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Biomedical subjects

M Kitagawa

Publications and source records attributed to M Kitagawa.

At least 469 records · Page 26Linked to original sources
PubMed1989

[A case of spermatocytic seminoma].

In April, 1987, we treated a 33-year-old male patient who presented a painless swelling of the right scrotal content of 6 months duration. After surgery, the resected testis weighed 130 g, and the cut surface showed an edematous, white-yellow, homogenous tumor. A histopathological examination revealed a typical spermatocytic seminoma composed of three types of tumor cells. The small cells had pyknotic nuclei and an eosinophilic cytoplasm while the medium-sized cells and most of the giant cells had nuclei containing granular chromatins. Some of giant cells contained a filamentous, chromatin-like spireme of primary spermatocytes. A chest X-ray, CT scanning and lymphography revealed no metastatic lesions, and the patient is still alive with no evidence of disease 10 months after the orchiectomy.

Adult↗
PubMed1988

Distribution of tocopherol among human plasma lipoproteins.

A study of the distribution of alpha-tocopherol among the plasma lipoproteins of 19 healthy adults, 20 pregnant women at delivery, and the umbilical cord blood of their fetuses has shown the following. 1. In healthy adults, LDL in males contained slightly more tocopherol than did HDL, while non-pregnant females showed the opposite tendency. 2. In the pregnant women, the percent distribution of tocopherol was much higher in VLDL fractions, and lower in HDL fractions, as compared with non-pregnant women. 3. In cord blood, the percent distribution of tocopherol was extremely low in LDL fractions, but it was high in HDL fractions. 4. The distribution of tocopherol among lipoproteins was directly related to the total lipid contents of the lipoprotein fractions in all groups examined. After the oral administration of tocopherol to healthy adults, the changes in RBC tocopherol concentrations correlated most closely with those in HDL tocopherol.

Adult↗
PubMed1988

Sensitive serum markers for detecting pancreatic cancer.

Serum amylase, immunoreactive elastase (IRE), carcinoembryonic antigen (CEA), and carbohydrate antigen (CA 19-9) were measured in 40 patients with pancreatic cancer. IRE and CA 19-9 were further assayed in nonpancreatic malignancies (n = 98) and chronic nonmalignant disease (n = 194). In pancreatic cancer, elevated values were observed for amylase in 30% of the patients, for IRE in 70%, for CEA in 28%, and for CA 19-9 in 68%. Elevation of IRE and/or CA 19-9 was found in 95% of the 40 patients. Elevated serum IRE was observed more frequently in head cancer and resectable cancer, whereas elevation in CA 19-9 occurred more often in body-tail cancer and unresectable cancer. Elevation of serum IRE tends to occur at earlier stages of pancreatic cancer. No one test is adequate for the accurate diagnosis of pancreatic cancer. However, the two assays complement each other, and their combined use would provide a sensitive clue for tentative diagnosis of pancreatic cancer.

Adenocarcinoma↗
PubMed1988

Expression of the insulinoma gene rig during liver regeneration and in primary cultured hepatocytes.

We have isolated a novel gene, rig (rat insulinoma gene) from rat insulinomas. In the present study, rig was found to be expressed in rat regenerating liver and in primary cultured rat hepatocytes. The level of rig mRNA was increased at the proliferative phase of liver regeneration. In synchronously cultured hepatocytes, the rig mRNA level was elevated at the G1 phase of the cell cycle and the rig-protein was accumulated in the nuclei during the S phase. These results indicated that rig could be involved in a more general way in growth or cell replication.

Adenoma, Islet Cell↗
PubMed1988

Inhibition of RNA synthesis in embryo of Xenopus laevis by protease inhibitor.

We have studied the role of proteases during the development of Xenopus laevis embryos with the aid of protease inhibitors. The activity of proteases was found to be only minimal in the unfertilized egg and during the initiation of development, but activity began to increase at the morula stage. When the activity of proteases was inhibited by antipain, an inhibitor of endopeptidase activity, RNA synthesis in the embryo was inhibited. To examine the relationship between the inhibitory effect of antipain on protease activity and its effect on RNA synthesis, antipain was reduced with NaBH4 to inactivate its protease inhibitory activity. The reduced antipain did not inhibit RNA synthesis in the embryo. Antipain effectively inhibited synthesis of both rRNA and poly(A)+RNA but not 4S RNA. We therefore suggest that protease activity plays an important role in the initiation and/or continuation of RNA synthesis.

