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Biomedical subjects

M Rytter

Publications and source records attributed to M Rytter.

At least 55 records · Page 3Linked to original sources
PubMed1984

[Studies on the mycotic and bacterial risk of contamination and the use of nipagin in the artificial insemination of cryosperm].

Primary bacterial and mycological contamination was studied in random human ejaculates. After various phases of cryopreservation secondary microbiological contamination was investigated. Furthermore, semen samples of several donors were inoculated with suspension of different concentrations of yeasts and with the test bacteria Escherichia coli K12 and Staphylococcus aureus SG 511. Microbiological results were then compared before an after cryopreservation. The cryopreserving process was carried out with and without antibiotics to test the antibacterial effectiveness of antibiotics under these conditions. Moreover, we investigated the usability of a chemical preservative (Nipagin) at cryopreservation. The following results were obtained: 9.8 per cent of samples showed primary mycological contamination (1.9 per cent with Candida albicans). Cryopreservation reduced the concentration of fungi by more than 90 per cent on average. The bacteriological investigations have shown, that with one exception the semen was without pathogenetic bacteria. This situation was altered scarcely during the cryopreserving process (also by use of cryoprotective medium without antibiotics). Out of 25 ejaculates 11 had primary non-pathogenetic bacteria. Test bacteria inoculated into semen were not influenced by the addition of antibiotics to the cryoprotective medium (CPM). Nipagin prevented respectively reduced a bacterial or a mycological contamination of the CPM during a storage of 14 days dependent of nipagin concentration but reduced the motility of human spermatozoa after cryopreservation. The results suggest the conclusion to prefer a portionate storage without nipagin at a temperature of nearly + 1 degree C, thereby the microbiological contamination may be neglected.

Anti-Bacterial Agents↗
PubMed1983

Microbiological investigations on cryopreserved human semen.

Primary mycological contamination was studied in 565 random human ejaculates. After sperm preservation at low temperature, secondary mycological contamination of 55 of the ejaculates was investigated. 25 ejaculates were cryopreserved with and without antibiotics and examined bacteriologically before and after different phases of cryopreservation. Furthermore, semen samples of several donors were inoculated with suspensions of different concentrations of yeasts and with the test bacteria Escherichia coli K 12 and Staphylococcus aureus SG 511. Microbiological results were then compared before and after cryopreservation. The following results were obtained: 1.9.8% of samples showed primary mycological contamination (1.9% with Candida albicans). 2. Cryopreservation reduced the concentration of fungi by more than 90% on average. 3. Only one semen sample was contaminated with bacteria (Staphylococcus aureus haemolyticus and Streptococcus nonhaemolyticus). Non-pathogenic bacteria were found in 11 ejaculates (Micrococcus luteus in 5 samples and Staphylococcus epidermidis in 6 samples), and contamination was largely unaffected by cryopreservation, even in the presence of antibiotics. 4. Test bacteria inoculated into semen were not influenced by the addition of antibiotics to the cryoprotective medium.

Anti-Bacterial Agents↗
PubMed1983

[Hemangiosarcoma of the scalp].

Report on a 66-year-old patient with a hemangiosarcoma of the scalp which is known to be the preferred site of this type of tumor. The histologically partly benign hemangioma-like appearance contradicts the progredient clinically malignant and deleterious course of the disease which could not be stopped even by immediate radical large excision of the tumorous tissue with following plastic covering.

Aged↗
PubMed1982

Hapten conjugation in the leucocyte migration inhibition test in allergic chromate eczema.

We studied the in vitro conjugation of chromium salts and found that trivalent chromium binds to protein 10 times more strongly than hexavalent chromium. We also investigated the use of two different lymphocyte migration inhibition tests for the diagnosis of chromium allergy. The capillary method proved superior to the Clausen technique, and potassium dichromate was shown to be a better antigen than chromium chloride. The test results correlated well with standard patch tests and we recommend the capillary method for the investigation of difficult cases of suspected chromium allergy.

Cell Migration Inhibition↗
PubMed1982

[Net K+(86Rb+) uptake of human lymphocytes following phytohemagglutinin stimulation].

We tried to develop a short-time incubation method for the early detection of lymphocyte stimulation due to lectins and antigens. For this purpose the netto-K+(86Rb+)- uptake of (1) nonstimulated, (2) phytohaemagglutinin (PHA) activated and (3) potassium dichromate (K2Cr2O7) activated human lymphocytes was examined by means of 86Rb+ as a marker. The relative netto-K+-uptake was reduced by 38 per cent (p less than or equal to 0.01) on average after the incubation with PHA. After the incubation with K2Cr2O7 the 86Rb+-values differed, however, only unessentially from the nonstimulated controls in one patient sensibilized by K2Cr2O7. We concluded from our results that this method is not suitable for the early detection of cell mediated immune reactions.

Humans↗