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Biomedical subjects

M Hashimoto

Publications and source records attributed to M Hashimoto.

At least 1,153 records · Page 64Linked to original sources
PubMed1981

Determination of a new hypoglycemic drug, gliclazide, in human serum by radioimmunoassay.

radioimmunoassays have been developed which enable accurate and sensitive determination of gliclazide in human serum. Antisera A and B against gliclazide were obtained from guinea pigs immunized with conjugates A and B prepared by coupling gliclazide homologues, 1-(p-toluenesulfonyl)-3-(4'-carboxypiperidino)urea and 1-(4-methyl-3-carboxybenzenesulfonyl)-3-(3-azabicyclo[3,3,0]oct-3-yl)urea, to bovine serum albumin. 3H-Gliclazide was used as a tracer. Dextran-coated charcoal was used to separate bound and free 3H-gliclazide in the reaction mixture. The assays of gliclazide in serum were possible over a concentration range from 0.25 to 20 microgram/ml with the antiserum A and from 0.1 to 10 microgram/ml with the antiserum B, respectively, using 0.01 ml of human serum without the need for an extraction procedure. The antisera used for the assays were specific for gliclazide. Data obtained by the radioimmunoassay with the antiserum A are in good agreement with those by the radioimmunoassay with the antiserum B and gas-liquid chromatography. Serum levels of gliclazide in healthy volunteers receiving single oral dosing (40 mg/subject) have also been determined.

Antibody Specificity↗
PubMed1981

[Studies on the prostaglandin E2 receptor in human corpora lutea (author's transl)].

It is known that Prostaglandins (PGs) have a specific effect on the corpus luteum function. In this paper we have studied the physical and chemical characteristics of PGE2 receptor in human corpora lutea to explain the mechanism on corpus luteum function. The presence of PGs binding sites in corpora lutea has been reported, but hardly any papers are available on the presence of PGE2 binding sites in human corpora lutea. The present study was undertaken to determine, using the proportion graph method, whether PGE2 receptors are present in human corpora lutea or not. The number of binding sites and association constant of PGE2 receptor to human corpora lutea of the luteal phase of the cycle and gestation were examined. There is evidence (1) that PGE2 receptors were present in human corpora lutea, and the binding sites have one specific and one non-specific binding system. (2) That the number of binding sites in early, mid and late luteal phase corpora lutea were respectively 105-157, 190-196, 162-190 f moles/mg protein. Association constants were 2.0-12.0 x 10(11) M-1. (3) That the number of binding sites of 6th week gestation corpora lutea were 98-100 f moles/mg protein, and those of 12th week gestation were 40-85 f moles/mg protein. Association constants of the gestation corpora lutea were 17.0-20.0 x 10(11) M-1.

Adult↗
PubMed1981

[Body sway stabilization induced during periodic saccades (author's transl)].

Periodic saccadic eye movement has been known to cause a reduction of the body sway while standing upright in man. Then, it was studied that under what experimental conditions such a well-balanced posture could be obtained; with special references to antigravity muscle activity of the lower leg, saccades in the dark, and saccade size and frequency. Body sway was measured as the antero-posterior deviation of the center of gravity. EOG (electro-oculogram) and EMG of soleus, gastrocnemius and tibialis anterior muscles were simultaneously recorded. The results were as follws: 1) The sway reduction was observed during periodic saccades performed in frequency higher than 0.2 Hz at 30 degrees, in amplitude greater than 5 degrees at 0.5 Hz and in duration longer than a few seconds without direction specificity. 2) The effect of saccades on the body sway was classified into three types, each of which was specific to the subject. In type I and II the reduction of the body sway occurred for the component of 0.2-0.4 Hz. The sway reduction was accompanied with the increase in activity of soleus and gastrocnemius. The sway reduction was also accompanied with a forward shift of the body position in type I, but not in type II. Type III showed active discharges in soleus and gastrocnemius before saccades, suggesting that the body was being already inclined during fixation of eyes. In this type no sway reduction and no continuous shift of the body position were observed. 3) The sway reduction was observed also during periodic, voluntary rapid eye movement in complete darkness. 4) Continuous eye rotation in either direction did not affect the body sway. These results suggest that some neural activty related to the saccade may flow in the extrapyramidal system, raised the activity of triceps surae muscle directly or indirectly and stabilize the standing posture.

Adolescent↗