records attributed to M Wagner.

At least 1,063 records · Page 59Linked to original sources
PubMed1979

[Effect of a 3.6 o/o dextran Ringer acetate solution on moderately hypovolemic patients in intensive care].

The effects of 1000 ml 3.6% dextran Ringer's acetate solution (Mw 61,800; infusion time 60 min) on circulation, blood and plasma volume, blood viscosity and renal function parameters were investigated, the renal elimination of dextran also being measured. During the entire investigation period no significant alterations of cardiocirculatory parameters, blood and plasma volume and rheological properties of blood could be demonstrated, the same holding true for kidney function values. Dextran excretion was about 33% three hours after the end of infusion, reaching 51% of the whole amount given 21 hours later. It has to be assumed that mainly macromolecular dextran components of less efficiency are retained intravascularly. Taking into consideration the practically absent volume expanding effect - even at the end of infusion period - this dextran solution cannot be recommended as a blood substitute during acute hemorrhage or as an "expander" in other shock states.

Acetates↗
PubMed1978

A comparison of an ITPA estimated psycholinguistic quotient and WISC-R IQS for developmentally disabled children.

Compared an estimated Psycholinguistic Quotient, based on Cronkhite and Penner's (1975) revised scoring procedure for the ITPA Psycholinguistic Age, to the WISC-R Verbal, Performance, and Full Scale IQs of developmentally disabled children. The Quotient entered into strong relationships with the WISC-R Verbal and Full Scale IQs. The diagnostic significance of these correlations was discussed. The data indicated that the estimated Psycholinguistic Quotient was a valid measure for screening intellectual status in this population.

Adolescent↗
PubMed1978

Left ventricular pump failure associated with diffuse coagulation in experimental intraoperative autotransfusion.

Autotransfusion of blood from the peritoneal cavity of pigs under a regimen of acid citrate dextrose solution 1:10 v/v for reservoir anticoagulation or systemic heparinization with 300 Iu/kg did not affect cardiac performance. Rapid deterioration of left ventricular pump performance was observed when low dose systemic heparin 100 Iu/kg was used. Evidence suggests that this is related to diffuse coagulation.

Animals↗
PubMed1978

An electron microscopic study of the location of peptidoglycan in group A and C streptococcal cell walls.

The morphological appearance of deproteinized Group A and C streptococcal walls after treatment by different procedures extracting teichoic acids and polysaccharides (formamide, hydrochloric acid, nitrous acid, trichloroacetic acid, sulphuric acid, sodium hydroxide and sodium deoxycholate) was compared with the content of teichoic acids and polysaccharides remaining in the treated walls. All procedures extracted teichoic acids almost completely, but polysaccharides were extracted to various degrees. The ultrastructural appearance of walls after these extractions still exhibited the triple-layered wall profile; only a reduction of thickness of the wall and of electron density of the layers occurred. There was no direct correlation between the reduction of rhamnose content and thickness of walls. The ultrastructural localization of peptidoglycan in the streptococcal walls was explored by means of the indirect immunoferritin technique using anti-peptidoglycan antibodies isolated from anti-Group A-variant antisera. Ferritin particles were bound predominantly to filamentous structures which protruded from both surfaces of peptidoglycan fragments and isolated walls. Peptidoglycan was also detected on the filamentous protrusions of whole cocci. These results contradict models of the streptococcal wall in which peptidoglycan forms the innermost layer and support a mosaic structure in which peptidoglycan forms a network of the peptidoglycan-polysaccharide complex.

Antibodies, Bacterial↗
PubMed1978

The characteristics of adsorption of pyrophosphate and citrate onto whewellite.

The adsorption of pyrophosphate and citrate to whewellite was measured by solution depletion. At pH 6.5, the concentration for one-half surface coverage CE(1/2) by pyrophosphate is 15 micrometer. The estimated maximum coverage is 37.5 mumoles per m2. The CE (1/2) for citrate is 4 micrometer, and the estimated maximum coverage is 6.42 mumoles per m2.

Adsorption↗
PubMed1978

[Age-dependent DNA-content of brain, testis and ovar in men and rats (author's transl)].

The DNA-content of brain, testis and ovar of Wistar rats (inbred strain) was quantitatively analysed. We observed no significant differences between the several parts of the cerebrum, but between the cerebrum and the cerebellum. Furthermore we demonstrated alterations (with increase and decrease) of the DNA content of all brain parts as well as of testis and ovar during the course of life of this Wistar rat strain. These results and further investigations of the water content as well as of the absolute and of the relative weight of brains of these rats (in comparison to humans) extend the data and our knowledge about these subjects. It is possible to differentiate growth phases (by means of the DNA assays, which correlate with the morphologically well defined growth phases. Finally these results are fundamentals and presuppositions for investigations of the production metabolism of these organs because the DNA content is a better parameter (reference or relation figure, resp.) than the dry or wet weights and the protein or the nitrogen contents.

Adult↗
PubMed1978

The use of lysozyme-peroxidase-conjugates for the electron microscopic detection of peptidoglycan in the cell wall of streptococci.

Conjugation of lysozyme with horse radish peroxidase by means of glutaraldehyde results in a complex which retains the activities of both enzymes. The incubation of peptidoglycan with lysozyme-peroxidase followed by the reaction with 3,3'-diaminobenzidine and H2O2 results in a strong labelling of both sides. In contrast, after treatment with peroxidase alone no reaction was observed. Thus, the specific binding of lysozyme-peroxidase can be used for the electron microscopic localization of this component in the bacterial cell wall. Isolated peptidoglycane as well as trypsinized cell walls of group A and C streptococci were labelled both on the inner and the outer surface. The surface of intact cells of group A- and C-streptococci was labelled only sparsely. In contrast, by means of the indirect immunoferritin technique strong labelling of intact cells was effected with specific anti-peptidoglycan antibodies. The specificity of these antibodies are mainly directed to the peptide side chains. From this we suggest that in the cell wall of group A and C streptococci the lysozyme-sensitive part of the peptidoglycan is not so superficially localized as the peptides.

Cell Wall↗
PubMed1978

[The localization of the anodically-moving thermolabile antigen (ATA) of gram-negative bacteria with ferritin-labelled antibodies (author's transl)].

In studies on the antigenic structure of shigellae, an anodically-moving thermolabile antigen (ATA) was found, which furthermore could be detected in many other enterobacteriae (9, 10). ATA is a glycoprotein with a high molecular heterogeneity, resulting from aggregates of a subunit with a molecular weight of about 22000 Daltons. In the present paper the antigen was localized on the cell surface of several species by means of the immunoferritin technique. Antibodies against the purified ATA were raised in rabbits and were coupled with ferritin using glutaraldehyde. The antigen was found focally distributed over the whole circumference of the cell. According to the location of the ferritin granules, the ATA is tightly attached to the outer membrane. Especially some rough forms of the bacteria were heavily labelled on their surface. From the results obtained we conclude that in the smooth form the polysaccharide side chains of the somatic antigen cover the ATA.

Antibodies, Bacterial↗
PubMed1978

[Agglutinins in marine brown algae. Dedicated to Professor Dr. H. Knöll on his 65th birthday].

Extracts from the brown algae Fucus vesiculosus, F. serratus, and Halidrys siliquosa agglutinated human erythrocytes of groups A, B, O, and AB as well as erythrocytes from some animal species, ascites tumour cells from mice, and streptococci of many groups of the LANCEFIELD system. The extract of Chorda filum did not show haemagglutination but agglutinated ascites tumour cells and streptococci of a few groups.

ABO Blood-Group System↗