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Biomedical subjects

M Lee

Publications and source records attributed to M Lee.

At least 1,063 records · Page 59Linked to original sources
PubMed1981

A rapid culture-harvest protocol for amniotic cell cultures.

A rapid culture-harvest technique for amniotic fluid is presented using the poly-L-lysine technique (Rajendra et al. 1980). The procedure is simple, reproducible, and involves harvesting primary cultures five to eight days after culture. This technique is particularly useful as an adjunct to routine procedures, in advanced gestational age where preliminary chromosome counts are required to relieve anxiety, sex determination when sex linked traits are involved, and in situations where the cultures produce very small colonies that do not proliferate or respond to sub-cultures and thus are difficult to harvest.

Amniotic Fluid↗
PubMed1981

Evaluation of low-viscosity intravascular silicone rubber with scanning electron microscope.

A low-viscosity silicone rubber polymer used as an intravascular embolization material was studied with the aid of a scanning electron microscope. The silicone polymer became permanently affixed to the vessel endothelium without observable morphologic changes in the endothelium. No evidence of late recanalization of the embolized vessels was found in our experimental sections.

Animals↗
PubMed1981

Effect of gibberellic Acid on silica content and distribution in sugarcane.

The effect of gibberellic acid on the content and distribution of silicon in the stem, leaf sheath, and leaf lamina of sugarcane was analyzed in relation to the effect of gibberellic acid on stem growth. Silicon content was measured by neutron activation analysis, and its distribution was followed by scanning electron microscopy and X-ray analysis.Foliarly applied gibberellic acid increased stem length and fresh weight and decreased silicon content. Gibberellic acid treatments had little or no effect on growth or silicon content of leaf laminae or sheaths. The close correlation between increase in growth of an internode in response to gibberellic acid and the decrease in silicon content of that internode indicated a dilution effect of growth on the amount of silicon rather than a direct effect of gibberellic acid on silicon deposition. This conclusion was supported by scanning electrom microscopy, X-ray map photos, and counts of silica cells per unit of epidermis area.

Journal Article↗
PubMed1981

Phenotypically normal individuals with an inversion (X) (p22q13) and the recombinant (X), dup q.

Two families are described in which there is an inv(X) (p22q13) which has been transmitted for three generations. In one family (K482), no recombinants have been recovered and the inversion can be traced to a female born in 1839. In the second family (K491), a recombinant (X), dup q, has been recovered in a normal fertile woman. In both families the inverted X appears to be carrying the Xg allele. Despite extensive family studies no common ancestor has been found for the two families. The pattern of DNA synthesis has been studied in those individuals who are karyotypically 46,X,inv(X) (p22q13) and 46,X,rec(X)dup 1, inv(X) (p22q13); the selection of the abnormal as the late synthesizing X chromosome is random in the former and total in the latter. In some cells the two long arms of the recombinant X chromosome showed asynchrony of DNA replication.

Adult↗
PubMed1981

Tartrazine-containing drugs.

Although the incidence of tartrazine sensitivity in the general population is low, serious adverse reactions have occurred. To prevent unnecessary exposure of sensitive patients, physicians should avoid prescribing tartrazine-containing drugs. Because of new FDA requirements that manufacturers list tartrazine dye in both over-the-counter and prescription drugs, many manufacturers have reformulated their products to remove this colorant. Therefore, previously published lists of tartrazine-containing drugs are outdated. We conducted a survey of American manufacturers to derive a current list of tartrazine-containing drugs. This list is intended for use as a guide for health professionals who wish to avoid products containing tartrazine in prescribing for patients sensitive to it.

Azo Compounds↗
PubMed1981

Long-term rehabilitation in advanced stage of childhood onset, rapidly progressive muscular dystrophy.

