Search PubMed⌕ Search

Biomedical subjects

M Fukuda

Publications and source records attributed to M Fukuda.

At least 1,099 records · Page 61Linked to original sources
PubMed1987

Decrease in the c-myb gene transcript during differentiation of mouse teratocarcinoma stem cells.

Transcript of the c-myb gene, one of the proto-oncogenes, was clearly detected in undifferentiated mouse F9 teratocarcinoma stem cells, but not in terminally differentiated mouse parietal endoderm PYS-2 cells. As F9 cells can be induced to differentiate into parietal endoderm-like cells by the addition of retinoic acid and dibutyryl cAMP, we examined levels of the c-myb transcript under this experimental condition and found that the c-myb transcript was decreased significantly. Thus, a decrease in the c-myb transcript is probably related to early differentiation of mammalian cells.

Animals↗
PubMed1987

Detection of mutagenic compounds in the urine of mice administered pyrene during exposure to NO2.

The urine of mice injected intraperitoneally with pyrene during exposure to NO2 was found to contain highly mutagenic compounds by means of the Ames test using Salmonella typhimurium strain TA98. The mice were exposed to 20 ppm NO2 for 3 days before intraperitoneal injection of pyrene (800 mg/kg of body weight). The pyrene-treated mice were further exposed to NO2 for an additional 24 hr, and the urine from the mice was collected in ice-cooled containers and stored frozen in the dark. The collected samples were treated with beta-glucuronidase and passed through activated Sep-Pack C18 cartridges. After elution with methanol, the effluent was concentrated and the residue was dissolved in dimethyl sulfoxide (DMSO). The DMSO solution was fractionated by high-performance liquid chromatography and the mutagenicity of each fraction was assayed with S. typhimurium strain TA98. The mutagenic compounds 3-hydroxy-1-nitropyrene, 6-hydroxy-1-nitropyrene, 8-hydroxy-1-nitropyrene, and 1-hydroxypyrene were identified in the mutagenic fractions by mass spectrometry and UV-visible spectrophotometry with synthetic reference substances. These mutagenic compounds may have been formed by either nitration of hydroxylated pyrene, or hydroxylation of 1-nitropyrene, which is formed in vivo from pyrene and NO2, or the simultaneous occurrence of these two reactions in the mouse body.

Animals↗
PubMed1987

[Long-term follow-up study of renal autotransplantation in reno-vascular hypertension].

Therapeutic effects of heterotopic renal transplantation for renovascular hypertension were evaluated in ten patients with a follow-up period of more than three years. These ten patients consisted of 4 males and 6 females with the range of age from 21 to 55. The causes of renovascular hypertension were fibromuscular dysplasia in five patients, atherosclerosis in 3 and aortitis syndrome in 2. All the ten patients had a unilateral stenosis of the renal artery. At 3 years after operation, the blood pressure fell to normal range without any medication in 9 patients (90%) and it could be controlled with antihypertensive drugs in the remaining one patient. Operative mortality or deterioration of graft function was not experienced in any of them. One of the female patients had three deliveries after operation. The heterotopic renal autotransplantation is a therapeutic method to be selected for renovascular hypertension, since excellent and stable long-term results can be achieved.

Adult↗
PubMed1987

Monoclonal antibodies against human hematopoietic survival and growth factor.

Mouse hybridoma clones were isolated, and found to constitutionally secrete monoclonal antibody (MoAb) immunoglobulins specific for human hematopoietic stem cell growth factor (SCGF). MoAbs were initially screened by dot-blotting and enzyme immunoassay (EIA), and their specificity was confirmed using western blotting and EIA. Protein A-purified MoAb IgG neutralized SCGF activity, namely, granulocyte-macrophage colony potentiating and erythroid burst-promoting activities. The availability of MoAb could extend its application to one-step immunoadsorbent affinity-purification of SCGF as well as analysis on its receptor.

Antibodies, Monoclonal↗