Search PubMed⌕ Search

Biomedical subjects

M Wagner

Publications and source records attributed to M Wagner.

At least 1,081 records · Page 60Linked to original sources
PubMed1978

[Use of ferritin conjugates of a lectin from mistletoe (Viscum album L.) for the electron microscopic localization of cell surface receptors].

Ferritin conjugates of a lectin from mistletoe (Viscum album L.) were used for the electron-microscopic demonstration of carbohydrate receptors on the cell surface of human erythrocytes and murine tumor cells. Human A1 erythrocytes showed only a slight focal binding of ferritin. Cells of the mouse ascites tumor strain L 1210 were labelled very tightly on their surface and incorporate the ferritin by pinocytosis. Furthermore they showed cytotoxic changes in their ultrastructure. In the presence of galactose the labelling on the surface, the incorporation of the conjugate within the cell as well as the cytotoxicity were inhibited.

Animals↗
PubMed1977

[Effects of experimental hypovolaemic shock on platelets in the pig (author's transl)].

The effects of standardized blood loss on the platelets, as well as on coagulation, fibrinolysis and complement parameters were studied in the pig. A simultaneous fall in arterial and venous platelet counts was demonstrated during the initial phase of blood loss, with a concomitat rise in pulmonary vascular resistance. After exsanguination to the extent of about 40% of the blood volume, the arterial platelet counts increased significantly, whilst the pulmonary vascular resistance, which was negatively correlated with the arterial platelet counts throughout the experiment, fell. Venous platelet counts, on the other hand, remained low during this experimental period and were correlated with cardiac output, but not with pulmonary vascular resistance.

Animals↗
PubMed1977

Redistribution and internalisation of anti-AH1e (anti-AHP)- and Concanavalin A-binding sites.

The redistribution and internalisation of two different lectin-binding sites on the same cell was investigated electron microscopically on unfixed rat liver cell cultures. For these purposes an electron microscopic double labeling technique was used for visualisation of the anti-AHel-binding sites by the gold-labeled lectin and for the demonstration of the Concanavalin A-binding sites by the Concanavalin A-peroxidase technique. From the experiments it was evident that the ligand-induced redistribution of the antiAHel-binding sites effects a rearrangement of the Concanavalin A-binding sites. Both markers were found in a clustered distribution on the cell surface. Following the redistribution an internalisation of both lectin-binding sites could be observed on central and peripheral cell parts. In the lateral cell parts an accumulation of the both markers occurred on the plasma membrane.

Binding Sites↗
PubMed1977

Immune response and dental caries incidence in Streptococcus faecalis-monoassociated Harvard caries-resistant and caries-susceptible rats.

Harvard caries-resistant rats (CR) monoassociated with cariogenic Streptococcus faecalis (SF) for 150 days developed early high levels of specific SF serum agglutinins, moderate levels of salivary antibody, and little or no caries activity. Harvard caries-susceptible (CS) SF-monoassociated rats developed serum antibody more slowly and never reached the titers of the CR group. Little or no salivary agglutinin was found, and extensive caries developed. Second-generation monoassociated CR rats (CR-2) born to SF-monoassociated CR parents showed an immune response and caries activity more nearly resembling those of the CS rat than those of their CR parent. The CR rat appeared to be a high responder and the CS rat, a low responder to viable antigen on the mucous surfaces in both serum and salivary antibody production. Immunization by parenteral injection of killed SF cells produced equally high serum titers in both CR and CS strain rats, but failed to increase salivary agglutinin titers in any group or to protect against caries. These data suggest that there may be an immunologic basis for genetic differences in caries susceptibility between CR and CS Harvard rat strains and that this difference has been modified by some factor in the environment of the CR-2 rats.

Agglutinins↗
PubMed1977

Peroxidase and gold complexes of lectins for double labeling of surface-binding sites by electron microscopy.

Double labeling experiments were performed for visualization of the binding sites of Concanavalin A and anti-AHel (the lectin from Helix pomatia). The anti-AHel was labeled with the colloidal gold whereas the membrane bound Concanavalin A was demonstrated by an affinity technique using horseradish peroxidase. The two markers used could be clearly distinguished electron microscopically. The specificity of the cell surface double labeling was demonstrated in the control experiments. A topological distinct localization of the both lectin-binding sites is evident.

Animals↗
PubMed1977

Immunoferritin studies on the multiplication of Sindbis virus in chick embryo fibroblasts.

Replication of Sindbis virus chick embryo fibroblasts was studied by means of the immunoferritin technique. Virus specific antigens, diffusely distributed in the cytoplasm, were observed at first 3 hours p.i. At the same time in ohter cells ferritin particles in linear arrangement occured at the surface of vesicles. At these places heavily labelled nucleocapsids were observed later on. This means, that the surface of vesicles is a site of assembly of nucleocapsids. Maturation of virus takes place at the cell surface as well as at the vesicles by budding. In early stages of replication maturation at the membrane prevalis.

Animals↗
PubMed1977

[The occurrence of cross-reactive antigens in myocardial tissue and in streptococcus pyogenes. IV. Cytotoxic effect of lymphocytes sensitized in vivo to group A streptococci on primary cultures of heart muscle cells (author's transl)].

The cytotoxic effect of rat lymphocytes in vivo sensitized to group A streptococci, rat heart tissue or streptococcal M proteins was investigated in primary cultures of beating rat heart cells. Cytotoxicity was found in 75 to 82% of heart cell cultures in contact with lymphocytes sensitized to streptococcal whole cells or lysates and in 100% of those exposed to lymphocytes sensitized to rat heart tissue. Lymphocytes sensitized to M protein exert a cytopathic effect only in 8 and 12% cultures, respectively, and to a much more limited extent. Controls were affected in 6%. The cytotoxicity test seems to be useful to examine the presence of cross-reactive antigens within streptococcal vaccines.

Animals↗
PubMed1977

Electron microscopic detection of the cryptantigen AhumHP (Friedenreich antigen) on human erythrocytes by means of gold-labelled agglutinin from Helix pomatia.

Using the gold-labelled agglutinin anti-AHP from Helix pomatia (edible snail) the ultrastructural site and distribution of the cryptantigen (Friedenreich antigen) AhumHP was revealed on neuraminidase treated human O and B erythrocytes. On A1 and A2 cells the antigen was demonstrated simultaneously with the blood group antigen A by labelling the cells with anti-AHP-ferritin followed by removing the neuraminic acid and labelling with anti-AHP-gold. The cryptantigen is focally distributed on the cell surface.

Agglutinins↗