The most common cause of death in patients with rapidly progressive neuromuscular disease has been respiratory failure. The medical community and society as a whole have not yet recognized the possibilities for markedly extended life expectancy and prolonged productivity in severely disabled neuromuscular patients with proper medical management and rehabilitation. In a population of 120 patients with early childhood onset, rapidly progressive muscular dystrophy, 29 were in the most advanced stage requiring mechanical ventilation 24 hr/day and had vital capacities of less than 10% predicted for age and height. Of these patients with severely weakened bulbar innervated muscles, 10 have been receiving positive pressure ventilation via indwelling tracheostomy tubes for an average of 3 years. The average age of the 29 patients is 27 years with a range of 15 to 54 years. Of the 29 patients, 24 live in the community. Three are married and have a total of 5 children. Some patients are in professional careers, or active in political and community organizations. The management of and potential for improved quality of life and increased longevity in these patients are discussed.

Adolescent↗
PubMed1981

The protective effect of a cold weather mask on exercised-induced asthma.

The authors investigated the usefulness of a cold weather mask in reducing exercise-induced bronchospasm. Ten asthmatic subjects were selected because of previously demonstrated exercise-induced bronchospasm. Airway responses to submaximal exercise were measured on four separate days. Exercise was performed on the first two days at ambient temperature in a centrally air-conditioned laboratory and on the second two days in a refrigerated room. Exercise challenge was similar on each of the two days of the separate environmental conditions, with the exception that a mask was worn on one of the two days. Pulmonary function was measured using maximal and partial expiratory flow volume curves before and after exercise. On both of the days during which subjects exercised without a mask, significant decreases in all pulmonary function parameters occurred. Protection was seen under both conditions, but was more complete in the cold. These studies demonstrate the therapeutic potential of cold weather masks in protecting asthmatics against the effects of exercise and cold air.

Adolescent↗
PubMed1980

A new and simple technique for chromosomal preparations from peripheral blood lymphocytes, amniotic cell cultures, skin fibroblasts, bone marrow and single cell clones when the yields from harvests are low.

A technique for chromosomal preparations from low yields harvest is presented using Poly-L-Lysine (300,000-400,000 MW). The procedure can be successfully used for peripheral blood lymphocytes, amniotic cell cultures, skin fibroblasts, direct preparations from bone marrow, cloned clusters and colonies from bone marrow grown on agar or methyl cellulose and eggs and blastocysts, with applications to other tissues. The technique is simple with minimal cell loss, and chromosomal preparations of good quality can be obtained which are amenable to all banding techniques.

Amniotic Fluid↗
PubMed1980

Effect of pH on the Activity of Some Respiratory Inhibitors.

Inhibition of respiration of cultured cells of Petunia hybrida by NaF, NaN(3), malonic acid, and salicylhydroxamic acid increased at low pH. This increase could be partially reversed by raising the pH of the medium. Uptake experiments show that the greater inhibition of malonic acid at low pH was not the result of greater uptake. The results suggest that the increase in inhibition at low pH could not be attributed to greater cell penetration.

Journal Article↗
PubMed1980

Compensatory adrenal hypertrophic response in fetuses of calorie-malnourished rats.

Fetuses of ad libitum fed and calorie-restricted rats were unilaterally adrenalectomized on day 19. By day 20 ad libitum fed fetuses exhibited compensatory adrenal hypertrophy, but calorie-restricted fetuses did not. As this may be due to the elevated corticosteroid levels of the calorie-restricted dams, unilateral adrenalectomy was performed on fetuses of control and restricted rats bilaterally adrenalectomized on day 15. Fetuses of restricted and control rats exhibited compensatory hypertrophy. One effect of calorie restriction may be to increase the amount of maternal corticosteroids acting on the fetus.

Adrenal Cortex↗
PubMed1980

Lymphomatoid granulomatosis. Report of a patient with severe anemia and clubbing.

We report a 31-year-old man in whom initially localized lymphomatoid granulomatosis was diagnosed at left pneumonectomy. He had severe anemia which is rare and clubbing of the fingers and toes, a feature not previously described. Five months following surgery, disease spread to the right lung, and atypical lymphomatous transformation occurred in cervical and mediastinal lymph nodes, leading to a superior vena cava syndrome. Chemotherapy with cyclophosphamide and prednisone resulted in significant resolution initially, but ultimately, the patient had progressive axillary node enlargement and succumbed. Clinical and roentgenographic improvement and later deterioration following pneumonectomy and improvement with chemotherapy were mirrored by changes in degree of clubbing, leukocytosis, and elevation of erythrocyte sedimentation rate.

Adult↗