Search PubMed⌕ Search

Biomedical subjects

M Fukuda

Publications and source records attributed to M Fukuda.

At least 1,243 records · Page 69Linked to original sources
PubMed1984

Endoscopic sonography of the liver--diagnostic application of the echolaparoscope to localize intrahepatic lesions.

To overcome the limitations of ultrasonography and laparoscopy for abdominal diseases, the use of echolaparoscopy was investigated. The instruments used were the prototype echolaparoscope type 1-3 manufactured by Olympus Optical Co., Tokyo, Japan. The first prototype has the external diameter of 12 mm. It consists of an internal shaft connecting to a fixed transducer and a rotating mirror connected to the motor unit and a regular laparoscope to assess the position of the scanning head. The second and third prototypes were versions of the former and had an outer diameter of 10 mm to allow interchangeable use with a regular laparoscope. Transducers used were either of 10 or 7.5 MHz, 7 mm in diameter, and ultrasonic scanning was made by the mirror reflection method. A total of 67 cases with various abdominal diseases were examined on 73 occasions. The merit of the method is its ability to visualize occult lesions in the liver such as cysts and tumors. Differentiation between hepatic hemangioma and hepatocellular carcinoma could be mad without difficulty due to its increased resolution. Furthermore, this method was used as a guide to liver biopsy of occult tumors and decision making in cases of surgical resection of the hepatic lesions.

Adult↗
PubMed1984

Fine structure of Leucocytozoon caulleryi during microgametogenesis and fertilization.

The fine structure of microgametogenesis and fertilization in Leucocytozoon caulleryi was studied. When the peripheral blood of chickens containing mature gametocytes was withdrawn or ingested by Culicoides arakawae , differentiation of the microgametocytes began immediately. The cytoplasm of host cell and the outer membrane of the microgametocyte disappeared. Axoneme formation began in the cytoplasm and the condensed chromatin masses were observed at the periphery of the nucleus of microgametocyte after disappearance of the nuclear membrane. The axonemes and chromatin masses migrated to the surface of the microgametocyte and participated in the formation of flagellar buds. The flagellar buds passed through interruptions of the inner membrane and were surrounded by the outer membrane of the microgametocyte . The microgametocyte formed eight slender microgametes. The microgamete was a long undulated thread-like structure and composed of a single axoneme and a nucleus. The nucleus was located at the mid to anterior region of the microgamete . The axoneme consisted of nine peripheral doublets arranged around two central microtubules. Penetration of the macrogamete by the microgamete occurred rapidly after ingestion. The axoneme and nucleus of the microgamete were seen free in the cytoplasm and nucleus of a fertilized macrogamete.

Animals↗
PubMed1984

Endoscopic ultrasonography in the diagnosis of pancreatic carcinoma. The use of a liquid-filled stomach method.

To overcome limitation of ultrasound diagnosis of pancreatic diseases, especially of malignancy, the use of an echoendoscope was investigated. The instruments were the prototype echoendoscopes type 1-3 manufactured by Olympus Co., Tokyo, Japan. The first prototype was a mirror-type radial sector scanner equipped to a gastrofiberscope . The 2nd and 3rd types were of similar construction except the use of a small transducer with an increased frequency (7 mm in diameter, 7.5 MHz) and of direct rotation of the transducer connected to the flexible shaft. To visualize the pancreas effectively, a liquid-filled stomach method to use deaerated water in sufficient quantity was employed. As a result, complete visualization of the whole pancreas could be accomplished mainly from the gastric lumen via the gastric wall. Though the number of cases examined were still limited, ultrasonic differential diagnosis between benign and malignant lesions could be made much more clearly than those by currently used ultrasonic scanning.

Endoscopy↗
PubMed1984

[Clinical efficacy of a quadruple combination chemotherapy with ACNU, adriamycin, methotrexate, and prednisolone for patients with non-Hodgkin's lymphoma, who were refractory to VEPA (P) treatment].

A quadruple combination chemotherapy with ACNU, adriamycin, methotrexate and prednisolone (AAAP), was performed on 9 patients with non-Hodgkin's lymphoma, who had been previously treated with a VEPA (P) regimen. Complete remission rate was 44% (4/9) and, in four other patients, the tumor was decreased in size. Complete remission duration was from 30 days to over 390 days. Median survival time was 105 days. Myelosuppressive toxicity was severe. In eight patients, WBC counts were less than 3,000/mm3. In six patients, platelet counts were less than 100,000/mm3. An AAAP regimen is useful in relapse or for resistant forms of non-Hodgkin's lymphoma.

Adult↗
PubMed1984

New method for local drug delivery using resorbable base material in periodontal therapy.

A new method for local drug delivery to the subgingival plaque was developed using hydroxypropylcellulose (HPC) as a base material. Using this material prepared in the form of strips containing antimicrobial drugs, the clinical and microbiological effects by this method on the human periodontal disease were studied. Before the clinical trials, the rate of the drug release from the strips was studied. Both drugs used in this study (CH: chlorhexidine and TC: tetracycline) were released almost within 2 hours from the strips in vitro. In the gingival crevicular fluid, however, it was found that the TC was maintained for 24 hours after the local delivery. Five patients who had deep pockets contralaterally were selected. The CH-containing HPC strips (5%) were applied in one pocket of each patient and the placebo strips were applied in other pocket on day 0, 2 and 4. Plaque Index, Gingival Index, probing depth and the presence or absence of bleeding on probing were recorded on day 0, 2, 4 and 6. Marked reduction of bleeding on probing was found in the pockets applied the CH-containing HPC strips. There was a significant reduction in the proportion of Bacteroides asaccharolyticus in these pockets (p less than 0.01).

Anti-Infective Agents↗
PubMed1984

The presence of G1 and G2 populations in normal epithelium of rat urinary bladder.

The cell population kinetics of transitional epithelium of the rat urinary bladder was analysed by (3H) thymidine autoradiography and pararosanilin Feulgen DNA cytofluorometry. By flash and 72 h continuous DNA labelling, the generative cells of the transitional epithelium were found to be well localized in the basal layer, and it was postulated that che cells produced by cell proliferation in the basal layer would migrate towards the surface, maintaining direct attachment to the basement membrane by anchorage of a cellular process. Analyses of normal and wounded transitional epithelium revealed that 58.8% of all basal cells are G0 cells in G1 phase (G1-population), and 59.0% of the remaining basal cells reside in prolonged (75.1-108.0 h) G2 phase, preserving the ability to divide (G2-population). The cell cycle time of the generative basal cells including the long G2 phase was calculated as 129.1-162 h. All the cells existing in upper layers were found to be also G0 cells in G1 phase, with the DNA amounts of 2C class. No polyploid cells could be detected except for 2C-2C binucleated cells in the superficial layer. The existence of a G2-population may serve for the urgent need of cell incrementation to repair cell loss as the cells in G2 phase can divide without the time-delay needed for DNA synthesis. The rat transitional epithelium, which is composed exclusively of proliferating and potentially proliferative cells, will have much greater capability to repair damage than stratified squamous epithelia.

Animals↗