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Biomedical subjects

M Hatano

Publications and source records attributed to M Hatano.

At least 307 records · Page 17Linked to original sources
PubMed1984

[A comparative study of C19 steroid-induced hypertension and deoxycorticosterone acetate (DOCA)-salt hypertension].

Recently, it has been recognized that 19-hydroxyandrostenedione (19-OH-AD), one of the C19 steroids, acts to amplify the mineralocorticoid activity and to elevate blood pressure. However, there is no detailed report about the pressor mechanism and the effects on several humoral depressor factors, i.e., kallikrein, kinin and prostaglandins etc. Therefore, we studied the differences in the pressor mechanisms and the changes in urinary prostaglandin E (PGE) and kinin excretions among deoxycorticosterone acetate (DOCA)-salt hypertension, 19-OH-AD induced hypertension, and testosterone induced hypertension. In this study, DOCA, 19-OH-AD and testosterone were administered subcutaneously to castrated male rats (Wistar rats, 12 weeks) at a dose of 10 mg/body/week for the first 6 weeks. Then the doses were increased to 30 mg/body/week for the next 5 weeks. These experiments were done in metabolic cages. It was found that 19-OH-AD induced hypertension earlier than DOCA and testosterone. However, 19-OH-AD and testosterone had no effect on the levels of urinary kinin and PGE excretions, while DOCA significantly increased urinary kinin and PGE excretions immediately after the onset of hypertension. Furthermore, DOCA increased the urinary Na/K ratio, while 19-OH-AD and testosterone did not change the ratio. It is suggested that 19-OH-AD might induce the pressor action due to the changes in the vascular reactivity rather than the mineralocorticoid activity.

Androstenedione↗
PubMed1984

[The effects of hog pancreatic kallikrein on the plasma kinins-prostaglandins and renin-angiotensin-aldosterone systems].

Recently several reports have been presented stating that glandular kallikrein is present in human and animal blood, and that the oral administration of hog pancreatic kallikrein (HPK) normalises decreased urinary kallikrein and prostaglandin E2 (PGE2) excretions and elevated blood pressure in hypertensive patients. In this study, HPK (2,000 KU/kg body weight) was intramuscularly injected into male rabbits, and several hormones (plasma kinins, plasma PGE, plasma 6-keto PGF1 alpha, plasma thromboxane B2 (TXB2), plasma renin activity (PRA), plasma aldosterone, plasma ACTH) were measured before and after HPK administration in order to clarify the role of glandular kallikrein in the blood. Plasma kinins concentrations were significantly increased (the mean baseline level: 1 +/- 1 pg/ml (mean +/- S.E.), 30 min: 230 +/- 22 (p less than 0.001), 60 min: 288 +/- 36 (p less than 0.001), and 120 min: 130 +/- 9 (p less than 0.001] after HPK administration. Plasma levels of PGE were slightly increased after HPK administration, but the change was not significant as compared with the mean baseline level. Plasma levels of 6-keto PGF1 alpha were significantly increased from the mean baseline level of 229 +/- 38 pg/ml to 594 +/- 131 (p less than 0.05) at 30 min and to 378 +/- 67 (p less than 0.01) at 60 min but were decreased to 278 +/- 37 at 120 min after HPK administration. On the other hand, the changes in plasma TXB2, aldosterone and ACTH concentrations, and PRA were not significant before and after HPK administration. From the present study, it was clarified that the exogenous intramuscular administration of HPK increased plasma levels of kinins and PGI2, but induced no elevation in plasma levels of other hormones including PRA. Therefore, it was concluded in this acute experiment that there was a close relationship between the kallikrein-kinin system and PGs.

6-Ketoprostaglandin F1 alpha↗
PubMed1984

Dexamethasone enhances human cytomegalovirus replication in human epithelial cell cultures.

