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Biomedical subjects

M Kawashima

Publications and source records attributed to M Kawashima.

At least 379 records · Page 21Linked to original sources
PubMed1981

Two-site immunoenzymometric assay for thyrotropin in dried blood samples on filter paper.

We describe a sensitive, simple, and rapid two-site immunoenzymometric assay for thyrotropin in dried blood samples on filter paper, for use in screening for neonatal primary hypothyroidism. In this method, two dried-blood spots of 3 mm diameter (equivalent to about 5.4 microliter of blood) are incubated overnight with anti-thyrotropin-beta-D-galactosidase complex in an anti-thyrotropin coated tube. Then the enzyme activity in the washed tube is determined fluorophotometrically. The range of thyrotropin measurable is 10 to 160 milli-int. units/L blood. Values for thyrotropin in dried blood samples determined by this method and those determined by radioimmunoassay correlated highly (r = 0.96).

Blood Specimen Collection↗
PubMed1981

[Effects of cis-platinum diammine dichloride on yolk sac tumor transplanted in nude mice (author's transl)].

Effects of new anticancer drug Cis-platinum dichloride (CPDD) on yolk sac tumor were studied. Human yolk sac tumor serially transplanted in BALB/c nude mice (YST-1) was used as material in this experiment. Two administration methods of CPDD were examined and compared. 1) CPDD 5 mg/kg X 1 day X 4 2) CPDD i mg/kg X 5 days X 4. Tumor size was measured and serum AFP of nude mice was determined by radioimmunoassay method during experiments. At the end of experiments, treated tumor was resected and examined by light and electron microscopy. Results as follows: 1. CPDD was active in regressing yolk sac tumor at doses of 5 mg/kg given as a single treatment and doses of 1 mg/kg given daily for five days. It appeared that CPDD showed higher activity against tumors by daily injections at doses of 1 mg/kg. 2. Close correlation was recognized between tumor size and serum AFP of nude mice ((r = 0.93, p less than 0.001). Serum AFP of tumor-bearing nude mice revealed to be a useful marker of yolk sac tumor also in nude mice as in human patient. 3. Effect of CPDD on tumor cells was confirmed by light and electron microscopy.

Animals↗
PubMed1980

Fluctuation of the plasma TRH level in normal subjects in a 4-hour observation period.

Radioimmunoassayable TRH and TSH were measured in plasma samples taken at 5 min intervals for 4 hr (2100-0200 hr) from 4 normal male subjects. Three subjects showed a TSH surge at 2135 hr, 2455 hr and 0150 hr, respectively. The mean plasma TRH level of the 4 subjects was 10.3-11.7 pg/ml. Plasma TRH showed random fluctuation, which did not coincide with the nocturnal increase in plasma TSH.

Adult↗
PubMed1980

Enzyme immunoassay of thyroxine in serum and dried blood samples on filter paper.

A double antibody heterologous enzyme immunoassay was developed for measuring thyroxine (T4) in serum and in dried blood samples on filter paper for use in screening for neonatal hypothyroidism. In this method, ethanol extracts of 100 microliter of serum or dried blood spots of 9 mm in diameter were incubated with T4-alkaline phosphatase conjugate prepared with glutaraldehyde, and antiserum to T4-bovine serum albumin conjugate prepared with carbodiimide. The enzyme activity in the precipitates formed with second antibody was then determined photometrically at 500 nm. The measurable range of T4 was 1-32 micrograms/dl. A linear relation was obtained between the sample volume and the T4 value, and a high correlation was found between values for T4 determined by this method and those for T4 in serum determined by radioimmunoassay (coefficient of correlation: r=0.97 for serum and r=0.88 for dried blood samples).

Adult↗
PubMed1980

Relationship between the changes in cytoplasmic progesterone receptor concentration and in nuclear progesterone binding sites in the hen hypothalamus and pituitary during the ovulatory cycle.

The amount of specific [3H]-progesterone bindings was measured in cytosols and in nuclear fractions of the hen hypothalamus and pituitary during the ovulatory cycle. In both tissues, a decrease in cytosol binding from the peak at 18 and 8 hr before ovulation was coincident with an increase in nuclear binding attained a peak 14 to 6 hr before ovulation. The results suggest that progesterone may act on the hypothalamus and pituitary 18-14 hr before ovulation and 8-6 hr before ovulation.

Animals↗