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Biomedical subjects

M Ohta

Publications and source records attributed to M Ohta.

At least 1,243 records · Page 69Linked to original sources
PubMed1982

[Angiographic evaluation of mitral valve prolapse: my diagnostic criteria].

Diagnostic method of mitral valve prolapse (MVP) by left ventriculography was proposed. The results of the study are summarized as follows: 1) MVP was assessed by the dislocation of the mitral leaflets from their coaptation site. Diagnosis by left ventriculography was made by the characteristic "hook-like" finding at the coaptation site in the lateral view of the long axial oblique projection (LAP). MVP was classified into three groups by the degree of dislocation. 2) One hundred and twenty-eight patients with secundum atrial septal defect were examined. The diagnosis of MVP was agreed between cineangiography and real-time two-dimensional echocardiography in all the cases except two. On the other hand, by the right anterior oblique view, the diagnostic accuracy was at most 93%. 3) Cineangiographic diagnosis of MVP should be made by the tangential view to the mitral annulus. As to this point, RAO view has various problems and in the previous reports there seems to be some misinterpretations about the prolapse of this valve. For this tangential view, the lateral view of LAP is so useful that MVP must be assessed by this view.

Cineangiography↗
PubMed1981

The amino acid sequence of toxin D isolated from the venom of Indian cobra (Naja naja).

A neurotoxin, designated toxin D, was isolated from the venom of Naja naja by gel-filtration on Sephadex G-50 followed by CM-cellulose chromatography. The amino acid sequence of toxin D was determined by analyzing tryptic and chymotryptic peptides of the reduced and S-carboxymethyl derivative. The venom contains at least four long (toxins A, B, C and D) and two short neurotoxins (toxins I and II) that account for about 16% of the lyophilized crude venom by weight. Toxin D consists of 71 amino acid residues in a single peptide chain cross-linked by five intramolecular disulfide bridges. The lethal toxicity of toxin D is found to be about half those of toxins A, B and C in the venom. Analysis of the amino acid sequence reveals that toxin D differs from toxin A in having -Arg-31, -Glu-35 and -Lys-49 in place of -Ser-31, -Lys-35 and -Arg-49 residues.

Amino Acid Sequence↗
PubMed1981

A possible association of Y chromosome heterochromatin with stature.

A correlation between Y chromosome length and stature was statistically analyzed in a normal male population of 142 Japanese students with a mean age of 24.0 years. Evidence was obtained that increased length of the heterochromatic band Yq12 may be associated with increased height: The correlation coefficient between band Yq12 length and height was 0.17, statistically significant at the 5% level. And, taller males had longer Y chromosomes, in which the mean length of band Yq12 was significantly longer than that of shorter males. No correlation was seen between length of the euchromatic band Yq11 and stature. The present study reveals a possible effect of Yq heterochromatin on the development of body height in man.

Adult↗
PubMed1981

Rigid spine syndrome: clinical and histological problems.

A case is described with the characteristic clinical features of rigid spine syndrome: childhood onset in a male, very slowly progressive weakness and muscle atrophy, limitation of neck and trunk flexion, joint contractures, normal intelligence, no clear family history, increased serum CPK levels and myopathic patterns in EMG and muscle biopsy specimens. Myopathic patterns with various histological changes in muscle biopsy specimens were seen in all eight reported cases of the rigid spine syndrome, but there are no characteristic histological findings in the rigid spine syndrome.

Adolescent↗
PubMed1981

Glial fibrillary acidic protein in medulloblastoma.

Twenty-four cases of classical medulloblastoma and one case of desmoplastic medulloblastoma were examined for glial fibrillary acidic protein (GFAP) using the immunoperoxidase method to assess astrocytic differentiation. In 16 cases of classical medulloblastoma GFAP-positive cells were present in variable numbers. These cells were classified as three different types according to size and shape. The type 1 cell was morphologically identical to the ordinary tumor cell, with a hyperchromatic nucleus and a scanty cytoplasm. The type 2 cell had a fairly rich cytoplasm with short cytoplasmic processes. The type 3 cell was characterized by a relatively large nucleus with sparse chromatin and well-developed cytoplasmic processes, and was considered a reactive astrocyte. The type 1 and some of the type 2 cells seemed to be neoplastic, displaying astrocytic differentiation. The remaining type 2 cells may have been reactive astrocytes. In one case of desmoplastic medulloblastoma, the majority of GFAP-positive cells were arranged in "islands", and had delicate fibrillated processes. GFAP-positive cells were also observed outside these "islands", though they were less numerous. Most of them were regarded as type 3 cells, but some were type 2. This may be interpreted as meaning that the glial character of the tumor was expressed more within than outside these "islands".

Adolescent↗
PubMed1981

Alternate culture and animal passage of human glioma.

The method of the alternate culture and animal passage was introduced in the study of human glioma. For animal passage the hereditary asplenicathymic (lasat) mice were used as a carrier. Because the lasat mice have practically no cellular and only little humoral immunity, the rate of tumor take was expected to be raised, and successful results were obtained. Ultrastructural and immunohistochemical (GFA protein) studies were also done. The overgrowth of stromal elements in reculture of tumors in lasat mice was less vigorous than in athymic nude mice. After four passages through lasat mice, an established well differentiated cell nests, and this alternate culture and animal passage suggested to enhance the differentiation and growth capacity. After three passages through lasat mice, the tumor line, KNS-42-L, produced tumors also in athymic nude mice and their histological features were essentially the same as those in lasat mice, and hence the lasat mice could be saved.

Animals↗
PubMed1981

Ultrastructural features of large cell carcinoma of the lung with reference to the prognosis of patients.