An epithelial human hepatoma cell line (PLC/PRF/5) and a primary epithelial human baby kidney (HBK) cell culture showed restricted growth of human cytomegalovirus (HCMV). Treatment of these two epithelial cell cultures with dexamethasone greatly enhanced their ability to support HCMV replication. Growth kinetic experiments and infectious center assay revealed that in both the hormone-treated cultures infectious progeny virus appeared earlier by 1 or 2 days and 5- or 10-fold more cells are able to produce infectious virus. There was an approximate 50- or 100-fold increase in virus yield compared to that in the untreated control cultures. Enhanced HCMV replication in the hormone-treated cultures was not due to differences in the cell growth or the virus adsorption and was supported by evidence of increased synthesis of HCMV-specific immediate early antigens and DNA.

Carcinoma, Hepatocellular↗
PubMed1984

[Changes in responses of blood pressure and several hormones to infusions of angiotensin II and angiotensin III in patients with essential hypertension].

In this study, the effects of angiotensin II (A II, Asn-Arg-Val-Tyr-Val-His-Pro-Phe) and angiotensin III (A III, Arg-Val-Tyr-Ile-His-Pro-Phe) on blood pressure (B.P.), pulse rate, several hormones [plasma renin activity (PRA), plasma aldosterone (PA), ACTH, plasma cortisol (PC), urinary catecholamines and urinary aldosterone] and urinary electrolytes were investigated in 9 male patients with essential hypertension [mean age 36.2 +/- 4.1 (S.E.) years]. A II and A III infusions (8 ng/kg/min, 60 min) were started from 0900 h and blood samples were drawn before, at 15, 30, 45, 60 min after the beginning of the infusions, and at 15 min after their cessation. Urinary samples were collected within 2 hrs before and after the infusions, respectively. A II significantly increased B.P.(p less than 0.01) during the infusions, whereas A III did not increase B.P.. PRA significantly decreased after the infusions of A II and A III (p less than 0.05), but the potency of A II was significantly greater than that of A III (p less than 0.01). PA was increased after both infusions, but in response to A III, a peak was observed at 30 min after the infusion and subsequently, the levels decreased gradually. Significant differences between both responses were found at 45 and 60 min (p less than 0.05) after the infusions. ACTH was unchanged during the infusions, but PC was equipotentially suppressed during the infusions, with the suppression of A II being similar to that of A III. In the responses of urinary catecholamines, noradrenaline and dopamine were equipotentially decreased after the infusions (p less than 0.05). The results of the present study clearly indicate that several differences exist between the biological activities of A II and those of A III. Further systematic experimental studies are needed to resolve the details.

Adrenocorticotropic Hormone↗
PubMed1984

Interaction of trifluoperazine with Tetrahymena calmodulin. A 19F NMR study.

We have used 19F NMR to study interactions of trifluoperazine (TFP), a potent calmodulin (CaM) antagonist, with Tetrahymena calmodulin (Tet. CaM). Changes in chemical shift and bandwidth of TFP caused by adding Tet. CaM in the presence of excess Ca2+ were much smaller than those by adding porcine CaM. The spectral features of the TFP-Tet. CaM solution in the presence of excess Ca2+ were quite similar to those of the TFP-porcine CaM solution in the absence of Ca2+. The exchange rate of TFP from Tet. CaM was estimated to be nearly 20 s-1. The TFP-Tet. CaM solution in the absence of Ca2+ showed a pronounced pH dependence of the 19F NMR chemical shift, whereas the solution in the presence of excess Ca2+ showed a smaller pH dependence. Thus, it was suggested that TFP is located near a hydrophilic region of the Tet. CaM molecule in the absence of Ca2+, while TFP is located near a hydrophobic region of the Tet. CaM in the presence of excess Ca2+.

Animals↗
PubMed1984

Relation of HVJ (Sendai virus) production to cell growth phase in persistently infected mouse 3T3 cells.