Animals↗
PubMed1988

Pancreatic and biliary excretion of camostat in dogs.

It has been reported that camostat (Foy-305), a synthetic proteinase inhibitor, is excreted into the biliary-pancreatic juice in rats. To confirm whether camostat is excreted into bile or pancreatic juice, camostat (5 mg/kg/h) was given intravenously to dogs with pancreatic or biliary fistulae. Camostat and its active metabolite (Foy-251) in bile and pancreatic juice were measured by high-performance liquid chromatography (HPLC) and by their trypsin-inhibitory activity. Camostat did not alter the exocrine pancreatic secretion of fluid, bicarbonate, protein or trypsin stimulated by secretin (1 Crick-Harper-Raper unit/kg/h) and cerulein (50 ng/kg/h). Camostat and its active metabolite were detected in bile by HPLC and trypsin-inhibitory activity (the mean excretion rate was 1.3% of the intravenous dose of camostat), but they were not detectable in pancreatic juice. In conclusion, camostat and its active metabolite were excreted into bile but not into pancreatic juice.

Animals↗
PubMed1988

[Rapid diagnosis of experimental tumor by FITC-Con A lectin--a comparative study of smear and section specimens].

FITC-Con A staining as a rapid diagnostic method for tumor cells was applied to the tumors smeared on glass slide and section specimens to evaluate the reactivity with FITC-Con A. Good staining results were obtained in smear specimens with clear fluorescence on the membrane of tumor cells. Con A and LCH lectins bound well with tumor cells to produce strong fluorescence in comparison with PEA and DBA. It indicates that tumor cells expressed dominantly the receptors of alpha-D-glucose and alpha-D-mannose sugar chain on the membrane of tumor cells. From these results it was concluded that FITC-Con A staining method applied to smear specimens is more advantageous in the rapidity and the simplicity for tumor cell diagnosis than section specimen method.

Animals↗
PubMed1988

[Cerebral infarction and subarachnoid hemorrhage caused by dolichoectasia; report of a case].

Dolichoectasia usually causes neurological symptoms because of the pressure it exerts on adjacent intracranial structures and/or impaired circulation distal to ectasia. However, it rarely induces subarachnoid hemorrhage. The authors report just such a rare case of dolichoectasia, which caused subarachnoid hemorrhage following extensive cerebral infarction distal to ectasia. On October 18, 1982, a 47-year-old man came to our hospital with dementia which had started 7 days before, as his chief complaint. Neurological examinations revealed no positive findings. However, plain CT scan showed the presence of small multiple low density areas with a round-shaped high density area at the tip of the right temporal lobe. Angiogram taken on February 18, 1983, revealed that all main cerebral arteries, especially the C1 portion of the right internal carotid artery, and the M1 portion of the right middle cerebral artery were abnormally tortuous and distended. There was also pooling of contrast medium in the distal part of the M1 portion of the right middle cerebral artery which corresponded to the site of high density area on the plain CT scan. On January 31, 1987, he was admitted to our hospital with his chief complaint being the sudden onset of weakness in his left extremities. On admission, he was confused (II-10 on the Japan Coma Scale), dysarthric, and hemiparetic on the left side. Plain CT scan showed extensive cerebral infarction of the right middle cerebral artery territory distal to the ectasia.(ABSTRACT TRUNCATED AT 250 WORDS)

Cerebral Angiography↗
PubMed1988

Determination of alpha-tocopherol in buccal mucosal cells using an electrochemical detector.

