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Biomedical subjects

M Wagner

Publications and source records attributed to M Wagner.

At least 1,045 records · Page 58Linked to original sources
PubMed1980

[Indications for osteosynthesis in childhood hand injuries (author's transl)].

The general indication for osteosynthesis in childhood can also be applied to the bones of the hand. The preferred operative procedure is to reduce and stabilize the fracture with one or two percutaneous Krischner-wire. Where there are defects in the bones or soft tissues, the use of a mini external fixation has proved rewarding, even on growing bones.

Adolescent↗
PubMed1979

The effect of DL-2-bromopalmitate on the utilization of palmitic acid by rat granular pneumocytes.

The effect of DL-2-bromopalmitate (BrPA), an analogue of palmitic acid (PA), on the utilization of this fatty acid by rat lungs was investigated by a combination of anatomic and biochemical methods. The experiments were performed in vitro on two types of preparations, isolated perfused lungs and lung slices. In the isolated lung preparation the substrate reached the lung via the capillaries, in lung slices via the alveolar epithelium. Electron microscope autoradiography showed that BrPA depressed uptake of PA by granular pneumocytes. Radioactivity recovered by tissue analysis and capture of CO2 established that PA oxidation and incorporation into phospholipids and triglycerides was depressed by BrPA. A close correlation was found between the reduction in radioactivity in phospholipids and the grain density over lamellar bodies. The study shows that BrPA reversibly interferes with the uptake and utilization of long chain fatty by granular pneumocytes. BrPA appears as a useful tool to study palmitate metabolism and surfactant production by the lung.

Animals↗
PubMed1979

The short-term fixation of radiopharmaceuticals in bone.

The relationship between local blood flow and bone clearance of 18 F, 32 P-P (orthophosphate), 32 P-PPi (pyrophosphate), 45 Ca, 85 Sr, 99mTc- and 113 Sn-EHDP was evaluated in the non-adult rat. During one single blood passage nearly 100% of 18 F was extracted in the femoral diaphysis, the extraction efficiency for the other radiopharmaceuticals was about 40%. Blood flow and bone clearance was higher and the extraction efficiency lower in the distal femoral epiphysis including the growth plate. The short-term bone uptake of 32 P-P was lowered considerably by the simultaneous application of inactive orthophosphate, whereas the bone uptake of 32 P-PPi was not suppressed. It is suggested that the rapid deposition of radiopharmaceuticals in bone is not caused by an ionic exchange process on crystal surfaces of already existing mineral but is due to an ionic exchange during the precipitation of calcium phosphate in the calcifying bone matrix. Only 18 F is exchanged with hydroxyl groups of already precipitated hydroxyapatite, whereas the calcium and phosphate ions in the precipitated hydroxyapatite are not further accessible to an exchange process.

Animals↗
PubMed1979

Construction and characterization of a recombinant plasmid encoding the gene for the thymidine kinase of Herpes simplex type 1 virus.

We have constructed a hybrid plasmid by insertion of the thymidine kinase (TK) gene of Herpes simplex virus (HSV) type I at the BamHI site on Escherichia coli plasmid pBR322. The restriction endonuclease cleavage site map for the viral DNA fragment was determined for ten nucleases, and the insert in the recombinant plasmid has the same restriction nuclease digestion pattern as bona fide viral DNA. This result indicates that the plasmid contains an accurate copy of the viral DNA. The viral TK gene carried on the plasmid can be introduced into mammalian cells where it is expressed. This source of DNA with a selectable marker should be of considerable practical use in gene-transfer experiments in mammalian cells.

Chromosome Mapping↗
PubMed1979

Immunofluorescent and immunoelectron microscopic demonstration of primate heart muscle antigens cross-reactive with Streptococcus pyogenes.

The presence of antigens in heart muscles cross-reactive with antibodies against Streptococcus pyogenes were examined in heart samples of seven primate species (chimpanzee, rhesus macaque, stump-tailed macaque, hamadryas baboon, capuchin monkey, green monkey and patas monkey). Cross-reactive antigens were localized by means of the indirect immunofluorescent technique in nearly all samples tested. The fluorescence pattern was the same as found in the heart muscle of other mammals. Using the indirect immunoferritin technique the substructural distribution of the antigens was investigated in heart muscle of rhesus macaque and hamadryas baboon. Beside the location on the cell surface the antigens were also demonstrated within the cell. Above all there was a strong labelling along the intercalated discs and in the sarcoplasmatic reticulum.

