Search PubMed⌕ Search

Biomedical subjects

M Satoh

Publications and source records attributed to M Satoh.

At least 1,153 records · Page 64Linked to original sources
PubMed1981

Identification of a serum glycoprotein that increased in OK-432-treated mice as hemopexin.

LB, the antitumor protein whose level is increased in the serum of OK-432-treated mice, was identified as hemopexin, which is a serum beta-glycoprotein having a heme-binding capability. When incubated with heme, LB showed several absorption maxima in the ultraviolet and visible regions, and the addition of dithionite increased the extinction of the Soret band, which was shifted to a longer wavelength. The ultraviolet circular dichroism spectrum of LB exhibits a positive maximum at 292 nm and a shoulder near 280 nm. The far ultraviolet CD spectrum of LB has a prominent positive maximum at 231 nm. These spectral characteristics are identical with those of rabbit, rat or human hemopexin. In addition, the relative mobility on polyacrylamide gradient gel electrophoresis and the results of amino acid analysis ae in very good agreement with results obtained previously for rabbit and human hemopexins. Since LB has enhancing effects on the tumor cell killing and binding by OK-432-elicited macrophages, these results strongly imply that the increase of hemopexin in serum plays an important role in the host-mediated antitumor activity of certain antitumor drugs.

Amino Acids↗
PubMed1981

Antitumor and toxohormone-neutralizing activities of human ceruloplasmin.

Human ceruloplasmin was found to have a neutralizing effect against the toxohormone activities of the basic protein isolated from Ehrlich carcinoma cells; this basic protein decreases the levels of serum iron and liver catalase activity upon intraperitoneal injection into mice and shows direct cytotoxicity toward normal mouse lymphocytes and macrophages. Furthermore, it was also shown that ceruloplasmin has antitumor activity against sarcoma-180 cells implanted in ICR mice.

Animals↗
PubMed1980

Analgesic action of intrathecal and intracerebral beta-endorphin in rats: comparison with morphine.

beta-Endorphin and morphine were given into the nucleus reticularis gigantocellularis (NRGC), spinal subarachnoid space and third ventricle of rats, and the antinociceptive effects were assessed by the tail-pinch method. The NRGC was 4-5 times as sensitive to beta-endorphin as was the spinal subarachnoid space and third ventricle, while the NRGC was 13-19 times as sensitive to morphine as were the other regions. Thus, it would appear that beta-endorphin has more sites of action for the production of analgesia than does morphine.

Analgesics↗
PubMed1980

Effects of tyrosyl-arginine (kyotorphin), a new opioid dipeptide, on single neurons in the spinal dorsal horn of rabbits and the nucleus reticularis paragigantocellularis of rats.

The effects of a new endogenous opioid dipeptide (Tyr-Arg), kyotorphin, on single unit activities recorded from the lamina V type neurons in the spinal dorsal horn and the neurons in nucleus reticularis paragigantocellularis (NRPG) of the medulla oblongata were investigated in the rabbit and rat, respectively. Microelectrophoretically applied kyotorphin predominantly depressed the lamina V type neurons but excited the NRPG neurons. Such predominant effects were antagonized by naloxone. These results suggest that kyotorphin has qualitatively similar actions to those of enkephalins in both central regions examined.

Animals↗
PubMed1980

A serious problem of the heated thermocouple flowmeter.

In the measurement of regional blood flow with heated thermocouple flowmeter, we frequently observed puzzling phenomenon that the measured values were augmented while the flow was actually diminished and vice versa (Inversion). "Inversion" was directly attrebutable to the structure of the thermocouple probe. A countermeasure against "Inversion" was found to lie in the arrangement of the hot junction in the center of the heater, and in practical application, it must be checked by stopping the flow at each measurement. Overcoming this technical liability should pave the way for a marked improvement in the reliability of this method which, despite possessing many excellent characteristics for determining regional blood flow, is not widely used.

Animals↗
PubMed1980

Active and inactive renin after SQ 14225 (Captopril) administration.

The changes of active and inactive renin in plasma after the oral administration of 25 mg or 50 mg of SQ 14225 (Captopril) were studied in 29 hypertensive patients. Inactive renin was calculated as plasma renin activity (PRA) after cold storage minus PRA before cold storage. The patients were divided into 2 groups, responders and non-responders, according to the response of active renin to Captopril. In 9 responders, the active renin increased markedly while the inactive renin decreased. On the other hand, in 20 non-responders, both renin activities increased only slightly. These results suggest that inactive renin may be converted in vivo to active renin by Captopril.

Administration, Oral↗
PubMed1980

Purification and characterization of a mouse serum glycoprotein increased in level by administration of an antitumor polysaccharide, lentinan.

One of the three kinds of mouse serum proteins increased in level by the administration of lentinan, designated LA, was found to be ceruloplasmin (p-diphenol:O2:oxidoreductase; EC 1.14.18.1) which contains copper atoms and has amine oxidase activity. This mouse ceruloplasmin is a glycoprotein with a molecular weight of approximately 135,000, and its chemical composition is very similar to that of human ceruloplasmin.

Amino Acids↗
PubMed1980

Enhancement of macrophage tumoricidal activity by a serum glycoprotein that increased in OK-432-treated mice.

An antitumor protein that increased in the serum of OK-432-treated mice showed an enhancing effect on the tumor cell killing by OK-432-elicited macrophages as measured by the 51Cr release test. This protein, designated as LB, also enhanced the cytolytic effect of macrophages on normal target cells, although the activity was lower toward normal cells than tumor cells. On the other hand, LB exhibited no effect on normal, thioglycolate-elicited, or heat-inactivated OK-432-elicited macrophages. The effect of LB on the binding of tumor cells to macrophage monolayers was also examined. On the addition of LB, OK-432-elicited macrophages became capable of binding more tumor cells than before. As in the cytolytic assay, the increased binding by the macrophages was not selective for tumor cells, and normal macrophages did not increase the binding of tumor cells upon the addition of LB. These results indicate that LB enhances macrophage-mediated cytotoxicity by increasing intimate binding between the OK-432-elicited macrophages and their target cells.

Animals↗