Using HPLC with an electrochemical detector, tocopherol concentration in human buccal mucosal cells was determined, and compared with that in plasma, RBCs, and platelets. The values were expressed on the basis of protein concentration. The reproducibility and recovery were enough to be used clinically. In healthy young male adults, tocopherol concentration in buccal cells was 46.4 ng/mg protein, and this increased three fold, after daily administration of 600 mg of d-alpha-tocopherol for one month. Comparison of correlations between all the measurements showed that the highest correlations were between RBC tocopherol level and the tocopherol/lipid ratio in plasma, and between platelet tocopherol level and the tocopherol/lipid ratio. Tocopherol concentration in buccal cells correlated at r = 0.66 (p less than 0.03) with RBC tocopherol, r = 0.65 (p less than 0.03) with the tocopherol/lipid ratio, and r = 0.60 with platelet tocopherol concentration, respectively.

Adult↗
PubMed1988

[Accessory nerve paralysis due to compression of the fenestrated vertebral artery].

Neurological involvement due to fenestrated vertebral artery has not yet been reported, although fenestration formation by vertebral arteries is not rare. Reported here is one such rare case. In this case accessory nerve paralysis developed due to fenestrated vertebral artery. A 51-year-old woman was admitted to our department with tilting of the head to the right side as her chief complaint. This had gradually developed over a period of approximately one year. Neurological examination on admission showed the presence of marked atrophy of the left sternocleidomastoid muscle with hyperactive deep tendon reflexes in her four limbs. Metrizamide CT scan showed compression of the upper cervical cord at the left side, and an abnormal small high density area was found at the same site on contrast enhanced CT scan. Left vertebral angiogram showed fenestration formation of the left vertebral artery at C1 level. Surgical decompression for the left accessory nerve and high spinal cord was planned because of the occurrence of symptoms due to compression of the fenestrated vertebral artery. C1 and C2 laminectomy was performed and the dura was opened in prone position. The fenestrated vertebral artery of the intraspinal portion was confirmed to be compressing the spinal root of the accessory nerve and high cervical cord. Initially, trapping of the fenestrated vertebral artery was attempted in the hope of sufficiently relieving compression on the accessory nerve and high cervical cord. This attempt resulted in failure, since auditory evoked response became definitely abnormal within two minutes following interruption of this abnormal artery. Because of this, insertion of Ivaron sponges between the abnormal artery and the accessory nerve was all that could be done.(ABSTRACT TRUNCATED AT 250 WORDS)

Accessory Nerve↗
PubMed1988

Urinary tract infections in patients undergoing transurethral prostatectomy.

The incidence of urinary tract infections was determined in 1,660 patients undergoing transurethral prostatectomy. Preoperative infection was found in 25% of all patients and in 35% of the 276 patients aged 80 years or over. Of the 1,251 patients without a preoperative infection, 17% had postoperative infections. The incidence of new postoperative infection was significantly related to the duration of surgery and the weight of the resected prostate. The incidence of infection was higher in summer than in winter months.

Aged↗
PubMed1988

Epididymitis after transurethral prostatectomy.

Transurethral prostatectomy was performed in 2,407 patients and open subcapsular prostatectomy in 308 patients. Postsurgical epididymitis developed in 1.0% of the patients undergoing transurethral resection and in 3.2% of the patients undergoing open resection (P less than 0.05). Factors associated with the incidence of epididymitis, such as prostate weight and operating time, were also associated with the incidence of urinary tract infection. Epididymitis was significantly associated with the presence of preoperative catheterization and postoperative urinary tract infection.

Aged↗
PubMed1988

Comparison of transurethral and open subcapsular prostatectomy: relation to weight of the prostate.

The results of transurethral prostatectomy in 2,269 men and open subcapsular prostatectomy in 300 men were compared. The only significant differences between the two groups were in the duration of hospital stay (18.3 vs 26.2 days) and the number of days that the drainage catheter was indwelling (5.4 days in the transurethral and 10.9 days in the open surgery group [P less than 0.01, both comparisons]). Blood loss, the need for transfusion, the amount of blood transfused, and the duration of surgery, catheterization, and hospital stay were all related to prostate size. Transurethral resection was superior to open resection for prostates weighing less than 20 gm; prostates of 20 gm to 30 gm were the easiest to enucleate.

Aged↗