[Topical anesthetic effects of ethylaminobenzoate on the blink reflex of the cornea of male guinea pigs and on human gingiva].
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Biomedical subjects
Publications and source records attributed to M Tajima.
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The first case was a congenital renal arteriovenous fistula in a 33-year-old woman and the second case was a secondary renal arteriovenous fistula in a 67-year-old man. Both cases were seen with asymptomatic gross hematuria without any cardiovascular symptoms. In case 1, the arterial stage of right selective renal angiogram demonstrated a cirsoid type arteriovenous fistula in the right kidney; then, partial nephrectomy was performed. Angiogram of case 2 demonstrated arteriovenous fistula in the lower part of the right kidney. In this case arterial embolization was performed. After reviewing the literature, we believe that partial nephrectomy or arterial embolization is the most useful operative procedure to treat almost all cases of the cirsoid type renal A-V malformation. Conservative treatment of arteriovenous fistula has increased during the post 10 years.
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Adhesion of Haemophilus paragallinarum to chicken embryo fibroblasts (CEF) in vitro was investigated in correlation with its virulence. Under the scanning electron microscope, the organisms were seen adhering to CEF exposed to the bacteria. By transmission electron microscopy, the organisms appeared to attach to the plasma membrane of CEF by their fuzzy material. They were enclosed in membrane-limited vesicles and appeared morphologically intact. Specific fluorescence was seen in the cytoplasm of CEF in later stages of infection. There was a good correlation between pathogenicity of the organisms for chickens and their ability to adhere to CEF. This correlation was reinforced by the fact that the ability of a pathogenic strain to adhere to the mucosal surface of the chicken trachea was demonstrated by scanning electron microscopy.
A ruthenium red-staining capsule was observed on two pathogenic strains, but not on one nonpathogenic strain of Mycoplasma gallisepticum. The capsule appeared to mediate cytadsorption of mycoplasmas to the chicken tracheal epithelium without evidence of membrane fusion. No relationship was seen between the presence of capsule and hemagglutination titers of the strains examined.
An in vivo-passaged strain of Mycoplasma hyopneumoniae attained viability titers of 10(6) to 10(8) color-changing units per mg of tissue in pig lungs and caused gross and histological pulmonic lesions. Mycoplasmas were readily located in the lumina of the respiratory tract by electron microscopy. In sections of tissue fixed in glutaraldehyde-osmium, the organisms were found to possess many radial fibrils on the outer surface of the limiting membrane. These fibrils appeared to interconnect adjacent mycoplasmas and to extend between the organism and epithelial cell. Ruthenium red staining demonstrated a thick, dark layer of capsular material enveloping the entire mycoplasma cell. The capsular material was seen to bridge the space between the mycoplasma and host cell. The general morphology of the in vitro-passaged strains grown in broth medium was essentially similar to that of the in vivo-passaged strain. In these organisms, however, no long fibrils were seen, although a fuzzy layer was present outside the cell membrane. The ruthenium red-positive capsule was stained less intensely, and its width was only about one-half that observed for the in vivo-passaged strain. In negatively stained preparations, the cells had an outer fringe of amorphous material apparently corresponding to the fuzzy layer seen in thin sections. The in vitro-passaged strain grew poorly in pig lungs and lost its ability to produce gross pulmonic lesions. The organisms in the respiratory tract had a capsule much thinner than that of the in vivo-passaged strain.
Fifty-four newborn infants with myelomeningocele and hydrocephalus were studied by ventriculography using water-soluble contrast media; 20 were also studied by metrizamide myeloencephalography and computed tomographic (CT) cisternography. Ventriculography suggested that the aqueduct was patent in all cases. Outflow of contrast medium from the fourth ventricle was slow in most cases, complete obstruction was seen in 15%, communication was delayed at the outlet in 54%, and rather free communication was observed in 31%. Metrizamide myeloencephalography and CT cisternography suggested a partial block at the level of the ambient cisterns in approximately one-third of infants. These findings support the concept that flow of cerebrospinal fluid is reduced in several areas. Aqueductal stenosis was not considered an important factor in hydrocephalus, while the most important site of obstruction was felt to be the lowest portion of the fourth ventricle.
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Diagnostic cytology may be of use for some information in the field of cancer treatment. From the view point of morphological characteristics of cancer cells which are obtained before treatment, we can presume the prognostic courses in certain level. For example, presumption of prognosis on 420 cases with uterine cervix carcinomas according to their cytological findings before therapy (Cobalt irradiation), showed 77% in its accuracy rate. Particularly 144 cases, which were classified to FIGO Stage III were tested by cytological method, accuracy rate of prognostic presumption showed 76%. On the other hand, presumption of prognosis on same cases by means of histological method showed 68% in its accuracy rate. On the base of morphological change of cancer cells, therapeutic effects can be checked during the treatment. Reoccurrence may be recognized in early stage when any subjective or clinical symptoms manifest. Alteration of cell components, except cancer cell, shows sometimes a relation with clinical course. In pleuroperitoneal effusions, numerical alterations of macrophage and lymphocyte are valuable factors which relate to clinical course and outcome of cancer patients.
The neurological and neuroradiological manifestations of pediatric achondroplasia are analyzed on the basis of 10 cases. In addition to the classical symptomatology of an enlarging head, with or without increased intracranial pressure, several patients presented symptoms related to a small foramen magnum. Respiratory problems and quadriparesis were also observed in these patients. Six patients who were treated by foramen magnum decompression showed remarkable improvement. Neuroradiological evaluation revealed a significant number of patients with dilated cortical sulci and basal cisterns, and mild dilatation of the ventricles on computerized tomography (CT) and/or ventriculography. Two patients showed signs of obstruction at the fourth ventricle outlets. Some exhibited anomalous dilatation of cerebral veins and dural sinuses, narrowing of the sinuses at the jugular foramen, and enlarged emissary veins. Ventriculoperitoneal or ventriculoatrial shunts were placed in three patients. In the other children with a large head and dilated ventricles, head growth curves paralleled the normal slope, and there was no significant clinical evidence of increased intracranial pressure or enlarging ventricles on follow-up CT scan.
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