Using 3T3 mouse fibroblasts persistently infected with temperature-sensitive HVJ (3T3-HVJ pi), the relationship between production of HVJ and cell growth was investigated. In these cells the highest virus release into the culture media occurred from 24 to 48 hours after seeding. The release gradually decreased as the cultures approached confluency, at which time it was reduced below 1-2 per cent of that on day 2 in parallel with the decrease of both cellular DNA and RNA synthesis rates. Comparative examination in growing and resting HVJ carrier 3T3 cells showed that amounts of nucleocapsids and rate of viral structural protein synthesis in the latter phase was reduced to 4-5 per cent of those in the former phase. In addition, viral replication and transcription rates in the resting phase were found to be suppressed to 8-9 per cent of levels detected in the growing phase. These results suggest that the reduced virus production in the resting HVJ carrier cells may be mainly due to the suppression of viral RNA synthesis.

Animals↗
PubMed1984

Antigenic variation of HVJ (Sendai virus) HN glycoprotein detectable by monoclonal antibodies during persistent infection.

Three newly established monoclonal hybridoma antibodies to the haemagglutinin molecule of HVJ, designated A7, B3 and F11, recognize operationally non-overlapping antigenic determinants and have neutralizing activity. Using these antibodies, the frequencies of occurrence of neutralization-resistant antigenic variants were analysed in virus populations released from four cell lines persistently infected with HVJ, namely GM2-HVJ, LLCMK2-HVJ, Vero-HVJ and GEsl-HVJ at various passage stages. Antigenic variants were selected from culture fluids of these HVJ carrier cells at a total frequency of 10(-3.3), 10(-3.8) and 10(-3.6) by monoclonal antibodies A7, B3 and F11, respectively. These values were considerably higher than those of 10(-4.7) to 10(-5.2) detected in a stock preparation of wild-type virus with these antibodies. All the variant viruses isolated as above were negative in neutralization, haemagglutination inhibition and immunofluorescent staining tests with each monoclonal antibody used for their isolation, but were positive with the other antibodies.

Animals↗
PubMed1984

Enhanced replication of human cytomegalovirus in human fibroblasts treated with dexamethasone.

The effect of glucocorticoid hormones on the replication of human cytomegalovirus (HCMV) was studied in human embryonic lung (HEL) cells. Treatment of cells with pharmacological concentrations of adrenal glucocorticoids such as dexamethasone enhanced HCMV replication; treatment with oestrogenic or androgenic hormones did not do so. In dexamethasone-treated HEL cells there was an approximately tenfold increase in virus yield, with the virus eclipse period shortened by 1 day compared to control cultures. Treatment of cells with the hormone also enhanced plaquing efficiency of the virus by approximately tenfold. As the synthesis of virus-specific immediate early proteins and antigens was notably enhanced together with an increase of HCMV DNA synthesis, it appeared that the early stages of the HCMV replication cycle might be under hormonal control. Moreover, the data presented suggest that the hormonal enhancement of HCMV replication involves specific receptor proteins and requires the synthesis of a specific cellular mRNA(s).

Adsorption↗
PubMed1984

Synthesis of M protein of HVJ (Sendai virus) in rat glial cells is selectively restricted at a non-permissive temperature.

Production of HVJ (Sendai virus) wild-type in rat glial cells was characteristically restricted at high temperature. The synthesis of the M protein was selectively decreased in infected cells at 39 degrees C. This temperature-sensitive (ts) character of HVJ was not observed in infection of LLCMK2 cells or chick embryo fibroblast cells. Newcastle disease virus, mumps virus and vesicular stomatitis virus did not show ts growth in glial cells.

Animals↗
PubMed1984

Generalized neonatal herpes virus infection (cytomegalovirus or herpes virus type 1). Comparative examination of loci attacked by two viruses.

