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Biomedical subjects

M Tardieu

Publications and source records attributed to M Tardieu.

At least 163 records · Page 9Linked to original sources
PubMed1986

[A spectacular and benign syndrome of neonatal convulsions: deep sleep myoclonus].

Four case reports concerning neonates presenting, without any predisposing factors, severe and lasting myoclonus, occurring exclusively during sleep. These began between 1 and 4 days of age and stopped between 3 weeks and 5 months. Electro-encephalogram was normal, even during episodes of clonus which occurred during deep sleep. A predisposing genetic factor is likely. A better knowledge of this syndrome should avoid hospitalizations and misuse of anticonvulsive treatments.

Electroencephalography↗
PubMed1986

[Acquired immunodeficiency syndrome in an adolescent hemophiliac].

In an adolescent with hemophilia B, the diagnosis of AIDS was established in face of cachexia, orodigestive candidiasis, associated with lymphopenia, major decrease in T4/T8 ratio with marked decrease in T helper cells and presence of LAV-antibodies. The patient died rapidly from a cerebral infection due to Toxoplasma gondii. Difficulties in diagnosis and treatment of cerebral toxoplasmosis are discussed.

Acquired Immunodeficiency Syndrome↗
PubMed1985

Detection of measles virus RNA in lymphocytes from peripheral-blood and brain perivascular infiltrates of patients with subacute sclerosing panencephalitis.

To clarify the relation between lymphocytes and measles virus in subacute sclerosing panencephalitis, we used in situ hybridization and a cloned measles virus DNA probe, specific for nucleocapsid protein, to detect measles virus RNA sequences in circulating lymphocytes and brain perivascular cuffs of patients with subacute sclerosing panencephalitis. Seventy to 90 per cent of peripheral mononuclear cells from three such patients were found to contain measles virus RNA sequences. In contrast, only a few infected cells were observed in four seropositive adults (0.1 to 5 per cent) and three age-matched children (10 to 15 per cent) used as controls. In one sample of brain tissue from a patient with subacute sclerosing panencephalitis, viral RNA sequences were also detected in nerve cells and in numerous cells from the perivascular infiltrates. In contrast, no hybridization was observed in brain tissue from a patient with herpetic encephalitis and from a patient with postlymphoma encephalitis. We conclude that measles virus has a strong tropism for lymphocytes and nerve cells in subacute sclerosing panencephalitis and that lymphocytes may be involved in the pathogenesis of the disease.

Adult↗
PubMed1985

Intrathecal synthesis of different alpha-interferons in patients with various neurological diseases.

CSF and sera from 238 newborns and children with various neurological diseases were assayed on bovine cells for the presence of alpha-interferon (IFN). An intrathecal synthesis of pH 2-resistant alpha-IFN was recovered in all newborns and in more than 90% of children with herpes encephalitis. It was also observed in one case of mumps encephalitis and in one case of encephalitis associated with Influenza A infection. An acid-labile alpha-IFN production was detected in CSF from more than one half of patients with viral meningitis or active congenital rubella and in those with neurological complications of systemic lupus erythematosus. This alpha-IFN subtype was also detected in CSF from only 2/37 children with measles encephalitis. In contrast, no alpha-IFN (less than 2 IU) in CSF was found among patients with subacute sclerosing panencephalitis, Guillain-Barré syndrome, Reye's syndrome, acute cerebellar ataxia, infantile spasms or facial paralysis of unknown origin.

Acute Disease↗
PubMed1984

Autoimmunity following viral infection: demonstration of monoclonal antibodies against normal tissue following infection of mice with reovirus and demonstration of shared antigenicity between virus and lymphocytes.

Splenic lymphocytes from adult C57BL/6 mice infected with purified reovirus type 1 or 3 particles were fused with NS1 myeloma cells. Approximately 300 clones were obtained from each fusion (type 1 or type 3) and the supernatants from these clones were screened by radioimmunoassay for their ability to bind virus, T lymphocytes, brain, liver, lung tissues and isolated oligodendrocytes and ependymal cells. Approximately 10% of clones (33 and 26 clones, respectively) were positive for each fusion. For reovirus type 1:21% of positive clones bound only virus, 64% bound one of the normal tissues but not virus, and 15% bound both virus and one or more of the normal tissues. For reovirus type 3: 19% of positive clones bound only virus, 73% bound normal tissue only, and 8% bound both virus and normal tissue. Only 3 positive clones were obtained from uninfected control animals. These experiments demonstrate that (a) during the course of an immune response to a virus, autoantibodies are generated which react with a large variety of normal tissues and that (b) there are shared antigenic structures between viral determinants and normal tissue that can be identified by monoclonal antibodies. Although these results suggest two mechanisms by which an autoimmune response may develop following viral infection, the biological significance of these autoreactive monoclonal antibodies remains to be elucidated.

Animals↗
PubMed1984

Locked in syndrome with a favourable outcome.

The locked in syndrome seldom occurs in children, is rarely due to trauma and only in exceptional cases has a favourable outcome. The case reported below is unusual in all these respects, and its evolution might be an example of successful axonal regeneration in the central nervous system.

Accidents, Traffic↗
PubMed1984

[Sex-linked, nonprogressive, familial chorea].

In a family, 4 patients on 3 generations have a non progressive chorea. One of them is epileptic and mildly retarded. Transmission appears to be X linked and recessive. This pattern introduces a new difficulty in the L dopa test for genetic prognosis.

Adolescent↗
PubMed1984

[Acute hemiplegia complicating Kawasaki disease].

