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Biomedical subjects

M Kondo

Publications and source records attributed to M Kondo.

At least 1,351 records · Page 75Linked to original sources
PubMed1981

Studies on protease from marine bacteria. 1 Isolation of marine Pseudomonas sp 145-2 and purification of protease.

Marine Pseudomonas strain 145-2 having the ability to produce extracellular protease was isolated from Uwa sea water, Ehime prefecture in Japan. This strain grows at 0 degrees - 37 degrees C, but protease production was observed only during 6-10 h cultivation at 20 degrees - 30 degrees C, when casein, as the nitrogen and carbon source in a concentration of 0.5-1.0%, was used. Maximum production of protease by the strain was also observed. Na, K, Ca and Mg ions were needed for growth and production of protease. Two extracellular proteases (proteases I and II) were purified from the culture supernatant by ammonium sulphate fractionation following Sephadex gel chromatography. Protease I had a larger molecular weight than protease II. After purification, proteases I and II were revealed as homogeneous by acrylamide gel electrophoresis and immunoelectrophoresis.

Amino Acids↗
PubMed1981

Treatment of ulcerative colitis by the direct administration of an antifibrinolytic agent as an enema.

Fibrinolytic activity in biopsied colonic mucosa was examined in patients with ulcerative colitis, and most cases were found to have increased tissue fibrinolysis -- due mainly to tissue plasminogen activator -- in the affected mucosa. Five cases, 4 with elevated tissue fibrinolysis and 1 normal, were treated with an antifibrinolytic agent, tranexamic acid (trans-AMCHA) administered as an enema, to inhibit fibrinolysis of the affected mucosa directly. In patients with elevated mucosal fibrinolysis, 2 showed complete remission after tranexamic acid enema alone, and there was one remission in response to combination treatment with oral prednisolone. One case with slightly elevated mucosal fibrinolysis showed clinical improvement, although the radiological findings were unchanged. No response was observed in one case with normal tissue fibrinolysis. It is concluded that tranexamic acid may show a therapeutic effect in ulcerative colitis with elevated mucosal fibrinolysis when administered via an enema, which allows direct contact of the drug with the affected mucosa.

Adolescent↗
PubMed1981

Diagnosis of pancreatic cancer by pancreatic oncofetal antigen (poa) in pure pancreatic juice.

Pancreatic oncofetal antigen (POA) was detected in the pure pancreatic juice by double immunodiffusion assay in a series of patients, using anti-POA prepared by immunizing rabbits with human fetal pancreas homogenate. The test was positive in as many as 72% of the patients with pancreatic cancer studied, whereas only less than 10% of patients with other diseases or normal controls were positive for this antigen, thus suggesting a potential usefulness of the pure pancreatic juice assay for POA in the diagnosis of cancer of the pancreas. The POA has proven to be distinct from such oncofetal antigens as AFP and CEA, to be labile to heating at 85 degrees C, to show beta-mobility on immunoelectrophoresis and to have a molecular weight of approximately 37,000 as estimated by gel filtration chromatography.

Adult↗
PubMed1981

Protective effect of levamisole on carbon tetrachloride-induced lipid peroxidation and hepatotoxicity.

It has been suggested that lipid peroxidation is an important factor in the pathogenesis of carbon tetrachloride (CCl4) hepatotoxicity. In the present study, experimental liver injury induced by CCl4 could be prevented by levamisole, an antihelminthic agent and immunomodulator. Despite of exposure to CCl4 tissue levels of thiobarbituric acid (TBA) reacting substances in the liver were not increased in rats pretreated with levamisole. On the other hand, when we administered levamisole, 150 mg p. o. daily given on three consecutive days of each week, to six elderly patients with arteriosclerosis. They all showed a significant decrease in serum TBA reactive substances during the 12 weeks of therapy. This provides evidence that levamisole prevents liver damage by CCl4, and indicate the possibility that levamisole might have an antioxidative effect or may prevent the accumulation of TBA reactive substances.

Aged↗
PubMed1980

Lipovitellin from the crustacean, artemia salina. Biochemical analysis of lipovitellin complex from the yolk granules.

