Search PubMed⌕ Search

Biomedical subjects

M Harada

Publications and source records attributed to M Harada.

At least 1,297 records · Page 72Linked to original sources
PubMed1978

Dipeptidyl aminopeptidase IV, a glycoprotein from pig kidney.

Dipeptidyl aminopeptidase IV was purified 350 fold from pig kidney by chromatographic procedures including affinity chromatography with conjugates of Gly-Pro linked to Sepharose 4.B. Purified enzyme existed in a dimeric form as determined by sodium dodecyl sulfate polyacrylamide-gel electrophoresis using dimethyl suberimidate (a cross-linking reagent). The molecular weight of the subunit was estimated to be 100 000 by gel filtration with 6 M guanidine hydrochloride and to be 94 000 based on analysis of N-terminal residue (dinitrophenyl-serine). The amino acid composition of the purified enzyme was also determined. The enzyme contained 18.3% of carbohydrate consisting of mannose, galactose, fucose, glucosamine and sialic acid. The enzyme desialized with sialidase was found to still possess full enzyme activity.

Amino Acids↗
PubMed1978

Viability of T lymphocytes survived in frozen-thawed human bone marrow.

To know immunocompetence of cryopreserved human bone marrow, an optimal condition for cryopreservation of hemopoietic stem cells was determined on the basis of in vitro colony formation. Viability or immune function of frozen-thawed bone marrow lymphocytes was evaluated by spontaneous rosette formation and sheep red blood cells and mixed lymphocyte culture activity. It was demonstrated that the thawed marrow cells could form E rosettes and respond to alloantigens in mixed lymphocyte culture equally or more than those before freezing. Stimulating activity of cryopreserved marrow cells in mixed lymphocyte culture was not altered through a freeze-thawing process. These observations suggest that bone marrow lymphocytes may survive without losing their immunocompetence after freezing as well as hemopoietic stem cells.

Bone Marrow Cells↗
PubMed1978

Role of gastric motility in development of stress-induced gastric lesions of rats.

Gastric motility of stressed rats was studied to determine its role in producing stress-induced gastric lesions. Restraint and water immersion resulted in an increase in gastric motility which consisted of an increase in frequency and amplitude of contractions and a rise in gastric tone. This increase reached maximal levels 2 to 4 hr after stress, and persisted thereafter. Formation of gastric lesions was markedly accelerated after occurrence of the increased gastric motility. In contrast, restraint alone neither produced such a vigorous increase in gastric motility, nor were the gastric lesions severe. A continuous infusion of papaverine during restraint and water immersion inhibited increase in frequency and amplitude of gastric contractions and prevented formation of gastric lesions. It is concluded that increased gastric motility is closely associated with marked formation of gastric lesions under conditions of restraint and water immersion stress and is probably a main cause for their vigorous formation, although formation of lesions occurs to a small degree without involvement of gastric motility.

Animals↗
PubMed1978

Effect of dietary methionine level on anserine and carnosine contents in the gastrocnemius muscle of rat.

Anserine and carnosine in the muscle of growing rats were measured to explore the effect of dietary methionine. Carnosine was reduced in the gastrocnemius muscle of rats fed on a methionine-free diet. After refeeding on methionine for one week, carnosine in the muscle approached to the normal level. In contrast, anserine increased in the muscle of rats fed on a methionine-free diet. Anserine also increased in the gastrocnemius muscle of rats fed on a methionine-excess diet, whereas carnosine did not vary. Npi-Methylhistidine was detected in the muscle of rats fed on a methionine-excess diet. Moreover, Npi-methylhistindine and Ntau-methylhistine in the urine increased in the rats fed on both methionine-excess and methionine-free diets. Carnosine in the gastrocnemius muscle was not affected by a ethionine diet nor by a orotic acid diet which induced fatty liver. However, anserine increased in the muscle of rats fed on a ethionine diet.

Amino Acids↗