An infarct in the left middle cerebral artery territory in a 9 months old girl occurred in the course of Kawasaki's disease which in addition had induced a coronary aneurysm and a myocardial infarct. Embolism from the myocardial infarct was likely to have provoked the cerebral infarction. Neurological symptoms and signs are unusual in Kawasaki's disease.

Acute Disease↗
PubMed1983

Generation of a monoclonal antibody (Epenl) which binds selectively to murine ependymal cells.

In order to define surface antigens unique to ependymal cells, spleen cells from C57/B16 mice immunized with a suspension of 70-80% purified isolated ependymal cells from syngeneic animals were fused with NS-1 myeloma cells. Five hybridomas were found which secrete monoclonal antibodies that recognize ependymal cells both by indirect immunofluorescence and radioimmunoassay. One of them, Epenl, appears to be a relatively specific surface marker of murine and rat ependymal cells, whereas the 4 others also recognize neurons, astrocytes, and/or oligodendrocytes. Absorption of Epenl with murine cerebral cortex did not affect its binding, whereas absorption with ependymal cells abolished it. Labeling of in vivo sections with Epenl demonstrates prominent binding to ependymal cells lining ventricular cavities. Epenl does not bind to neurons or astrocytes in culture, and binds only minimally to isolated oligodendrocytes. It does, however, recognize an antigenic determinant present in lung tissue.

Animals↗
PubMed1983

Age dependent susceptibility to Reovirus type 3 encephalitis: role of viral and host factors.

Reovirus type 3 inoculated intracerebrally or subcutaneously into newborn mice induced an acute necrotizing and uniformly fatal encephalitis. Subsequently, between the eighth and tenth day of age, reovirus type 3 infection changed to a nonlethal infection. This pattern of virulence was directly correlated with the ability of the virus to replicate in the brain and histologically was accompanied by a gradual diminution in the intensity and extent of encephalitis such that histological abnormalities were absent in the brains of virus-injected adult animals. Some animals injected at ages 10 to 18 days developed brainstem and diencephalic lesions, indicating a nonuniform resistance of neurons to viral infection with aging. Neither a changing immune response pattern nor a modification or loss of viral receptors appears to explain either the susceptibility of newborn or the resistance of adult animals to reovirus type 3 encephalitis. Splenic mononuclear cells from mice younger (but not older) than 7 days also permitted reovirus type 3 replication in vitro. Thus, the age dependent resistance to reovirus type 3 encephalitis appears related to an intrinsic resistance to viral replication by neuronal cells, a resistance that may occur simultaneously in nonneuronal cell populations.

Age Factors↗
PubMed1982

Viral receptors on isolated murine and human ependymal cells.

Viruses that infect ependyma cause ependymitis in humans and hydrocephalus in experimental animals. We report that reovirus type 1 (which induces hydrocephalus in mice) binds to the surface of isolated human and murine ciliated ependymal cells. With the use of recombinant viral clones, the binding property was mapped to the type 1 viral hemagglutinin, which also determines in vivo the affinity of reovirus type 1 for ependyma. Mumps virus, measles virus, parainfluenza type 3, and herpes simplex virus type 1 bind to murine ependyma cells, whereas reovirus type 3, herpes simplex virus type 2, and poliovirus type 2 do not.

Animals↗
PubMed1982

Identification of a hemagglutinin-specific idiotype associated with reovirus recognition shared by lymphoid and neural cells.

A xenogeneic antiserum raised to antireovirus immunoglobulin was used to define an idiotypic determinant present on antibodies to reovirus type 3 hemagglutinin. The same idiotype was identified on nonimmune lymphoid cells and on neuronal cells that specifically bind the hemagglutinin of type 3 reovirus. This idiotypic determinant, called Id3, is shared by (a) a monoclonal antibody to the neutralization site of hemagglutinin from type 3 reovirus; (b) BALB/c serum antibodies to the hemagglutinin of reovirus type 3; (c) R1.1, a murine thymoma cell line that binds reovirus type 3; (d) primary cultures of murine neuronal cells. The presence of an idiotype shared by antihemagglutinin antibodies and by structures on nonlymphoid cells suggests a general relationship between disparate receptors that recognize a common determinant. Furthermore, this suggests a novel approach for the study of viral receptor interactions and for analysis of mechanisms of autoimmune responses.

Animals↗
PubMed1982

Ependymitis, leukoencephalitis, hydrocephalus, and thrombotic vasculitis following chronic infection by mouse hepatitis virus 3 (MHV 3).

Mouse hepatitis virus 3 (MHV 3) is either avirulent (resistant mice), hepatotropic (susceptible mice), or neurotropic (semisusceptible mice), depending on the strain of mice infected. In semisusceptible mice, infection led first to a transient meningitis, ependymitis, and leukoencephalitis, followed by a permanent communicating hydrocephalus and, later on, to a chronic thrombotic vasculitis affecting meningeal and parenchymal vessels at the brain stem level. Small foci of ischemic necrosis related to vascular occlusions were seen in the dorsal brain stem. Cyclophosphamide treatment of semisusceptible mice significantly reduced the meningeal infiltrates but did not prevent the development of hydrocephalus and other neuropathologic changes. Identical lesions occurred in fully susceptible mice infected with a low dose of virus, but no neurologic disorder could be induced in genetically resistant mice even following immunosuppression or intracranial inoculation. The leukoencephalitis differed from the demyelinating lesions observed with MHV 4. Vascular lesions were of particular interest. More attention should be given to the possibility of virus induced chronic cerebral vasculitis in man.

Animals↗