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Biomedical subjects

M Harada

Publications and source records attributed to M Harada.

At least 1,315 records · Page 73Linked to original sources
PubMed1977

Anserine and carnosine contents in muscular tissue of rat and rabbit.

The effect of various muscle conditions on anserine and carnosine contents suggests that these peptides have some physiological role in muscular functions. These conditions are: 1) Although the anserine content in rat gastrocnemius muscle was not changed by ischiamic denervation, the carnosine content was significantly decreased. Carnosinase activity in the denerved gastrocnemius muscle was two times stronger than that of intact gastrocnemius muscle. 2) Carnosine content in rat gastrocnemius muscle was also decreased by forced swimming exercise. 3) Neither anserine nor carnosine was detected in sarcoma and granuloma. 4) In rabbit, anserine and carnosine contents in white muscle fibers were 12-17 and 1-2 mumole/g of wet tissue and were approximately 10 and 2 times more than those in red muscle fibers, respectively.

Animals↗
PubMed1977

T cell function detected in murine bone marrow cells.

Mouse bone marrow cells were fractionated by BSA discontinuous density gradient centrifugation, and a small lymphocyte rich fraction was obtained at the high density. Cells of this fraction were shown to respond in vitro to T cell mitogens and alloantigens. Furthermore, they were able to mount a graft-versus-host reaction when assessed by spleen weight assay and by the method of inhibiting erythroid cell growth by allogeneic lymphoid cells. The results indicate that these lymphocytes possess T cell functions. On the other hand they were found to carry only little theta antigen assessed by the cytotoxic test and by the absorption test. It is presumed, therefore, that the amount of theta antigen on a cell might not correlate with T cell functions, and these lymphocytes might be mature ones in the course of postthymic maturation. Hemopoietic stem cells were determined by spleen colony formation and the peak of colony-forming efficiency was seen at the low density. These observations imply that immunocompetent cells causing GVHR can be separated from hemopoietic stem cells. This procedure may be applied for prevention and reduction of GVHR in allogeneic bone marrow transplantation in human.

Animals↗
PubMed1977

Active rosette forming cells as a possible functional subpopulation of human peripheral T lymphocytes.

Spontaneous E rosette forming cells (RFC) and active E rosette forming cells (ARFC) were separated individually by the rosette sedimentation technique. The responses of these cells to phytohemagglutinin (PHA) and concanavalin A (Con A) were studied. RFC exceeded ARFC in their responses to PHA at all concentrations of PHA and the maximum PHA response of RFC was significantly higher than that of ARFC. On the other hand, Con A appeared to stimulate RFC and ARFC equally at the level of an optimal dose. Therefore, the binding affinity for sheep red blood cells (SRBC) by T lymphocytes and their responsiveness to PHA stimulation may be inversely related. It is also suggested that the PHA-sensitive population may be different from the Con A-sensitive one, or that there may be heterogenous populations among human T lymphocytes in their mitogenic responses. Thus, it is possible that ARFC may be a functional subpopulation of human T cells. An additional finding is that spontaneous DNA synthesis of rosetting cells was significantly lower than that of non-rosetting cells.

Concanavalin A↗