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Biomedical subjects

M Hatano

Publications and source records attributed to M Hatano.

At least 289 records · Page 16Linked to original sources
PubMed1985

Abortive infection with human cytomegalovirus induces an alteration of growth pattern: morphological changes with cytocidal effect in rabbit kidney epithelial cells. Brief report.

Rabbit kidney epithelial cells (RK13) exhibited a cytopathic effect (CPE) characterized by cell rounding after infection with human cytomegalovirus (HCMV). Although HCMV-specific immediate early and early antigens were detected by indirect immunofluorescence techniques, neither late antigens nor infectious progeny virus could be observed in virus-infected RK13 cells. HCMV-infected RK13 cells showed a prolonged doubling time and a decreased saturation density in cell growth compared to uninfected control cells. Moreover, colony forming ability (CFA) of virus-infected cells decreased by approximately 70 per cent compared to that of uninfected cells during the first 24 hours after infection. These results indicate that an abortive infection of RK13 cells with HCMV induces an alteration of growth pattern including morphological changes with cytocidal effect.

Animals↗
PubMed1985

Deterioration of chum salmon muscle during spawning migration--VI. Changes in serum protease inhibitory activity during spawning migration of chum salmon (oncorhynchus keta).

A relation between muscle protease activity and serum protease inhibitory activity of chum salmon during spawning migration was studied with regard to their physiological states. The autolytic activity of chum salmon muscle significantly increased, while the trypsin inhibitory activity in serum significantly decreased during spawning migration. Serum trypsin inhibitor was inactivated following treatment with androgen. It was consequently proved that androgen was trigger to the inactivation of serum protease inhibitor, resulting in high levels of muscle protease activity during spawning migration.

Animals↗
PubMed1985

Effects of purified hog pancreatic kallikrein on the kinin-prostaglandin system and renin-angiotensin-aldosterone system.

In the present study, hog pancreatic kallikrein (HPK, 2,000 KU/kg body weight) was intramuscularly injected into male rabbits and several plasma hormones [kinin, prostaglandin E (PGE), 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha), thromboxane B2 (TXB2), plasma renin activity (PRA), aldosterone, and ACTH] were measured before and after the HPK administration, in order to clarify the role of glandular kallikrein in the blood. The plasma kinin concentrations were significantly (p less than 0.001) increased from 1 +/- 1 (mean +/- SE), the baseline level, to 230 +/- 22, 288 +/- 36 and 130 +/- 9 pg/ml at 30, 60, and 120 min, respectively, after HPK administration. The plasma level of PGE were slightly increased after HPK administration, but the change was not significant compared to the mean baseline level. The plasma levels of 6-keto-PGF1 alpha were significantly increased from 229 +/- 38, the baseline level, to 594 +/- 131 (p less than 0.05) at 30 min after the administration of HPK and tended to increase (378 +/- 67) at 60 min after the HPK administration, but fell to 278 +/- 37 pg/ml at 120 min after the administration of HPK. On the other hand, the changes in plasma TXB2, aldosterone, ACTH, and PRA before and after HPK administration were not significant. The present results showed that exogenous intramuscular administration of HPK increased the plasma levels of kinin and prostacyclin, but caused no elevation in the plasma levels of other hormones including PRA. It is concluded, therefore, that in this acute experiment, there was a close relationship between the kallikrein-kinin system and prostaglandins.

6-Ketoprostaglandin F1 alpha↗
PubMed1985

Effects of human interferon on cell proliferation and antigens induced by Epstein-Barr virus in epithelial hybrid cells derived from nasopharyngeal carcinoma.

The effects of human leukocyte interferon (HulFN-alpha) and fibroblast interferon (HulFN-beta) on cell growth and induction of antigens related to Epstein-Barr virus (EBV) in a nasopharyngeal carcinoma hybrid cell line (NPC-KT) were studied. HulFN-alpha showed little antiproliferative and anti-EBV effect on NPC-KT cells. On the other hand, HulFN-beta showed modest antiproliferative effect on NPC-KT cells; the effect was dependent on time and HulFN-beta concentration. HulFN-beta alone was ineffective in inhibiting EBV-related antigens, but NPC-KT cells treated for more than 1 week with HulFN-beta inhibited the action of the chemical inducer of EBV, n-butyric acid, and the appearance of EBV-induced early antigen and EB viral capsid antigen was inhibited more than 50% compared with NPC-KT cells treated with n-butyric acid alone.

