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Biomedical subjects

M Gheorghiu

Publications and source records attributed to M Gheorghiu.

At least 109 records · Page 6Linked to original sources
PubMedDate not supplied

Immuno BCG Pasteur F vaccine.

Immuno BCG Pasteur-F (fresh) vaccine is prepared from young cultures, 7-8 days old. Suspended in Dubos' modified medium it may be preserved for 3 months at 4 degrees C or for many years at--70 degrees C. Tests with the vaccine for colony-forming units during storage, biological controls in animals and allergy in children have demonstrated its good stability.

Animals↗
PubMedDate not supplied

[Clinical trial of amoxycillin in chronic bronchitis and bronchial dilatations].

The therapeutic results were followed of amoxycilline after application in 57 cases of chronic bronchitis and bronchial dilatations that had been treated previously with antibiotics (including ampicillin--in 37 cases). The amount of amoxycilline was of 2 g per day, given in four 0,5 g doses. A total of 23,3 percent "very good" results were obtained (expectoration either disappeared completely or was reduced to 2--3 ml per day), and 40,4 percent "good" results (with expectoration down to less than one half of the initial amount). Thus in two-thirds of the cases the results were favourable. The cases that did not respond to ampicilline gave only 10,8 percent "very good" results but the "good" results represented 51,3 percent, indicating an increased efficiency of the new drug as compared with ampicilline. The serum levels of Amoxycilline (9,4 mg/ml after 2 hours, and 4,4 mg/ml after 4 hours), when a 0,5 g dose was given every 6 hours, assure a continuous and efficient bacteriostatic concentration. No significant adverse reaction were noted.

Amoxicillin↗
PubMedDate not supplied

Dysglobulinemias revealing rheumatoid arthritis.

Fifteen patients (with dysglobulinemia, ranging in age from 35 to 52 years, were studied over a period of 6 years. The following parameters of immune response and immunoelectrophoresis of serum proteins were checked: total hemolytic complement (CH50), serum cryoglobulins, presence of circulating immune complexes (CIC), beta 2-microglobulins, rheumatoid factor (RF). Of the 15 patients investigated, 6 (40%) developed during the period of study severe forms of rheumatoid arthritis (RA) which required the introduction of continuous cortisone therapy. In a control group, ranging between the same age limits but without immune disturbances, no case of RA was observed. It should be noted that polyclonal hypergammaglobulinemia preceded by at least 5 years the appearance of articular inflammation. The changes of serum complement were also significant. CH50 was found constantly decreased below 40 HU/ml, particularly in the patients with febrile forms of RA; positive latex fixation test greater than 1/80 was observed at least 6 months before the appearance of articular tumefactions. Increase of beta 2-microglobulins in the serum and the presence of cryoglobulins was also observed in the 4 patients with RA. Dysglobulinemia has often been observed in the course of RA but it has seldom been presented as a preceding disturbance predicting the disease. The originality of the observations reported in this paper consists in a case of malignant RA appeared in the course of dysglobulinemia.

Adult↗
PubMedDate not supplied

Particularities of rheumatoid arthritis in aged people.

Forty patients with rheumatoid arthritis (RA) selected according to the ARA criteria, in whom the onset of disease had appeared after the age of 65 years, were studied comparatively with a control group of 40 RA patients in whom the disease had occurred before this age. Every patient was subjected to a complex clinical and biologic investigation. The characteristics of the two groups are presented. The onset of disease in old age was frequently acute (65% of the cases as compared with 45% in the control group). The difference between the two groups was statistically significant from this viewpoint (p less than 0.001). Presence at onset of certain general manifestations associated with loss of weight, risomelic involvement and association of Horton disease were found in 50% of the old agers as compared with 12.5% in the control group. The radiologic aspect was similar in both groups. Positive rheumatoid factor in 64% of the cases, as well as the homogeneous or speckled antinuclear factor were likewise comparable with the data in the younger group. From the evolutive point of view RA with onset in old age showed good response to therapy. Chrysotherapy was effective in 80% of the patients in the old age group and in less than 50% of the controls.