Two autopsy cases, a 5-minute-old male infant with congenital cytomegalovirus (CMV) infection and a 10-day-old female infant with herpes simplex virus type 1 (HSV-1) infection, were presented. CMV antibody titer detectable by immunofluorescence (IF) technique was significantly high in the sera of both infant and mother in the CMV case. In another HSV-1 case, we have succeeded in HSV-1 isolation from autopsy liver and subsequent serological identification of the isolated virus, including a detection of high HSV-1 antibody titer in the mother's serum by IF. On light and electron microscopes, these two cases respectively showed typical findings as the severest form of each viral infection. As one of the marked differences between the two cases, the thymus in the HSV-1 case showed remarkable involution with a complete disappearance of thymic cortical lymphocytes. However, the CMV case exhibited an existence of intact thymus without viral injuries. Similar distinguishable differences between the two cases were observed also in the bone marrow. It additionally seems to be unique that cytomegalic inclusion bodies were disclosed fairly well in the submaxillary glands and rarely in the renal tubules even in the case with HSV-1.

Autopsy↗
PubMed1984

Impaired delayed hypersensitivity in lipoid nephrosis.

Cell-mediated immunity (CMI) was evaluated in 76 patients with renal disease by summation of delayed cutaneous hypersensitivity (DHS) responses to 4 test antigens, purified protein derivative (PPD), candida, mumps and keyhole limpet hemocyanin (KLH). Patients with lipoid nephrosis (LN) in the nephrotic stage had less mean induration of skin reactivity and a smaller proportion reacting to the former 3 antigens as compared with normal controls or LN patients without the nephrotic syndrome (NS). In contrast, the intensity of skin reactivity and the frequency of negative reactions in LN patients in remission and chronic mesangial proliferative glomerulonephritis (CGN) were similar to those of the control subjects. Immune response to KLH was also impaired in LN patients with the NS as measured by skin testing. The data indicate an impaired DHS in LN and suggest that the impairment relates to the clinical stage of disease.

Adolescent↗
PubMed1984

Defective cell-mediated immunity in lipoid nephrosis.

Cell-mediated immunity (CMI) was studied in 28 patients with biopsy-proven lipoid nephrosis (LN). The LN patients with nephrotic syndrome (NS) had a significant depression in CMI, characterized by impaired delayed hypersensitivity skin reactivity to purified protein derivative (PPD), depressed local graft-versus-host reaction (GVHR), decreased proportion of T lymphocytes and diminished lymphocyte transformation to phytohemagglutinin (PHA). Concanavalin A (Con A)-induced suppressor cell activity (SCA) was found to be significantly increased in LN patients with NS compared to that in normal individuals. In contrast, the mean levels of CMI and SCA studied in LN patients in remission and in patients with chronic mesangial proliferative glomerulonephritis (CGN) did not differ from normal subjects. Our findings support the notion that at least in some LN patients with the NS, activated suppressor cells are present and possibly account for their decreased CMI.

Adolescent↗
PubMed1984

Defective concanavalin A-induced suppressor cell activity in lupus nephritis.

To determine whether patients with systemic lupus erythematosus (SLE) and active nephritis have more profound defects in suppressor cell activity, we studied concanavalin A (Con A)-induced suppressor cell activity (SCA) in 12 patients with lupus nephritis (LN) and 11 patients with chronic mesangial proliferative glomerulonephritis (CGN) without renal insufficiency. The levels of Con A-induced SCA were decreased in patients with LN compared with those in normal controls and those in CGN patients and lower in LN patients with the nephrotic syndrome (NS) than in those without NS. In contrast, the mean responses of Con-A-induced SCA in CGN patients with or without NS did not differ from normal subjects. These findings may lend further insight into the understanding of the immunoregulatory defect in LN.

Adolescent↗
PubMed1984

Effects of chemical inducers on the Epstein-Barr virus cycle in epithelial/nasopharyngeal carcinoma hybrid cell line.

We have studied the induction of Epstein-Barr virus (EBV)-specific antigens in nasopharyngeal carcinoma hybrid cells by treatment with the tumor promotor 12-0-tetradecanoylphorbol-13-acetate (TPA), hydrogen peroxide (H2O2) and n-butyric acid. The intensity of EBV induction by these drugs was in the order of n-butyric acid----TPA----H2O2. Furthermore, the combination of H2O2 or TPA and n-butyric acid acted synergistically on EBV-induced antigens.

Butyrates↗