Antigens, Viral↗
PubMed1985

Serum and urinary ferritin levels in patients with rheumatoid arthritis.

The serum and urinary ferritin levels in 52 RA patients were measured by the 2-site immunoradiometric assay method. Serum ferritin levels in RA patients correlated with C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) but not with serum iron levels and hemoglobin concentrations, although they were within the normal range. High serum ferritin levels were associated with sera with hyper gamma-globulin and rheumatoid factors. In sequential studies, serum ferritin changed in parallel with ESR, CRP and disease activity in a majority of the patients. The urinary ferritin levels and u/s ratios in some RA patients were higher than control values. Higher values were found particularly in the group of patients under gold therapy but not in groups under other treatments.

Adolescent↗
PubMed1985

Isolation of transforming and early antigen-inducing Epstein-Barr virus from nasopharyngeal carcinoma hybrid cells (NPC-KT).

The biologic activities of Epstein-Barr (EB) virus from an epithelioid-nasopharyngeal carcinoma hybrid cell line (NPC-KT) and three subclones from the NPC-KT cells were examined. Much infectious virus was released by treatment with 5-iodo-2'-deoxyuridine. All virus preparations from NPC-KT cells and the subclones were found to possess both transforming and EB virus-induced early antigen (EA)-inducing activities. The lymphoblastoid cell lines, which were established by infection of human cord-blood lymphocytes with the virus, were diploid with normal karyotypes. The cell lines were positive for EB virus-associated nuclear antigen but negative for EA and EB viral capsid antigen.

Antigens, Viral↗
PubMed1985

Depression of local graft-versus-host reaction in patients with lipoid nephrosis.

The local graft-versus-host reaction (GVHR) following intradermal injection of peripheral blood lymphocytes (PBL) in Lewis rats was examined in order to test the cell-mediated immunity (CMI) in 31 patients with renal disease and in healthy controls. It was found that patients with lipoid nephrosis (LN) in the nephrotic stage and systemic lupus erythematosus (SLE) had less mean lesion of the local GVHR and a smaller proportion with positive reactions as compared to normal subjects. This abnormality in LN patients was correlated well with the degree of delayed hypersensitivity skin test responses to purified protein derivative (PPD) and improved with the onset of clinical remission. In contrast, the mean lesion in PBL from LN patients without nephrotic syndrome (NS) and from chronic mesangial proliferative glomerulonephritis (CGN) did not differ from the normal subjects. These findings raise the question of a selective defect in CMI in LN patients with NS. Our studies also indicate that the local GVHR represents a rapid, clinically useful test for assessing cellular immunocompetence in renal disease.

Adolescent↗
PubMed1985

Impaired T-lymphocyte colony formation in lipoid nephrosis.

The T-colony-forming capacity was examined in 13 normal subjects and 14 patients with biopsy-proven lipoid nephrosis (LN). Eighteen additional patients with other well-defined forms of glomerulonephritis were studied as a disease control. Significantly fewer T lymphocyte colonies were found in LN patients and the nephrotic syndrome (NS) than in normal controls. A similar change could be observed in the groups with other types of NS. LN patients with NS had the lowest values of T-colony-forming cells (TCFC), but there was no statistically significant difference between the groups of nephrotic subjects. It was also of interest that peripheral blood lymphocytes (PBL) from 3 patients with lupus nephritis (SLE) produced significantly low levels of TCFC. The lower levels of TCFC in LN could be enhanced when exogenous interleukin 2 (IL 2) was added to the system. We also examined the production of T-colony-stimulating factor (TCSF). The TCSF production by stimulated PBL from LN patients was lower than in normal subjects. To explain our observations, we presumed that the T colony dysfunction seen in LN patients with NS might in part be due to the decreased TCSF activity.

Adolescent↗