Biomedical subjects
Publications and source records attributed to M Krutsay.
A modified method is described based on the following steps: 1. simultaneous mordanting, using an alcoholic solution of phosphotungstic acid and tannic acid, 2. treatment with pH = 10.4 silver nitrate solution, 3. physical development (one or two phases).
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A method is described, which is based on mordantiation with a concentrated silver nitrate solution at 60 degrees C followed by two-step physical development at room temperature, with formalin as the reducing agent.
All types of amyloid can be quickly and selectively demonstrated by staining with an alcohol-containing Sirius red solution.
A method is described, which is based on an impregnation with a silver nitrate solution (pH = 5,0), and on a physical development.
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A method based on mordantation with silver nitrate solution at pH = 3.0 and on physical development is described.
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With the hydrochloric acid-alcoholic solution of alcian green, without any pretreatment both the sulfated and the carboxylated proteoglycans can be demonstrated. After an indirect sulfation (periodic acid-bisulfite pretreatment), the glycogen and the glycoproteins, and after an oxidation with acidified potassium permanganate, the high cystine-containing proteins can also be stained. As a counterstain, a picric acid-Sirius red solution has proved good.
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To demonstrate carbohydrate-containing tissue elements becoming argyrophil by treatment with chromic acid and periodic acid respectively, the author proposes instead of the methenamine-silver nitrate solution the successive application of a silver nitrate solution, adjustable to the optimal pH, and of a physical developer.
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A method is described based on mordantiation with a sodium carbonate solution and a physical developing technique.
The author describes a method, which is based on mordantings with phosphotungstic acid and tannic acid as well as on an impregnation with ammoniacal silver nitrate solution and on a reduction in formalin.
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A method for silver impregnation of connective tissue is proposed which utilizes a physical developer instead of the traditional approach using successive treatments with ammoniacal silver solution and formalin.