Epon embedded sections of large cell carcinoma of the lung obtained from 26 patients were examined by light and electron microscopy. On an ultrastructural basis this carcinoma was subclassified into four types: squamous, adenosquamous, adenocarcinomatous, and giant cell types. The giant cell carcinoma was regarded as a special type of squamous or undifferentiated carcinoma. Transition between each of these types was clearly demonstrated. Follow-up study revealed that a close correlation existed between these types and the prognosis of the patients, the squamous type showing a better prognosis and the adenosquamous and adenocarcinomatous types exhibiting equally poor prognoses. The giant cell type invariably pursued a fulminant course. It was suggested that each of the types might represent different stages and directions of differentiation of cancer cells originating from the same ancestry. The authors' view favors the Y construct theory concerning the histogenetic pedigree of pulmonary carcinoma.

Adenocarcinoma↗
PubMed1981

Shoshin beriberi with severe metabolic acidosis.

A case of Shoshin beriberi found in a patient with severe metabolic acidosis and shock was presented. We believe the metabolic acidosis in this case is ascribable to overproduction of pyruvate and lactate resulting from lack of thiamine due to preferential intake of sake, precooked food, and rice, and also to shock. The hemodynamic studies done after recovery from shock revealed that the cardiac output, which was initially high, decreased to a slightly low level and then increased to a normal level, concomitant with an increase in the peripheral vascular resistance after introductory administration of thiamine. After treatment with thiamine, all abnormalities disappeared.

Acidosis↗
PubMed1981

Formation of mononuclear phagocyte (macrophage) colonies by mouse spleen cells in liquid culture. II. Its inhibition by the capsular polysaccharide of Klebsiella pneumoniae.

The effect of the capsular polysaccharide of Klebsiella pneumoniae type 1 Kasuya strain (CPS-K) on the formation of macrophage colonies in cultures of mouse spleen cells was investigated by the liquid culture technique during an incubation period of 7-8 days. CPS-K markedly inhibited further generation of macrophage colonies when added at any time after the beginning of culture, whereas it showed no destructive effect on macrophage colonies which were already formed before its addition. When CPS-K was present throughout the incubation period, such a low concentration as 0.05 micrograms/ml significantly inhibited colony formation, and the intensity of its inhibitory effect depended on its dose in the range of 0.005-50 micrograms/ml. The inhibitory effect persisted even if CPS-K was washed out after spleen cells were kept in contact with 20 micrograms of CPS-K per ml at 37 C for 6 hr. It was found that the inhibitory effect of CPS-K on colony formation was not mediated through its action on T cells, B cells or macrophages, and that it was not due to the generation of suppressor cells capable of inhibiting colony formation. It is concluded therefore that CPS-K directly inhibits the proliferation of macrophage colony-forming cells. The active substance responsible for the inhibitory effect of CPS-K on colony formation is the neutral polysaccharide fraction of CPS-K.

Animals↗
PubMed1981

Further Studies of the polysaccharide of Klebsiella pneumoniae possessing strong adjuvanticity. I. Production of the adjuvant polysaccharide by noncapsulated mutant.

In culture fluid, Klebsiella pneumoniae type 1 Kasuya strain produces polysaccharide exhibiting a strong adjuvant effect. The active substance responsible for the strong adjuvant effect of the polysaccharide isn ot its acidic polysaccharide fraction (the type-specific capsular antigen) but the neutral polysaccharide fraction. In the present study, a mutant which did not produce the type-specific capsular polysaccharide was isolated from ultraviolet-irradiated cells of K. pneumoniae type 1 Kasuya strain which had been labeled with leucine-requiring marker by selecting unagglutinable cells with the antiserum to he type-specific capsular polysaccharide. Serological tests showed that the type-specific acidic capsular polysaccharide was present neither on the cells surface nor in the culture fluid of the mutant. Electron microscopically, the mutant did not possess any capsular material. On the other hand, nearly an equal amount of neutral polysaccharide antigen as produced in culture fluids of the noncapsulated mutant polysaccharide antigen was produced in culture fluids of te noncapsulated mutant and the parent strain. The neutral polysaccharide antigen produced by the noncapsulated mutant exhibited the same degree of strong adjuvant effect on antibody response to bovine gammaglobulin in mice as that produced by the parent strain. The relationship between the neutral polysaccharide antigen in culture fluid and the O antigen of K. pneumoniae was discussed.

Adjuvants, Immunologic↗
PubMed1981

Further studies of the polysaccharide of Klebsiella pneumoniae possessing strong adjuvanticity. II. Serological relationship between the adjuvant polysaccharide and O3 antigen of Klebsiella.

The serological specificity of the neutral polysaccharide possessing extraordinarily strong adjuvanticity originally isolated from the culture supernatant of Klebsiella K1 strain Kasuya has been investigated. Among all of the reference strains (K1-K82) of Klebsiella obtained from the International Escherichia and Klebsiella Center, Statens Seruminstitut, Copenhagen, only 13 strains have been shown to produce the adjuvant polysaccharide by the passive hemagglutination inhibition test. All of these 13 strains belong to the O3 group, and the strains which belong to other O groups of which were not identifiable did not produce it. The gel precipitation test has demonstrated that the adjuvant polysaccharide is antigenically identical to O3 antigen isolated from the cells of the decapsulated mutant (strain LEN 1) of Klebsiella K1 strain Kasuya and to O9 antigen of Escherichia coli isolated from either the culture supernatant or the cells, which has already been shown to be antigenically and structurally identical to the O3 antigen of Klebsiella.

Adjuvants, Immunologic↗