Adult↗
PubMedDate not supplied

[Effect of diffuse light and heat on 8 BCG vaccines prepared from 4 different strains].

In order to improve our knowledge of the effect of daylight on freeze-dried and rehydrated BCG, 8 BCG vaccines prepared with four BCG strains--Danish 1331, French 1173-P2, English (Glaxo-1077) and Japanese 172--were studied. Four of them were in amber-coloured ampoules manufactured in Copenhagen (K. Bunch -Christensen) and four were in colourless ampoules manufactured by Institut Pasteur Production (Paris). Samples of these vaccines, both freeze-dried and rehydrated for use in children, were exposed to both factors and checked regularly for their viability. Daylight had a deleterious effect on the viability of both freeze-dried and (especially) rehydrated BCG vaccines. The fall in viability began after 2-h exposure of rehydrated vaccines and after 8-h exposure of freeze-dried ones. BCG survival was improved by coloured ampoules. Differences were observed between BCG strains in terms of the light effect, with the French strain being most resistant, the Danish least resistant and the others intermediate. UV irradiation was even more deleterious: 50% survival after 30-min exposure at 20 microW /cm2. In conclusion, since BCG vaccines are sensitive to these factors, they must therefore be protected and their viability checked during BCG vaccination campaigns.

BCG Vaccine↗
PubMedDate not supplied

[Stability and immunogenicity of the fresh BCG vaccine applied with a new multiple puncture device (author's transl)].

A new multiple puncture instrument with interchangeable pieces has been devised. It permits the application of 12 or 28 cutaneous punctures, depending on the age of the patient (child). Fresh concentrated (50 mg/ml) BCG vaccine was distributed either into neutral glass ampoules or into neutral polypropylene tubes. No difference in viability was observed after storage at 4 degrees C and in a series of 10 batches of vaccine, a good survival (about 50%) was found after three months. Also, survival was identical among 9 batches of vaccine, whether it was resuspended in a protective solution containing human albumin plus 5% glycerol or in 6% glycerol only. The immunogenicity of the fresh concentrated vaccine, when applied with the new multiple puncture device, was studied using different tests: --the growth of BCG in organs of mice, --the survival time after virulent challenge of vaccinated versus unvaccinated mice, --tuberculin-delayed hypersensitivity of vaccinated guinea-pigs. The immunogenic potency has been found similar to that observed using other methods of BCG vaccination. These experimental results confirm the merit of the new method of vaccination using the multiple puncture device.

Animals↗
PubMedDate not supplied

Reduction of potassium tellurite and ATP content in Mycobacterium leprae.

The purpose of this investigation was to examine those properties of Mycobacterium leprae which might be useful in estimating the heterogeneity of the bacterial populations harvested from the tissues of experimentally infected armadillos. The following technical procedures were applied: fluorescent microscopy on smears stained by an Auramine O-Ethidium bromide dual procedure, fine structure observation of ultrathin sections, reduction of potassium tellurite as observed under the electron microscope, and ATP content of the bacteria. The quantitative data from these procedures was compared to the morphological index, and it was shown that the results did not correlate. However, the study of tellurite reduction was interesting because this technique may prove useful in evaluating the contamination of M. leprae preparations by host tissues. More significant was the fact that M. leprae reduced tellurite. Even though the reduction was at a very low efficiency under the conditions described, it would be possible to use this technique to investigate the effects of several substrates on the respiratory activity of the leprosy bacilli.

Adenosine Triphosphate↗
PubMedDate not supplied

[The pathogenesis of diabetic dyschromatopsia].

Essential data about the physiology of colour vision are reminded, with a stress layed on the possible contribution of the rodes in perception of blue and green colours. The main features of the diabetic dyschromatopsia are reviewed, together with their pathomorphological support and the two existing theories regarding its pathogenesis: vascular origin versus neuronal origin of the defect. The contribution of the photocoagulation to these alterations is also discussed.

Animals↗