The polypeptide, lipid, and carbohydrate components of the lipovitellin complex from anostracan crustacean, Artemia salina, have been investigated. The lipovitellin isolated from the yolk granules is a carotenoid.lipoglycoprotein complex, containing 3.3% carbohydrate and 8.6% lipid. About half of the carbohydrate fraction is mannose and the rest consists of galactosamine, galactose, and glucosamine. The lipid fraction contained 67% phospholipid and 33% neutral lipid. Phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine are three major phospholipid components and their relative ratio changes by developmental stages. The neutral lipid fraction is composed of mainly triacylglycerol and cholesterol. Two polypeptide chains are identified as apoprotein components of the lipovitellin complex. Their molecular weights are estimated by sodium dodecyl sulfate gel electrophoresis to be 190,000 and 68,000, respectively. The third polypeptide with Mr = 85,000 is a very minor component and exists in nonstoichiometric levels in the lipovitellin complex. On the basis of an apparent molecular weight of the lipovitellin complex determined by gel filtration chromatography, the dimeric lipovitellin form is suggested, each monomer of which contains one set of Mr = 190,000 and one set of Mr = 68,000 apoproteins. Each dimeric lipovitellin complex contains approximately 70 associated lipid molecules, of which about 4 are attributed to canthaxantin, and 100 bound carbohydrate molecules.

Amino Acids↗
PubMed1980

Affinity chromatography of poly(adenylic acid)-containing ribonucleoproteins on oligothymidylic acid-cellulose.

Binding of poly(A)-containing RNP to oligo(dT)-cellulose has been investigated as a function of mono- and divalent ion concentration. 80-90% binding was obtained either in high (500 mM) or in moderate NaCl concentrations in the presence of 5 mM MgCl2. At 40 mM NaCl and 5 mM MgCl2 poly(A)+-RNP exhibit approximately the same stability as poly(A)+-RNA in binding to oligo(dT)-cellulose with a melting temperature of 41 and 45 degrees C, respectively, indicating that the protein moiety has no effect on the ribonucleoprotein binding in these conditions. Differences were observed in the elution of poly(A)+-RNA and poly(A)+-RNP from oligo(dT)-cellulose in buffer without salts. Poly(A)+-RNA was completely removed at 4 degrees C whereas the melting temperature of poly(A)+-RNP was only decreased to 34 degrees C. The isolation of poly(A)+-RNP by thermal elution from oligo(dT)-cellulose is described.

Animals↗
PubMed1980

Carbohydrate alters the trypanocidal activity of normal human serum with Trypanosoma brucei.

Three categories of carbohydrates are distinguished based on their effects on the trypanocidal activity of normal human serum on Trypanosoma brucei. The first category includes carbohydrates such as glucose, mannose and fructose which are utilized by parasites as an energy source. These carbohydrates inhibit the trypanocidal activity in a concentration-dependent manner. The third category, represented by xylose and mannitol, are not energy sources to the parasites but inhibit the trypanocidal activity in a manner similar to the first category carbohydrate. However, at very high concentrations (approximately 140-150 mM), the inhibition is reversed. Glycerol is the only known carbohydrate belonging to the second category. Glycerol enhances the trypanocidal activity of normal human serum and of those sera which normally display no cytotoxic activity, although glycerol serves as an excellent energy source. On the basis of these results, the mechanisms by which these three categories of carbohydrate could alter the trypanocidal activity are discussed in terms of the interaction between a trypanocidal factor and the surface receptor.

Animals↗
PubMed1980

Studies on the bacterial spore coat. (7) Properties of alkali-soluble components from spore coat of Bacillus megaterium.

Properties of alkali-soluble components from the spore coat of Bacillus megaterium were examined by physicochemical methods. They were composed of acidic polypeptides of various molecular weights with small amounts of phosphorus and sugar. They were allowed to dissociate to unit components by incubation with SDS. The major component was partially purified by gel filtration, and shown to have a mean molecular weight of about 11,000.

Bacillus megaterium↗
PubMed1980

Location of elements in ashed spores of Bacillus megaterium.

The structure of the skeleton of spores of Bacillus megaterium was examined after ashing in a plasma asher and the elemental composition of the ashed whole spores was determined with an analytical electron microscope. All spores were ashed in situ although they shrank by about 15%. Even P and S, in addition to metals, were recovered well from ashed samples. Ash was rich in the core and the coat, and poor in the cortex. Ca, P, S, and Mg were detected in the core and coat of the spore of B. megaterium QM B1551. Ca in the core was markedly decreased by germination or autoclaving. In the spore of B. megaterium ATCC 19213, almost all of the ash was detected in the core and its elemental composition was similar to that of the core of the strain QM B1551 spore. These reuslts suggest strongly that the core is the site of Ca associated with dipicolinic acid.

Bacillus megaterium↗