Animals↗
PubMed1979

Nutritional supplementation and the outcome of pregnancy. I. Birth weight.

Colombian women at risk of malnutrition were enrolled in a health care program and randomly assigned to supplementation and control groups at the beginning of the third trimester of pregnancy. The net dietary intake increments resulting from supplementation amounted to 155 cal and 20 g of protein per day. Supplementation had a significant effect on the mean birth weight of male infants, but not that of female infants; the mechanisms responsible for the sex differences remain to be elucidated. The randomized trial design of the experiment and the documented similarity between the experimental and control groups at the onset permit the conclusion that the observed differences were caused by the food supplementation program. The effect of supplementation on maternal weight gain and the association of the latter with birth weight strongly suggest that improved maternal nutrition mediated the effect on birth weight.

Birth Weight↗
PubMed1979

Nutritional supplementation and the outcome of pregnancy. II. Visual habituation at 15 days.

Colombian women at risk of mild-to-moderate malnutrition were enrolled in a health care program and randomly assigned into nutritional supplementation and control groups at the beginning of the third trimester of preganancy. One hundred unsupplemented and 144 supplemented infants were tested at 15 days of age: a 2X2 checkerboard was presented eight times followed by a single presentation of a 6X6 checkerboard. Unsupplemented infants showed less initial attention followed by slower habituation and higher levels of movement than the supplemented infants. The observed effect of maternal nutritional supplementation during the last trimester of pregnancy on new-born attention was interpreted to be maturational in nature.

Apgar Score↗
PubMed1979

Two-years' study with a combination of pindolol and clopamide ('Viskaldix') in patients with moderate hypertension.

A study was carried out to evaluate the long-term effects and side-effects of a combination product containing the beta-blocker pindolol (10 mg) and the diuretic clopamide (5 mg) in 15 patients with moderate hypertension. All patients completed the 2-years' study. The dose of the combination was increased until blood pressure normalized or a maximum dose of 3 tablets (equivalent to 30 mg pindolol and 15 mg clopamide) daily was reached. Blood pressure and heart rate were recorded monthly and detailed medical examinations were done regularly throughout the study. A mean dose of 2 tablets of the combination product (20 mg pindolol and 10 mg clopamide) produced a significant reduction in blood pressure. In all but 1 patient, blood pressure control was achieved and maintained. No tolerance developed. Heart volume showed a marked decrease. No side-effects of clinical importance were noted.

Adult↗
PubMed1979

Immunoelectron microscopic localization of T proteins in the cell wall of Streptococcus pyogenes.

T proteins of Streptococcus pyogenes (group A streptococci) were localized by means of immunochromatographical isolated anti-T-antibodies. For the electron microscopical detection both the direct and the indirect immunoferritin techniques were used. The arrangement of the ferritin particles showed, that the T proteins are evenly distributed on the whole cell surface. They are immediately bound to the outer layer of the cell wall or to only short filaments. On isolated cell walls the T protein was detected only on the outer surface.

Agglutination Tests↗
PubMed1979

Interaction of wheat-germ agglutinin with streptococci and streptococcal cell wall polymers.

Wheat-germ agglutinin agglutinates all strains of group A and group C streptococci, a part of the strains tested of groups B and E and some strains of other streptococcal groups. It also agglutinates peptidoglycan of several streptococcal groups and precipitates some group-specific carbohydrates of streptococcal cell walls as well as some teichoic acid extracts. The basis for agglutination and precipitation of streptococci and streptococcal cell wall polymers appears to be the interaction of the combining sites of the agglutinin with N-acetyl-D-glucosamine residues present in the cell wall polymers, either in terminal or in internal positions respectively.

Acetylglucosamine↗
PubMed1979

[Childhood injuries treated at a university clinic for traumatology (author's transl)].

The accidents to all children up to the age of 14 years admitted to the I. Universitätsklinik für Unfallchirurgie in Wien in 1976 (2977 children) were analysed as to age-distribution, cause of accident, type and location of injury. There was an increase in accidents to older children. 13% of the injuries were due to traffic accidents; 60% occurred in the course of play; 27% on the field of sport. More than 60% were only minor injuries such as abrasions, lacerations, crushed fingers and toes etc., 30% bone fractures and ligamental injuries; 2,5% brain traumas. Most of the bone fractures were localized iment and no lethal injuries.

Accidents↗