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Biomedical subjects

M Ichikawa

Publications and source records attributed to M Ichikawa.

At least 379 records · Page 21Linked to original sources
PubMed1980

The ultrastructure of rapid-frozen, substitution fixed parotid gland acinar cells of the mongolian gerbil (Meriones meridianus).

The ultrastructure of gerbil parotid gland acinar cells studied by rapid freezing in liquid nitrogen and freeze-substituting with OsO4 was similar in general to conventionally prepared tissue, but different in several aspects. Acceptable preservation was limited to about 10 microgram of the surface; the secretory granule membrane was a single dense line, while all others were trilaminar; the dense-cored secretory granule had a rim of low density which was homogeneous; there were many continuous profiles between the Golgi lamellae, vesicles, and vacuoles; and mitochondria were long and arborized.

Animals↗
PubMed1980

Effect of treatment with 1-beta-D-arabinofuranosylthymine of experimental encephalitis induced by herpes simplex virus in mice.

1-beta-D-Arabinofuranosylthymine (ara-T) was examined for its therapeutic efficacy against encephalitis in mice inoculated intracerebrally with herpes simplex virus. Intraperitoneal treatment with 100 mg of ara-T per kg twice daily for 4.5 days was as effective as treatment with 50 mg of arabinosyladenine 5'-monophosphate per kg. Under the same conditions, doses of 5-iododeoxyuridine or arabinosylcytosine (50 mg/kg each) were not effective. Even when the virus inoculum was as high as 320 or 3,200 50% lethal doses, ara-T increased the life span significantly. Oral treatment with 27 mg of ara-T per kg produced a modest increase in the mean survival time, equal to that of 50 mg of ara-T per kg administered intraperitoneally or subcutaneously. A single dose of ara-T, 800 mg/kg intraperitoneally or 400 mg/kg orally, was effective. The 50% lethal dose of ara-T administered intraperitoneally and that administered orally were more than 10 and 15 g/kg, respectively. The therapeutic indexes (maximal tolerated dose divided by minimal effective dose) in multiple intraperitoneal treatments and in multiple oral treatments were estimated to be more than 25 and 100, respectively.

Animals↗
PubMed1979

RNase levels in golden hamster with DHPN induced pancreatic cancer.

Twenty Syrian golden hamsters recieved weekly injections of pancreatic cancer inducing DHPN. Their Poly (U) specific serum RNase levels were significantly elevated when compared to the control levels. Following salt fractionation, Poly (U) specific activity was present in both the 40% and 50% salt saturated fractions. Tissue assays showed that Poly (U) specific RNase was present in both pancreas and liver tissue extract, although the liver tissue RNase had a different pH maximum than that of the serum RNase.

Adenocarcinoma↗
PubMed1979

A simplified assay for neutrophil chemotactic activity of lymphocyte culture supernatants.

A simplified assay was developed to determine the chemotactic activity of lymphokines for neutrophils in guinea pigs. The neutrophils migrated by chemotaxis to lymphokines through a nucleopore membrane into the lower compartment of the Boyden's chamber and were situated on the bottom cover glass. The number of the cells was counted directly under an inverted microscope. This method is simple, because the staining of the membrane can be omitted. It is also possible to follow the number of responding cells with the time lapsed in each chamber. Furthermore, the method was proved to be sensitive, quantitative and reproducible. The results obtained by this method were compared with those by the conventional methods.

Animals↗