On the resorcin-fuchsin stainings. III. Demonstration of sulfated glycoproteins and proteoglycans.
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Clusterin↗
Biomedical subjects
M Krutsay
Publications and source records attributed to M Krutsay.
[Vasoactive polypeptide-producing pancreatic carcinoma].
Authors report a case of Verner-Morrison syndrome which occurred in a 75-year-old man. The syndrome was caused by a pancreas VIP-oma with the histological structure of adenocarcinoma. Treatment with somatostatin analogous (octreotid) was effective, but the outcome was lethal due to subsequent pulmonary embolism.
Adenocarcinoma↗
[Neuromuscular atrophy].
Author reports a case of neural muscular atrophy of a 59-year-old female patient, verified by autopsy and histologic examination. The histologic picture corresponded to the both I. and III. type of sensory-motor polyneuropathyes.
Age Factors↗
[Pathological diagnosis of polyganglioradiculitis in apropos of a case].
The case of a 60-year-old woman is reported, who died from polyganglioradiculitis and pulmonary embolism. The authors emphasize the importance of the suitable autopsy technic for the exploration of the spinal ganglia.
Autopsy↗
[A new method for simultaneous demonstration of senile plaques and Alzheimer's neurofibrillary tangles].
The author completed the method according to Yamaguchi with a sodium hydroxide pretreatment, and he also modified the composition of the methenamine-silver nitrate solution. The Alzheimer cells as well as the classic (combined, neuritic) senile plaques and the diffuse plaques can be impregnated simultaneously with the new procedure.
Aged↗
[Rapid course amyotrophic lateral sclerosis].
The case of a 58-year-old woman is reported, whose illness began with a pain in the left shoulder. Later a spastic palsy developed in the hands, and a flaccid one in the arms and lower limbs. After a treatment for 12 months she died from respiratory failure. The motor nerve cells in the spinal cord, brainstem and cerebral cortex together with the pyramidal tracts had degenerated symmetrically.
Amyotrophic Lateral Sclerosis↗
[Polyethylene glycol as solvent for lipid stains].
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Humans↗
[Histochemical detection of copper. II. The dithizone method].
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Copper↗
[The role of permeability in histological staining].
Ionic stain-binding is influenced by number of acid and basic groups, their dissociation equilibrium coefficient and isoelectric point of proteins, respectively, and by permeability, on tissue side and acid and basic character, respectively, ionic volume, isochronic and succedaneous way of application, respectively, and hydrogene ion concentration of solution on side of stain. With joint or sequent use of acid stains of different ionic volume, permeability order of certain tissue elements, their relative permeability can be determined.
Histological Techniques↗
[The impregnation of glial fibers].
The author studied the influence of the solvents and of the rinsing fluids on the result of silver impregnation. A titrimetric method is described for the determination of the concentration of formaldehyde solutions.
Formaldehyde↗
[Differential staining of calcium compounds in histologic sections].
An experimental protocol is proposed for the differentiation of calcium-hydrogen-phosphate, tri-calcium-phosphate, calcium-oxalate, calcium-urate and fatty acidic calcium. For tissue fixation calcium acetate-formaldehyde is recommended in which fatty acids and earth-metal urates form insoluble calcium-salts which can be demonstrated in paraffin sections.
Animals↗
[Silver impregnation of the glomerular basement membranes].
A method based on the periodic acid-produced (secondary) argentaffinity of the basement membranes is described. Sodium tetraborate is used for alkalizing of the silver nitrate solution.
Arteries↗
[Silver impregnation of microglial cells and astrocytes in paraffin sections].
A method based on mordanting with sulphuric acid-potassium ferrocyanide solution and on physical development is described.
Animals↗
[Fixation of tissue specimens. II].
As a routine fixative calcium-acetate-formol is recommended causing no tissue shrinkage, haemolysis, formaldehyde pigments and having an almost neutral pH. Due to the formation of insoluble calcium salts it preserves fatty acids and water-soluble urates and makes them withstanding to paraffin embedding. If completed with methylene blue this solution is also suitable for an improved fixation of acidic glycosaminoglycans.
Acetates↗
[Re-coloration of pathological preparations exhibited in museums].
Author recommends a formaldehyde solution containing sodium ditionite, piperazine and sodium acetate to re-colour organs stored in sodium acetate-formaldehyde. The hermetic closure of the solution is achieved by layering paraffin oil over the fixative.
Acetates↗
[Staining of hyaline substances].
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Amyloid↗
[Silver impregnation of connective tissue. V].
A method is described on mordantiation with an alcoholic solution of tannic acid and phosphotungstic acid followed by a two-step physical development.
Connective Tissue↗
[Amyloid staining. III].
Anisotrophy of collagen fibres can be abolished, without change of the anomalous colour of amyloid, by mounting Congo-red stained sections in glycerin. Treatment with sodium hydroxide solution prevents staining of secondary amyloid with Sirius red.
Amyloid↗